Emma Louise Court
DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines
Court, Emma Louise; Smith, M. Ann; Avent, Neil David; Morgan, Lyn M.; Gray, Atherton G.; Hancock, John T.; Smith, J. Graham
Authors
M. Ann Smith
Neil David Avent
Lyn M. Morgan
Atherton G. Gray
John Hancock John.Hancock@uwe.ac.uk
Professor in Cell Signalling
J. Graham Smith
Abstract
This study used cDNA microarray technology to compare gene expression profiles in acute myeloblastic leukaemia (AML) with cDNA dot-blot and real time PCR analysis of cDNA transcripts to confirm array data. Patient AML marrow samples and AML cell lines were compared with normal/non-AML samples. Screening revealed five particular genes to be significantly differentially expressed across the sample groups. The migration-inhibitory factor-related-proteins 8 and 14 (MRP-8 and MRP-14) genes, the products of which inhibit cell migration and differentiation were the most highly expressed in non-malignant cells. The high-mobility-group-protein gene (HMG-1) was up regulated in leukaemic samples and cell lines, which may be associated with aggressive disease. Also upregulated in malignant samples were genes encoding c-myc and glutathione-S-transferase pi (GSTP), the latter implicated in chemotherapy resistance. Faulty expression of such genes may contribute to the pathogenesis of AML and resistance to treatment. © 2004 Elsevier Ltd. All rights reserved.
Citation
Gray, A. G., Morgan, L. M., Avent, N. D., Smith, M. A., Court, E. L., Hancock, J. T., & Smith, J. G. (2004). DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines. Leukemia Research, 28(7), 743-753. https://doi.org/10.1016/j.leukres.2003.11.011
Journal Article Type | Article |
---|---|
Publication Date | Jul 1, 2004 |
Journal | Leukemia Research |
Print ISSN | 0145-2126 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 28 |
Issue | 7 |
Pages | 743-753 |
DOI | https://doi.org/10.1016/j.leukres.2003.11.011 |
Keywords | acute myeloblastic leukaemia (AML), cDNA microarrays, glutathione-S-transferase pi (GSTP), c-myc, migration-inhibitory factor-related protein (MRP-8 and MRP-14), high-mobility-group-protein (HMG-1) |
Public URL | https://uwe-repository.worktribe.com/output/1068867 |
Publisher URL | http://dx.doi.org/10.1016/j.leukres.2003.11.011 |
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