Leaf arginine spraying improves leaf gas exchange under water deficit and root antioxidant responses during the recovery period

Silveira, N. M.; Ribeiro, R. V.; de Morais, S. F. N.; de Souza, S. C. R.; da Silva, S. F.; Seabra, A. B.; Hancock, J.T.; Machado, E. C.

Abstract

© 2021 Elsevier Masson SAS Arginine (Arg) metabolism is associated with many cellular and developmental processes in plants and proline, nitric oxide (NO) and polyamines (PAs) have a wide range of physiological functions in plants, including increased tolerance to environmental stresses. This study aimed to test the hypothesis that Arg spraying would stimulate the synthesis of proline, NO and PAs, reducing the oxidative damage caused by water deficit (WD) and increasing drought tolerance of sugarcane plants. Sugarcane plants were sprayed with water or Arg 1 mM, and subjected to WD by gradual addition of polyethylene glycol (PEG-8000) to the nutrient solution. As references, sugarcane plants were grown in nutrient solution without PEG-8000 and sprayed or not with Arg. Our data indicate that exogenous Arg supply improved leaf gas exchange during water deficit and enhanced the root antioxidative protection of sugarcane plants during the recovery period. Arg supply prevented the proline accumulation induced by water deficit and then the main pathway for proline synthesis is likely through glutamate instead of arginine. Although Arg is a substrate for NO and PAs production, supplying Arg had only slight effects in both NO and PAs levels. The spraying of amino acids capable of reducing the harmful effects of drought, such as Arg, can be an alternative to improve crop growth under field conditions.

Citation

Silveira, N. M., Ribeiro, R. V., de Morais, S. F. N., de Souza, S. C. R., da Silva, S. F., Seabra, A. B., …Machado, E. C. (2021). Leaf arginine spraying improves leaf gas exchange under water deficit and root antioxidant responses during the recovery period. Plant Physiology and Biochemistry, 162, 315-326. https://doi.org/10.1016/j.plaphy.2021.02.036

• Journal Article Type: Article
• Acceptance Date: Feb 23, 2021
• Online Publication Date: Mar 3, 2021
• Publication Date: May 1, 2021
• Deposit Date: Feb 24, 2021
• Publicly Available Date: Mar 4, 2022
• Journal: Plant Physiology and Biochemistry
• Print ISSN: 0981-9428
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 162
• Pages: 315-326
• DOI: https://doi.org/10.1016/j.plaphy.2021.02.036
• Public URL: https://uwe-repository.worktribe.com/output/7109650