Skip to main content

Research Repository

Advanced Search

Hydrogen peroxide is a common signal for darkness and ABA-induced stomatal closure in Pisum sativum L.

Desikan, Radhika; Cheung, Man-Kim; Clarke, Andrew; Golding, Sarah; Sagi, Moshe; Fluhr, Robert; Rock, Christopher; Hancock, John T.; Neill, Steven


Radhika Desikan

Man-Kim Cheung

Andrew Clarke

Sarah Golding

Moshe Sagi

Robert Fluhr

Christopher Rock

Profile Image

John Hancock
Professor in Cell Signalling


The requirement for hydrogen peroxide (H2O2) generation and action during stomatal closure induced by darkness and abscisic acid (ABA) was investigated in pea (Pisum sativum L.). Stomatal closure induced by darkness or ABA was inhibited by the H2O2-scavenging enzyme catalase or the antioxidant N-acetyl cysteine (NAC), or by diphenylene iodonium (DPI), an inhibitor of the H2O2-generating enzyme NADPH oxidase. Exogenous H2O2 induced stomatal closure in a dose- and time-dependent manner, and H2O2 was also required for ABA-inhibition of stomatal opening in the light. H2O2 accumulation in guard cells was increased by darkness or ABA, as assessed with the fluorescent dye dichlorodihydrofluorescein diacetate (H2-DCFDA) and confocal microscopy. Such increases were inhibited by catalase, NAC or DPI, consistent with the effects of these compounds on stomatal apertures. Employing polymerase chain reaction (PCR) with degenerate oligonucleotide primers, several NADPH oxidase homologues were identified from pea genomic DNA that had substantial identity to the Arabidopsis thaliana (L.) Heynh. rboh (respiratory burst oxidase homologue) genes. Furthermore, an antibody raised against the tomato rboh identified immunoreactive proteins in epidermal, mesophyll and guard cells.


Desikan, R., Cheung, M., Clarke, A., Golding, S., Sagi, M., Fluhr, R., …Neill, S. (2004). Hydrogen peroxide is a common signal for darkness and ABA-induced stomatal closure in Pisum sativum L. Functional Plant Biology, 31(9), 913-920

Journal Article Type Article
Publication Date Jan 1, 2004
Journal Functional Plant Biology
Print ISSN 1445-4408
Publisher CSIRO Publishing
Peer Reviewed Peer Reviewed
Volume 31
Issue 9
Pages 913-920
Public URL