DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines

Court, Emma Louise; Smith, M. Ann; Avent, Neil David; Morgan, Lyn M.; Gray, Atherton G.; Hancock, John T.; Smith, J. Graham

Abstract

This study used cDNA microarray technology to compare gene expression profiles in acute myeloblastic leukaemia (AML) with cDNA dot-blot and real time PCR analysis of cDNA transcripts to confirm array data. Patient AML marrow samples and AML cell lines were compared with normal/non-AML samples. Screening revealed five particular genes to be significantly differentially expressed across the sample groups. The migration-inhibitory factor-related-proteins 8 and 14 (MRP-8 and MRP-14) genes, the products of which inhibit cell migration and differentiation were the most highly expressed in non-malignant cells. The high-mobility-group-protein gene (HMG-1) was up regulated in leukaemic samples and cell lines, which may be associated with aggressive disease. Also upregulated in malignant samples were genes encoding c-myc and glutathione-S-transferase pi (GSTP), the latter implicated in chemotherapy resistance. Faulty expression of such genes may contribute to the pathogenesis of AML and resistance to treatment. © 2004 Elsevier Ltd. All rights reserved.

• Journal Article Type: Article
• Publication Date: Jul 1, 2004
• Journal: Leukemia Research
• Print ISSN: 0145-2126
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 28
• Issue: 7
• Pages: 743-753
• DOI: https://doi.org/10.1016/j.leukres.2003.11.011
• Keywords: acute myeloblastic leukaemia (AML), cDNA microarrays, glutathione-S-transferase pi (GSTP), c-myc, migration-inhibitory factor-related protein (MRP-8 and MRP-14), high-mobility-group-protein (HMG-1)
• Public URL: https://uwe-repository.worktribe.com/output/1068867
• Publisher URL: http://dx.doi.org/10.1016/j.leukres.2003.11.011