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ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells

Desikan, Radhika; Cheung, Man-Kim; Bright, Jo; Henson, Dan; Hancock, John T.; Neill, Steven


Radhika Desikan

Man-Kim Cheung

Jo Bright

Dan Henson

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John Hancock
Professor in Cell Signalling


Increased synthesis and redistribution of the phytohormone abscisic acid (ABA) in response to water deficit stress initiates an intricate network of signalling pathways in guard cells leading to stomatal closure. Despite the large number of ABA signalling intermediates that are known in guard cells, new discoveries are still being made. Recently, the reactive oxygen species hydrogen peroxide (H2O2) and the reactive nitrogen species nitric oxide (NO) have been identified as key molecules regulating ABA-induced stomatal closure in various species. As with many other physiological responses in which H2O2 and NO are involved, stomatal closure in response to ABA also appears to require the tandem synthesis and action of both these signalling molecules. Recent pharmacological and genetic data have identified NADPH oxidase as a source of H2O2, whilst nitrate reductase has been identified as a source of NO in Arabidopsis guard cells. Some signalling components positioned downstream of H2O2 and NO are calcium, protein kinases and cyclic GMP. However, the exact interaction between the various signalling components in response to H2O2 and NO in guard cells remains to be established.


Desikan, R., Cheung, M., Bright, J., Henson, D., Hancock, J. T., & Neill, S. (2004). ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells. Journal of Experimental Botany, 55(395), 205-212

Journal Article Type Article
Publication Date Jan 1, 2004
Journal Journal of Experimental Botany
Print ISSN 0022-0957
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 55
Issue 395
Pages 205-212
Keywords abscisic acid, guard cells, hydrogen peroxide, nitric oxide, signalling
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