Electrochemical detection of extracellular hydrogen peroxide in Arabidopsis thaliana: A real-time marker of oxidative stress

Gonz�lez-S�nchez, M. I.; Gonz�lez-Macia, L.; P�rez-Prior, M. T.; Valero, E.; Hancock, J.; Killard, A. J.; Gonzalez-Sanchez, M I; Gonzalez-Macia, Laura; Hancock, John T.; Killard, Anthony

Abstract

An electrochemical approach to directly measure the dynamic process of H2O2 release from cultures of Arabidopsis thaliana cells is reported. This approach is based on H2O2 oxidation on a Pt electrode in conjunction with continuous measurement of sample pH. For [H2O2] 1mm), the amperometric response can be described by Michaelian-type kinetics and a mathematical expression relating current intensity and pH was obtained to quantitatively determine H2O2 concentration. At pH5.5, the detection limit of the sensor was 3.1μm (S/N=3), with a response sensitivity of 0.16Am-1cm-2 and reproducibility was within 6.1% in the range 1-5×10-3m (n=5). Cell suspensions under normal physiological conditions had a pH between 5.5-5.7 and H2O2 concentrations in the range 7.0-20.5μm (n=5). The addition of exogenous H2O2, as well as other potential stress stimuli, was made to the cells and the change in H2O2 concentration was monitored. This real-time quantitative H2O2 analysis is a potential marker for the evaluation of oxidative stress in plant cell cultures. © 2012 Blackwell Publishing Ltd.

• Journal Article Type: Article
• Publication Date: Apr 1, 2013
• Deposit Date: Nov 9, 2012
• Journal: Plant, Cell and Environment
• Print ISSN: 0140-7791
• Electronic ISSN: 1365-3040
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 36
• Issue: 4
• Pages: 869-878
• DOI: https://doi.org/10.1111/pce.12023
• Keywords: hydrogen peroxide, plant cells, oxidative stress
• Public URL: https://uwe-repository.worktribe.com/output/933184
• Publisher URL: http://dx.doi.org/10.1111/pce.12023
• Contract Date: Jun 4, 2016