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John Hancock's Outputs (130)

Preharvest application of hydrogen sulfide and nitric oxide improves floral traits and postharvest performance of cut gladiolus inflorescences (2023)
Journal Article

Gladiolus is a well-known bulbous plant producing impressive cut spikes. Hydrogen sulfide and nitric oxide are vital signaling molecules for the proper functioning of plant metabolism. Preharvest applications of these molecules to crops have gained a... Read More about Preharvest application of hydrogen sulfide and nitric oxide improves floral traits and postharvest performance of cut gladiolus inflorescences.

The therapeutic potential of oxyhydrogen gas in oncology: A study on Epstein–Barr Virus-immortalised B-lymphoblastoid (TK6) cells (2023)
Journal Article

Cancer is a leading cause of mortality worldwide. B-cells are a keystone of the adaptive immune response and are essential for the presentation of tumor-associated antigens to various types of T-cells. Approximately 1.5% of global cancer cases, inclu... Read More about The therapeutic potential of oxyhydrogen gas in oncology: A study on Epstein–Barr Virus-immortalised B-lymphoblastoid (TK6) cells.

Hydrogen incorporation into solvents can improve the extraction of phenolics, ‎flavonoids, anthocyanins, and antioxidants: A case-study using red beetroot (2023)
Journal Article

Different methods are used for extracting phytochemicals from plant produce. Some ‎methods require sophisticated and expensive instruments, while others need multiple steps ‎and specialist equipment, leading to an increase in energy, solvents, cost,... Read More about Hydrogen incorporation into solvents can improve the extraction of phenolics, ‎flavonoids, anthocyanins, and antioxidants: A case-study using red beetroot.

The early history of hydrogen and other gases in respiration and biological systems: Revisiting Beddoes, Cavallo, and Davy (2023)
Journal Article

In 2007 a paper in Nature Medicine sparked a new wave of interest in the use of molecular hydrogen (H2) for medical treatments. Since then there has been a flurry of papers looking at a range of medical aspects, from neurodegenerative disease to spor... Read More about The early history of hydrogen and other gases in respiration and biological systems: Revisiting Beddoes, Cavallo, and Davy.

Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily (2022)
Journal Article

Hydrogen gas (molecular hydrogen, H2) has significant effects in a range of organisms, from plants to humans. Many inert gases have been reported to have similar effects, and such responses may be most pronounced when cells are stressed. Xenon (Xe),... Read More about Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily.

Phytochrome B enhances seed germination tolerance to high temperature by reducing S-nitrosylation of HFR1 (2022)
Journal Article

Light and ambient high temperature (HT) have opposite effects on seed germination. Light induces seed germination through activating the photoreceptor phytochrome B (phyB), resulting in the stabilization of the transcription factor HFR1, which in tur... Read More about Phytochrome B enhances seed germination tolerance to high temperature by reducing S-nitrosylation of HFR1.

Do SNPs in glutathione S-transferase-omega allow predictions of the susceptibility of vertebrates to SARS-CoV-2? (2022)
Journal Article

Infection with the SARS-Cov-2 virus causes COVID-19 in humans, and is the cause of the pandemic around the world in 2020 and on. However, some animals have been found to be susceptible to the virus too. This has included the non-human primates, dogs,... Read More about Do SNPs in glutathione S-transferase-omega allow predictions of the susceptibility of vertebrates to SARS-CoV-2?.

Nitric oxide-releasing nanomaterials: From basic research to potential biotechnological applications in agriculture (2022)
Journal Article

Nitric oxide (NO) is a multifunctional gaseous signal that modulates the growth, development and stress tolerance of higher plants. NO donors have been used to boost plant endogenous NO levels and to activate NO-related responses, but this strategy i... Read More about Nitric oxide-releasing nanomaterials: From basic research to potential biotechnological applications in agriculture.

Oxy-hydrogen gas: The rationale behind its use as a novel and sustainable treatment for COVID-19 and other respiratory diseases (2021)
Journal Article

Oxy-hydrogen gas (HHO) is a gaseous mixture of molecular hydrogen (H2) and molecular oxygen (O2) that is generated by the electrolysis of water and delivered in a 2:1 ratio (66% and 33%, respectively) through the use of non-invasive inhalation device... Read More about Oxy-hydrogen gas: The rationale behind its use as a novel and sustainable treatment for COVID-19 and other respiratory diseases.

Leaf arginine spraying improves leaf gas exchange under water deficit and root antioxidant responses during the recovery period (2021)
Journal Article

© 2021 Elsevier Masson SAS Arginine (Arg) metabolism is associated with many cellular and developmental processes in plants and proline, nitric oxide (NO) and polyamines (PAs) have a wide range of physiological functions in plants, including increase... Read More about Leaf arginine spraying improves leaf gas exchange under water deficit and root antioxidant responses during the recovery period.

The effects of seed priming with sodium hydrosulphide on drought tolerance of sunflower (Helianthus annuus L.) in germination and early growth (2020)
Journal Article

© 2020 Association of Applied Biologists Sunflower seeds (hybrid Luka) were primed with water (hydropriming) or sodium hydrosulphide (NaHS) solutions (0.1, 0.5, 1.0 and 1.5 mM NaHS) and subsequently dried to initial moisture content. Unprimed (contro... Read More about The effects of seed priming with sodium hydrosulphide on drought tolerance of sunflower (Helianthus annuus L.) in germination and early growth.

An overview of SARS-CoV-2 (COVID-19) infection and the importance of molecular hydrogen as an adjunctive therapy (2020)
Journal Article

SARS-CoV-2 is an emerging β-coronavirus that causes COVID-19 disease that manifests primarily as a pulmonary infection that can rapidly progress into severe and acute respiratory distress in susceptible patients. Initial reports of severe pulmonary i... Read More about An overview of SARS-CoV-2 (COVID-19) infection and the importance of molecular hydrogen as an adjunctive therapy.

Recommendations on terminology and experimental best practice associated with plant nitric oxide research (2019)
Journal Article

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust Nitric oxide (NO) emerged as a key signal molecule in plants. During the last two decades impressive progress has been made in plant NO research. This small, redox-active molecule is no... Read More about Recommendations on terminology and experimental best practice associated with plant nitric oxide research.

Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells (2018)
Journal Article

© 2018 The Author(s). Background: Mounting evidence suggests that one of the ways that cells adapt to hypoxia is through alternative splicing. The aim of this study was firstly to examine the effect of hypoxia on the alternative splicing of cancer as... Read More about Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells.

Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries (2017)
Journal Article

© 2017 Zafra, Carmona, Traverso, Hancock, Goldman, Claros, Hiscock and Alche. The olive tree is a crop of high socio-economical importance in the Mediterranean area. Sexual reproduction in this plant is an essential process, which determines the yiel... Read More about Identification and functional annotation of genes differentially expressed in the reproductive tissues of the olive tree (Olea europaea L.) through the generation of subtractive libraries.

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans (2017)
Journal Article

© 2017, The Author(s). This study investigated the neurological effects of residual ground-water levels of thiaclopridon the non-target organism Caenorhabditis elegans. Nematodes treated with thiacloprid showed a dose-dependent and significantly incr... Read More about Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans.

S-nitrosoglutathione spraying improves stomatal conductance, Rubisco activity and antioxidant defense in both leaves and roots of sugarcane plants under water deficit (2017)
Journal Article

© 2017 Scandinavian Plant Physiology Society Water deficit is a major environmental constraint on crop productivity and performance and nitric oxide (NO) is an important signaling molecule associated with many biochemical and physiological processes... Read More about S-nitrosoglutathione spraying improves stomatal conductance, Rubisco activity and antioxidant defense in both leaves and roots of sugarcane plants under water deficit.

Harnessing evolutionary toxins for signaling: Reactive oxygen species, nitric oxide and hydrogen sulfide in plant cell regulation (2017)
Journal Article

© 2017 Hancock. During the early periods of evolution, as well as in niche environments today, organisms have had to learn to tolerate the presence of many reactive compounds, such as reactive oxygen species, nitric oxide, and hydrogen sulfide. It is... Read More about Harnessing evolutionary toxins for signaling: Reactive oxygen species, nitric oxide and hydrogen sulfide in plant cell regulation.

The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries (2015)
Journal Article

© 2015 Manoharan et al. The bacterial plant pathogen Pseudomonas syringae pv. phaseolicola (Pph) colonises the surface of common bean plants before moving into the interior of plant tissue, via wounds and stomata. In the intercellular spaces the path... Read More about The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries.

The evolutionarily conserved multifunctional glycine-rich RNA-binding proteins play key roles in development and stress adaptation (2015)
Journal Article

© 2014 Scandinavian Plant Physiology Society. The class IV glycine-rich RNA-binding proteins are a distinct subgroup within the heterogenous superfamily of glycine-rich proteins (GRPs). They are distinguished by the presence of an RNA-binding domain... Read More about The evolutionarily conserved multifunctional glycine-rich RNA-binding proteins play key roles in development and stress adaptation.

Electrochemical detection of extracellular hydrogen peroxide in Arabidopsis thaliana: A real-time marker of oxidative stress (2013)
Journal Article

An electrochemical approach to directly measure the dynamic process of H2O2 release from cultures of Arabidopsis thaliana cells is reported. This approach is based on H2O2 oxidation on a Pt electrode in conjunction with continuous measurement of samp... Read More about Electrochemical detection of extracellular hydrogen peroxide in Arabidopsis thaliana: A real-time marker of oxidative stress.

Low dose ionizing radiation produces too few reactive oxygen species to directly affect antioxidant concentrations in cells (2012)
Journal Article

It has been hypothesized that radiation-induced oxidative stress is the mechanism for a wide range of negative impacts on biota living in radioactively contaminated areas around Chernobyl. The present study tests this hypothesis mechanistically, for... Read More about Low dose ionizing radiation produces too few reactive oxygen species to directly affect antioxidant concentrations in cells.

Differential redox potential between the human cytosolic and mitochondrial branched-chain aminotransferase (2012)
Journal Article

The human branched-chain aminotransferase (hBCAT) isoenzymes are CXXC motif redox sensitive homodimers central to glutamate metabolism in the central nervous system. These proteins respond differently to oxidation by H 2O 2, NO, and S-glutathionylati... Read More about Differential redox potential between the human cytosolic and mitochondrial branched-chain aminotransferase.

Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells (2011)
Journal Article

Aims/hypothesis: This study used proteomics and biochemical approaches to identify novel glucose-regulated proteins and to unveil their role in pancreatic beta cell function. Translationally controlled tumour protein (TCTP) was identified to be one s... Read More about Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells.

Assessment of the influence of different sample processing and cold storage duration on plant free proline content analyses (2010)
Journal Article

Introduction - A method which is widely accepted for the analysis of free proline content in plant tissues is based on the use of 3% sulfosalicylic acid as an extractant, followed by spectrophotometric quantification of a proline-ninhydrin complex in... Read More about Assessment of the influence of different sample processing and cold storage duration on plant free proline content analyses.

A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8 (2010)
Journal Article

Plants are highly adapted to respond to a range of environmental stresses commonly by altering their gene expression and metabolism as a result of cell signalling which may be mediated by reactive oxygen species. The glycine-rich RNA-binding proteins... Read More about A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8.

The effects of glucose ingestion and glucose regulation on memory performance in older adults with mild cognitive impairment (2009)
Journal Article

Background/Objectives: Previous research investigating the impact of glucose ingestion and/or improvements in glucose regulation has found selective cognitive facilitation on episodic memory tasks in successful ageing and dementia. The present study... Read More about The effects of glucose ingestion and glucose regulation on memory performance in older adults with mild cognitive impairment.

S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation (2009)
Journal Article

Specific proteins with reactive thiol(ate) groups are susceptible to nitric oxide (NO) modification, which can result in S-nitrosation, S-thiolation, or disulfide bond formation. In the present study the effect of NO modification on the functionality... Read More about S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation.

S-nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and s-thiolation (2009)
Journal Article

Specific proteins with reactive thiol(ate) groups are susceptible to nitric oxide (NO) modification, which can result in S-nitrosation, S-thiolation, or disulfide bond formation. In the present study the effect of NO modification on the functionality... Read More about S-nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and s-thiolation.

Lipid peroxidation levels in soybean (Glycine max (L.) Merr.) seed parts as a consequence on imbibition stress (2009)
Journal Article

High rainfall and rapid water uptake by dry seed after sowing in the field can result in so-called seed imbibitional damage. Here, lipid peroxidation levels were evaluated in seed testa, embryos and cotyledons of three soybean cultivars (Podravka 95,... Read More about Lipid peroxidation levels in soybean (Glycine max (L.) Merr.) seed parts as a consequence on imbibition stress.

Antioxidative responses in radish (Raphanus sativus L.) plants stressed by copper and lead in nutrient solution and soil (2008)
Journal Article

Radish (Raphanus sativus L.) is commonly grown in urban and suburban areas where the soil may be polluted with heavy metals such as Cu or Pb. In this study, short exposure of radish plantlets to 0.5 mM Cu or Pb in nutrient solution (two days) in grow... Read More about Antioxidative responses in radish (Raphanus sativus L.) plants stressed by copper and lead in nutrient solution and soil.

Implications of dealing with airborne substances and reactive oxygen species: What mammalian lungs, animals, and plants have to say (2007)
Journal Article

A gas-exchange structure interacts with the environment and is constantly challenged by contaminants that may elicit defense responses, thus compromising its primary function. It is also exposed to high concentrations of O2 that can generate reactive... Read More about Implications of dealing with airborne substances and reactive oxygen species: What mammalian lungs, animals, and plants have to say.

Production of reactive oxygen species and reactive nitrogen species by angiosperm stigmas and pollen: Potential signalling crosstalk? (2006)
Journal Article

• Angiosperm stigmas exhibit high levels of peroxidase activity when receptive to pollen. To explore possible function(s) of this peroxidase activity we investigated amounts of reactive oxygen species (ROS), particularly hydrogen peroxide, in stigmas... Read More about Production of reactive oxygen species and reactive nitrogen species by angiosperm stigmas and pollen: Potential signalling crosstalk?.

Ethylene-induced stomatal closure in Arabidopsis occurs via AtrbohF-mediated hydrogen peroxide synthesis (2006)
Journal Article

Ethylene is a plant hormone that regulates many aspects of growth and development. Despite the well-known association between ethylene and stress signalling, its effects on stomatal movements are largely unexplored. Here, genetic and physiological da... Read More about Ethylene-induced stomatal closure in Arabidopsis occurs via AtrbohF-mediated hydrogen peroxide synthesis.

Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis (2005)
Journal Article

Hydrogen peroxide (H2O2) is now recognised as a key signalling molecule in eukaryotes. In plants, H2O2 is involved in regulating stomatal closure, gravitropic responses, gene expression and programmed cell death. Although several kinases, such as oxi... Read More about Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis.

DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines (2004)
Journal Article

This study used cDNA microarray technology to compare gene expression profiles in acute myeloblastic leukaemia (AML) with cDNA dot-blot and real time PCR analysis of cDNA transcripts to confirm array data. Patient AML marrow samples and AML cell line... Read More about DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines.

Hydrogen peroxide is a common signal for darkness and ABA-induced stomatal closure in Pisum sativum L. (2004)
Journal Article

The requirement for hydrogen peroxide (H2O2) generation and action during stomatal closure induced by darkness and abscisic acid (ABA) was investigated in pea (Pisum sativum L.). Stomatal closure induced by darkness or ABA was inhibited by the H2O2-s... Read More about Hydrogen peroxide is a common signal for darkness and ABA-induced stomatal closure in Pisum sativum L..

A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana (2002)
Journal Article

The plant hormone abscisic acid (ABA), synthesized in response to water-deficit stress, induces stomatal closure via activation of complex signaling cascades. Recent work has established that nitric oxide (NO) is a key signaling molecule mediating AB... Read More about A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana.