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Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids (2023)
Journal Article
Smart, A., Crew, A., Doran, O., & Hart, J. (2023). Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids. Journal of Food Composition and Analysis, 122, Article 105488. https://doi.org/10.1016/j.jfca.2023.105488

This paper describes, for the first time, the development of a novel trilinolein biosensor based on the immobilisation of lipase and lipoxygenase (LOX) onto a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) in conjunction with amper... Read More about Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids.

Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes (2020)
Journal Article
Crew, A., Karamatic Crew, V., Bagelow, L., Walser, P., Thornton, N., & Hart, J. (2020). Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes. Vox Sanguinis, 115(S1), 271-271. https://doi.org/10.1111/vox.13031

Background: The five antigens of the Yt blood group system are located on the membrane GPI‐linked erythrocyte acetylcholinesterase (AChE‐E), which is implicated in a range of clinical issues, including sleep apnoea and neurotoxin poisoning. Conformat... Read More about Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes.

Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids (2020)
Journal Article
Smart, A., Hart, J., Crew, A., & Doran, O. (2020). Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids. Applied Sciences, 10(21), 1-13. https://doi.org/10.3390/app10217779

This paper describes the design, development and characterisation of an electrochemical biosensor for the measurement of linoleic and α-linolenic acid, as representative free polyunsaturated fatty acids (PUFAs), that may be implicated in food safety... Read More about Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids.

Screen-printed carbon based biosensors and their applications in agri-food safety (2020)
Journal Article
Smart, A., Crew, A., Pemberton, R., Hughes, G., Doran, O., & Hart, J. P. (2020). Screen-printed carbon based biosensors and their applications in agri-food safety. Trends in Analytical Chemistry, 127, Article 115898. https://doi.org/10.1016/j.trac.2020.115898

This review focuses on the ways in which screen-printed carbon electrodes have been tailored with different biorecognition elements, including enzymes, antibodies, and aptamers, often with other modifiers, such as mediators and nanoparticles, to prod... Read More about Screen-printed carbon based biosensors and their applications in agri-food safety.

Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method (2019)
Journal Article
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2020). Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method. Biosensors and Bioelectronics, 150, Article 111837. https://doi.org/10.1016/j.bios.2019.111837

© 2019 Elsevier B.V. This is the first report on the fabrication, characterisation and application of an electrochemical (bio)sensor system for the simultaneous measurement of skatole and androstenone. A biosensor for androstenone was fabricated usin... Read More about Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method.

An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements (2019)
Journal Article
Smart, A., Westmacott, K. L., Crew, A., Doran, O., & Hart, J. P. (2019). An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements. Biosensors, 9(3), Article 98. https://doi.org/10.3390/bios9030098

An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its elect... Read More about An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements.

Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour (2018)
Journal Article
Hughes, G., Pemberton, R. . M., Nicholas, P., & Hart, J. P. (2018). Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour. Electroanalysis, 30(8), 1608-1612. https://doi.org/10.1002/elan.+201+800+104

This paper describes a simple, convenient approach to the fabrication of microband electrodes and microband biosensors based on screen printing technology. These devices were printed in a three-electrode configuration on one strip; a silver/silver ch... Read More about Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour.

A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors (2017)
Journal Article
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2018). A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors. Talanta, 181, 13-18. https://doi.org/10.1016/j.talanta.2017.12.074

© 2017 Elsevier B.V. This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B1 (thiamine), B2 (r... Read More about A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors.

Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode (2017)
Journal Article
Nicholas, P., Pittson, R., & Hart, J. P. (2018). Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode. Food Chemistry, 241, 122-126. https://doi.org/10.1016/j.foodchem.2017.08.077

© 2017 Elsevier Ltd This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that th... Read More about Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode.

Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL (2017)
Journal Article
McDowall, J. S., Ntai, I., Honeychurch, K. C., Hart, J. P., Colin, P., Schneider, B. L., & Brown, D. R. (2017). Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL. Molecular and Cellular Neuroscience, 85, 1-11. https://doi.org/10.1016/j.mcn.2017.08.001

© 2017 Elsevier Inc. The normal cellular role of α-synuclein is of potential importance in understanding diseases in which an aggregated form of the protein has been implicated. A potential loss or change in the normal function of α-synuclein could p... Read More about Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL.

Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses (2016)
Journal Article
Pemberton, R. M., Crew, A., Hughes, G., Westmacott, K., Honeychurch, K. C., Crew, A. P., …Hart, J. P. (2016). Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses. Biosensors, 6(4), 50. https://doi.org/10.3390/bios6040050

This review describes recent advances in the fabrication of electrochemical (bio)sensors based on screen-printing technology involving carbon materials and their application in biomedical, agri-food and environmental analyses. It will focus on the va... Read More about Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses.

Voltammetric behaviour and determination of clonazepam using a disposable screen-printed sensor and its determination in serum and wine (2016)
Presentation / Conference
Honeychurch, K. C., Brooks, J., & Hart, J. P. (2016, August). Voltammetric behaviour and determination of clonazepam using a disposable screen-printed sensor and its determination in serum and wine. Presented at Electrochem 2016, Leicester, UK

Clonazepam Screen-printed Carbon Electrodes Cyclic Voltammetry Gas chromatography mass spectrometry Adsorptive Stripping Voltammetry Sample Preparation and Analysis Conclusions

Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation (2016)
Journal Article
Hart, J. P., Liébana, S., Jones, L. J., Drago, G. A., Pittson, R. W., Liu, D., …Hart, J. P. (2016). Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation. Sensors and Actuators B: Chemical, 231, 384-392. https://doi.org/10.1016/j.snb.2016.02.142

© 2016 Elsevier B.V. All rights reserved. A new generic platform for the development of microbiosensors combining screen-printing and ultrafast pulsed laser technologies has been developed, characterised and evaluated. This new platform consists of a... Read More about Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation.

A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring (2016)
Journal Article
Hart, J. P., Pemberton, R. M., Hughes, G., Hughes, G., Pemberton, R., Fielden, P. R., & Hart, J. P. (2016). A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring. Analytica Chimica Acta, 933, 82-88. https://doi.org/10.1016/j.aca.2016.06.005

© 2016 Elsevier B.V. A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printe... Read More about A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring.

Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications (2016)
Journal Article
Jackson, S. K., Pemberton, R. M., Kanyong, P., Hughes, G., Pemberton, R., Jackson, S., & Hart, J. P. (2016). Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications. Analytical Letters, 49(2), 236-244. https://doi.org/10.1080/00032719.2015.1070166

© 2016, Copyright © Taylor & Francis Group, LLC. ABSTRACT: This paper reports the development and application of a biosensor for the amperometric determination of galactose in the presence of human hepatocellular carcinoma cells with and without a... Read More about Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications.

Sensor and method for detecting androstenone or skatole in boar taint (2016)
Patent
Hart, J., Crew, A., McGuire, N., & Doran, O. (2016). Sensor and method for detecting androstenone or skatole in boar taint. EP2966441 A1

The present application is concerned with a sensor system for and a method of detecting, and preferably quantifying, androstenone (CAS Reg.No. 18339-16-7) and/or skatole (CAS Reg. No. 83-34-1), the chemicals associated with boar taint. In a preferred... Read More about Sensor and method for detecting androstenone or skatole in boar taint.

The design, development and application of electrochemical glutamate biosensors (2015)
Journal Article
Hart, J. P., Pemberton, R. M., Hughes, G., Hughes, G., Pemberton, R., Fielden, P. R., & Hart, J. P. (2016). The design, development and application of electrochemical glutamate biosensors. Trends in Analytical Chemistry, 79, 106-113. https://doi.org/10.1016/j.trac.2015.10.020

© 2015 Elsevier B.V. The development of biosensors for the determination of glutamate has been of great research interest for the past 25 years due to its importance in biomedical and food studies. This review focusses on the various strategies used... Read More about The design, development and application of electrochemical glutamate biosensors.

Development of a voltammetric assay, using screen-printed electrodes, for clonazepam and its application to beverage and serum samples (2015)
Journal Article
Honeychurch, K. C., Brooks, J., & Hart, J. P. (2016). Development of a voltammetric assay, using screen-printed electrodes, for clonazepam and its application to beverage and serum samples. Talanta, 147, 510-515. https://doi.org/10.1016/j.talanta.2015.10.032

© 2015 Elsevier B.V. All rights reserved. This paper describes the development of an electrochemical assay based on screen-printed carbon sensors for the determination of clonazepam in serum and in wine. The cyclic voltammetric behaviour of the drug... Read More about Development of a voltammetric assay, using screen-printed electrodes, for clonazepam and its application to beverage and serum samples.

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications (2015)
Journal Article
Hughes, G., Pemberton, R. M., Fielden, P. R., & Hart, J. P. (2015). Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications. Sensors and Actuators B: Chemical, 216, 614-621. https://doi.org/10.1016/j.snb.2015.04.066

© 2015 Elsevier B.V. Abstract A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB) has been investigated as the base transducer for a reagentless glutamate biosensor. The biopolymer chitosan (CHIT) and multiwall... Read More about Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications.

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers (2014)
Journal Article
Sage, I. C., Drago, G. A., Pemberton, R., Cox, T., Tuffin, R., Griffiths, J., …Hart, J. P. (2014). Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers. Sensors, 14(11), 20519-20532. https://doi.org/10.3390/s141120519

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of... Read More about Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers.

Novel reductive-reductive mode electrochemical detection of Rohypnol following liquid chromatography and its determination in coffee (2014)
Journal Article
Honeychurch, K. C., Davidson, G. M., Brown, E., & Hart, J. P. (2015). Novel reductive-reductive mode electrochemical detection of Rohypnol following liquid chromatography and its determination in coffee. Analytica Chimica Acta, 853, 222-227. https://doi.org/10.1016/j.aca.2014.09.033

© 2014 Elsevier B.V. Rohypnol (flunitrazepam) has been successfully determined in coffee by high performance liquid chromatography dual electrode detection (LC-DED) in the dual reductive mode. Initial studies were performed to optimise the chromatogr... Read More about Novel reductive-reductive mode electrochemical detection of Rohypnol following liquid chromatography and its determination in coffee.

Development of a disposable screen-printed amperometric biosensor based on glutamate dehydrogenase, for the determination of glutamate in clinical and food applications (2014)
Journal Article
Hughes, G., Pemberton, R., Fielden, P., & Hart, J. P. (2014). Development of a disposable screen-printed amperometric biosensor based on glutamate dehydrogenase, for the determination of glutamate in clinical and food applications. Analytical & Bioanalytical Electrochemistry, 6(4), 435-449

A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola’s Blue (MB) has been investigated as the base transducer for a glutamate biosensor. The sandwich biosensor was fabricated by firstly depositing a chitosan (CHIT) layer... Read More about Development of a disposable screen-printed amperometric biosensor based on glutamate dehydrogenase, for the determination of glutamate in clinical and food applications.

Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations (2014)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2014). Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations. https://doi.org/10.5640/insc.040101

Benzodiazepines are an important class of drugs commonly administered with a potential for abuse and environmental pollution. This review focuses on the liquid chromatographic electrochemical detection of the benzodiazepine class of drugs. These are... Read More about Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations.

The redox behaviour of diazepam (valium) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay (2013)
Presentation / Conference
Honeychurch, K. C., Crew, A. P., Northall, H., Radbourne, S., Davies, O., Newman, S., & Hart, J. P. (2013, June). The redox behaviour of diazepam (valium) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay. Presented at ISE Satellite Student Regional Symposium: Great Western Electrochemistry Meeting, University of Bath, Bath, England

Use of whole blood for analysis of disease-associated biomarkers (2013)
Journal Article
McLeod, J., Pemberton, R. M., May, J. E., Pemberton, R., Hart, J. P., McLeod, J. D., …Doran, O. (2013). Use of whole blood for analysis of disease-associated biomarkers. Analytical Biochemistry, 437(1), 59-61. https://doi.org/10.1016/j.ab.2013.02.024

Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of... Read More about Use of whole blood for analysis of disease-associated biomarkers.

Microfabricated glucose biosensor for culture welloperation (2013)
Journal Article
Hart, J. P., Luxton, R., Xu, J., Griffiths, J., Sage, I., Tuffin, R., …Hart, J. P. (2013). Microfabricated glucose biosensor for culture welloperation. Biosensors and Bioelectronics, 42(1), 668-677. https://doi.org/10.1016/j.bios.2012.11.032

A water-based carbon screen-printing ink formulation, containing the redox mediator cobalt phthalocyanine (CoPC) and the enzyme glucose oxidase (GOx), was investigated for its suitability to fabricate glucose microbiosensors in a 96-well microplate f... Read More about Microfabricated glucose biosensor for culture welloperation.

Development of an amperometric screen-printed galactose biosensor for serum analysis (2013)
Journal Article
Pemberton, R. M., Kanyong, P., Pemberton, R., Jackson, S. K., & Hart, J. P. (2013). Development of an amperometric screen-printed galactose biosensor for serum analysis. Analytical Biochemistry, 435(2), 114-119. https://doi.org/10.1016/j.ab.2013.01.006

The development of a disposable amperometric biosensor for the measurement of circulating galactose in serum is described. The biosensor comprises a screen-printed carbon electrode (SPCE), incorporating the electrocatalyst cobalt phthalocyanine (CoPC... Read More about Development of an amperometric screen-printed galactose biosensor for serum analysis.

The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay (2013)
Journal Article
Crew, A., Honeychurch, K. C., Crew, A. P., Northall, H., Radbourne, S., Davies, O., …Hart, J. P. (2013). The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay. Talanta, 116, 300-307. https://doi.org/10.1016/j.talanta.2013.05.017

In this study we investigated the possibility of applying disposable electrochemical screen-printed carbon sensors for the rapid identification and quantitative determination of diazepam in beverages. This was achieved utilising a previously unreport... Read More about The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay.

The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine (2012)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2012). The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine. Advances in Analytical Chemistry, 2(5), 46-52. https://doi.org/10.5923/j.aac.20120205.01

Screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) have been successfully used for the manufacture of microband electrodes for the determination of reduced glutathione (GSH). Initially investigati... Read More about The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine.

Voltammetric behaviour of lead at a microband screen-printed carbon electrode and its trace determination in plants by stripping voltammetry (2012)
Presentation / Conference
Phillips, R., Honeychurch, K. C., Crew, A. P., Davies, R., Willey, N., & Hart, J. P. (2012, September). Voltammetric behaviour of lead at a microband screen-printed carbon electrode and its trace determination in plants by stripping voltammetry. Poster presented at Electrochem 2012: Electrochemical Horizons, Trinity College, Dublin

Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis (2012)
Journal Article
Kanyong, P., Pemberton, R., Jackson, S. K., & Hart, J. P. (2012). Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis. Analytical Biochemistry, 428(1), 39-43. https://doi.org/10.1016/j.ab.2012.05.027

A screen-printed carbon electrode (SPCE) incorporating the electrocatalyst cobalt phthalocyanine (CoPC), fabricated using a water-based ink formulation, has been investigated as the base transducer for a uric acid biosensor. A sandwich biosensor was... Read More about Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.

Redox mechanisms of rohypnol and mogadon and their exploitation in an assay using dual electrode ECD following HPLC (2012)
Presentation / Conference
Honeychurch, K. C., Li, H., Pericleous, S., Antoniou, M., Abed, A., & Hart, J. P. (2012, June). Redox mechanisms of rohypnol and mogadon and their exploitation in an assay using dual electrode ECD following HPLC. Presented at Great Western Electrochemistry Meeting, University of Bath

Importance of the 1,4-benzodiazepine class of drugs Cyclic voltammetric behaviour of Flunitrazepam (Rohypnol), and Nitrazepam (Mogadon) Possible mechanism for their voltammetric behaviour Optimisation of Liquid Chromatographic Separation and... Read More about Redox mechanisms of rohypnol and mogadon and their exploitation in an assay using dual electrode ECD following HPLC.

Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum (2012)
Journal Article
Honeychurch, K. C., Chong, A. T., Elamin, K., & Hart, J. P. (2012). Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum. Analytical Methods, 4(1), 132-140. https://doi.org/10.1039/c1ay05419h

Previously unreported voltammetric redox behaviour was identified for flunitrazepam, lorazepam and diazepam at a glassy carbon electrode. This was successfully exploited for their determination in serum by high performance liquid chromatography dual... Read More about Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum.

Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples (2011)
Journal Article
Jenkins, A. T., Gilbert, L., Browning, S., & Hart, J. P. (2011). Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples. Sensors and Actuators B: Chemical, 160(1), 1322-1327. https://doi.org/10.1016/j.snb.2011.09.069

An amperometric phosphate biosensor, based on a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) is described. The immobilisation of the enzyme pyruvate oxidase (PyOd) was investigated using pre-formed cellulose acetate/cellulose nit... Read More about Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples.

Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection (2011)
Journal Article
Kumar, A., Hart, J. P., & McCalley, D. V. (2011). Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection. Journal of Chromatography A, 1218(25), 3854-3861. https://doi.org/10.1016/j.chroma.2011.04.034

The determination of catecholamines in urine was investigated using hydrophilic interaction chromatography (HILIC) as an alternative to the commonly used reversed-phase (RP) method. A number of different approaches were explored, including per-aqueou... Read More about Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection.

The voltammetric behaviour of lead at a microband screen-printed carbon electrode and its determination in acetate leachates from glazed ceramic plates (2011)
Journal Article
Honeychurch, K. C., Al-Berezanchi, S., & Hart, J. P. (2011). The voltammetric behaviour of lead at a microband screen-printed carbon electrode and its determination in acetate leachates from glazed ceramic plates. Talanta, 84(3), 717-723. https://doi.org/10.1016/j.talanta.2011.01.073

Microband screen-printed carbon electrodes (μBSPCEs) without further modification have been investigated as disposable sensors for the measurement of lead in acetate leachates from ceramic glazed plates. Cyclic voltammetry was employed to elucidate t... Read More about The voltammetric behaviour of lead at a microband screen-printed carbon electrode and its determination in acetate leachates from glazed ceramic plates.

A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides (2011)
Journal Article
Crew, A. P., Lonsdale, D., Byrd, N., Pittson, R., & Hart, J. P. (2011). A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides. Biosensors and Bioelectronics, 26(6), 2847-2851. https://doi.org/10.1016/j.bios.2010.11.018

Organophosphate pesticides present serious risks to human and environmental health. A rapid reliable, economical and portable analytical system will be of great benefit in the detection and prevention of contamination. A biosensor array based on six... Read More about A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides.

A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture (2011)
Journal Article
Xu, J., Pemberton, R., Pittson, R., Drago, G. A., Griffiths, J., Jackson, S. K., & Hart, J. P. (2011). A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture. Biosensors and Bioelectronics, 26(5), 2448-2453. https://doi.org/10.1016/j.bios.2010.10.030

Microband biosensors, screen-printed from a water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, were used to monitor glucose levels continuously in buffer and culture medium. Five biosensors were o... Read More about A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture.

A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture (2011)
Journal Article
Pemberton, R., Xu, J., Pittson, R., Drago, G., Griffiths, J., Jackson, S. K., & Hart, J. P. (2011). A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture. Biosensors and Bioelectronics, 26(5), 2448-2453. https://doi.org/10.1016/j.bios.2010.10.030

#Microband biosensors, screen-printed from a water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, were used to monitor glucose levels continuously in buffer and culture medium. Five biosensors were... Read More about A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture.

Voltammetric behavior of DNA and its derivatives using screen printed carbon electrodes and its possible application in genotoxicity screening (2010)
Journal Article
Rawson, F. J., Jackson, S. K., & Hart, J. P. (2010). Voltammetric behavior of DNA and its derivatives using screen printed carbon electrodes and its possible application in genotoxicity screening. Analytical Letters, 43(10-11), 1790-1800. https://doi.org/10.1080/00032711003653908

The aim of the current work was to characterize the electrochemical behavior of DNA and its derivatives using screen printed carbon electrodes (SPCEs). Differential pulse voltammetry (DPV) was carried out on guanine, adenine, 8-oxyguanine, 8-deoxygau... Read More about Voltammetric behavior of DNA and its derivatives using screen printed carbon electrodes and its possible application in genotoxicity screening.

Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+ (2010)
Journal Article
Piano, M., Serban, S., Pittson, R., Drago, G. A., & Hart, J. P. (2010). Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+. Talanta, 82(1), 34-37. https://doi.org/10.1016/j.talanta.2010.03.051

A biosensor for the measurement of lactate in serum has been developed, which is based on a screen-printed carbon electrode, modified with Meldola's Blue-Reinecke Salt (MBRS-SPCE), coated with the enzyme lactate dehydrogenase NAD+ dependent (from Por... Read More about Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+.

Cyclic voltammetric behaviour of Rohypnol, Ativan and Valium and its exploitation for their determination in serum using a novel method involving liquid chromatography-dual electrode detection (2010)
Presentation / Conference
Honeychurch, K. C., Chong, A., Elamin, K., & Hart, J. P. (2010, June). Cyclic voltammetric behaviour of Rohypnol, Ativan and Valium and its exploitation for their determination in serum using a novel method involving liquid chromatography-dual electrode detection. Paper presented at Great Western Electrochemistry Meeting 2010, University of Bath, Bath, UK

Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine- modified screen-printed carbon electrode and its application in pharmaceutical and food analysis (2010)
Journal Article
Honeychurch, K. C., Gilbert, L., & Hart, J. P. (2010). Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine- modified screen-printed carbon electrode and its application in pharmaceutical and food analysis. Analytical and Bioanalytical Chemistry, 396(8), 3103-3111. https://doi.org/10.1007/s00216-010-3534-x

Cobalt phthalocyanine-modified screen-printed carbon electrodes (CoPC-SPCEs) have been investigated as disposable sensors for the measurement of citric acid. The analyte was found to undergo an electrocatalytic oxidation process involving the Co2+/Co... Read More about Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine- modified screen-printed carbon electrode and its application in pharmaceutical and food analysis.

Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins (2010)
Journal Article
Pemberton, R., Rawson, F. J., Xu, J., Pittson, R., Drago, G. A., Griffiths, J., …Hart, J. P. (2010). Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins. Microchimica Acta, 170(3), 321-330. https://doi.org/10.1007/s00604-010-0326-0

Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferro... Read More about Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins.

Studies towards an amperometric phosphate ion biosensor for urine and water analysis (2010)
Journal Article
Jenkins, A. T., Gilbert, L., Browning, S., & Hart, J. P. (2010). Studies towards an amperometric phosphate ion biosensor for urine and water analysis. Microchimica Acta, 170(3), 331-336. https://doi.org/10.1007/s00604-010-0316-2

An amperometric biosensor for phosphate ion is described that is based on a cobalt phthalocyanine modified screen-printed carbon electrode (CoPC-SPCE). The biosensor operation is based on the enzyme pyruvate oxidase (PyOd) which catalyses the oxidati... Read More about Studies towards an amperometric phosphate ion biosensor for urine and water analysis.

A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose (2010)
Journal Article
Piano, M., Serban, S., Biddle, N., Pittson, R., Drago, G. A., & Hart, J. P. (2010). A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose. Analytical Biochemistry, 396(2), 269-274. https://doi.org/10.1016/j.ab.2009.09.028

A biosensor for the measurement of glucose in serum has been developed, based on a screen-printed carbon electrode modified with Meldola's Blue-Reinecke salt, coated with the enzyme glucose dehydrogenase (from Bacillus sp.), and nicotinamide adenine... Read More about A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose.

Development of an amperometric assay for phosphate ions in urine based on a chemically modified screen-printed carbon electrode (2009)
Journal Article
Gilbert, L., Jenkins, A. T., Browning, S., & Hart, J. P. (2009). Development of an amperometric assay for phosphate ions in urine based on a chemically modified screen-printed carbon electrode. Analytical Biochemistry, 393(2), 242-247. https://doi.org/10.1016/j.ab.2009.06.038

An amperometric assay for the determination of inorganic phosphate (Pi) in urine has been developed without the need for sample preparation. A screen-printed carbon electrode modified with the electrocatalyst cobalt phthalocyanine (CoPC-SPCE) and cov... Read More about Development of an amperometric assay for phosphate ions in urine based on a chemically modified screen-printed carbon electrode.

The electrochemical behavior of nitrazepam at a screen-printed carbon electrode and its determination in beverages by adsorptive stripping voltammetry (2009)
Journal Article
McGuire, N. D., Honeychurch, K. C., & Hart, J. P. (2009). The electrochemical behavior of nitrazepam at a screen-printed carbon electrode and its determination in beverages by adsorptive stripping voltammetry. Electroanalysis, 21(19), 2165-2170. https://doi.org/10.1002/elan.200904667

The cyclic voltammetric behavior of nitrazepam was investigated at screen-printed carbon electrodes over the range1.5 V to {thorn}1.5 V. Two reduction peaks were observable on the negative scan, at 0.7 V, and 1.2 V using pH 6 buffer. On the return sc... Read More about The electrochemical behavior of nitrazepam at a screen-printed carbon electrode and its determination in beverages by adsorptive stripping voltammetry.

Application of screen-printed microband biosensors to end-point measurements of glucose and cell numbers in HepG2 cell culture (2009)
Journal Article
Jackson, S. K., Pemberton, R., Xu, J., Pittson, R., Biddle, N., Drago, G. A., & Hart, J. P. (2009). Application of screen-printed microband biosensors to end-point measurements of glucose and cell numbers in HepG2 cell culture. Analytical Biochemistry, 385(2), 334-341. https://doi.org/10.1016/j.ab.2008.10.037

Microband glucose biosensors were produced by insulating and sectioning through a screen-printed, water-based carbon electrode containing cobalt phthalocyanine redox mediator and glucose oxidase enzyme. Under quiescent conditions at 37 °C, at an oper... Read More about Application of screen-printed microband biosensors to end-point measurements of glucose and cell numbers in HepG2 cell culture.

A microband lactate biosensor fabricated using a water-based screen-printed carbon ink (2009)
Journal Article
Rawson, F. J., Purcell, W. M., Xu, J., Pemberton, R., Fielden, P. R., Biddle, N., & Hart, J. P. (2009). A microband lactate biosensor fabricated using a water-based screen-printed carbon ink. Talanta, 77(3), 1149-1154. https://doi.org/10.1016/j.talanta.2008.08.020

The present study demonstrated for the first time that screen-printed carbon microband electrodes fabricated from water-based ink can readily detect H2O2 and that the same ink, with the addition of lactate oxidase, can be used to construct microband... Read More about A microband lactate biosensor fabricated using a water-based screen-printed carbon ink.

Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis (2009)
Journal Article
Pemberton, R., Pittson, R., Biddle, N., & Hart, J. P. (2009). Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis. Biosensors and Bioelectronics, 24(5), 1246-1252. https://doi.org/10.1016/j.bios.2008.07.035

Microband glucose biosensors were fabricated by screen-printing a water-based carbon ink formulation containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, then insulating and sectioning through the thick (20 μm) film to ex... Read More about Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis.

Part II, Ch.6, Preparation of screen-printed electrochemical immunosensors for estradiol, and their application in biological fluids (2009)
Book Chapter
Pemberton, R., & Hart, J. P. (2009). Part II, Ch.6, Preparation of screen-printed electrochemical immunosensors for estradiol, and their application in biological fluids. In A. Rasooly, & K. E. Herold (Eds.), Biosensors and Biodetection Methods and Protocols. Vol.2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors. Humana Press Inc., USA

Determination of flunitrazepam and nitrazepam in beverage samples by liquid chromatography with dual electrode detection using a carbon fibre veil electrode (2008)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2008). Determination of flunitrazepam and nitrazepam in beverage samples by liquid chromatography with dual electrode detection using a carbon fibre veil electrode. Journal of Solid State Electrochemistry, 12(10), 1317-1324. https://doi.org/10.1007/s10008-008-0536-0

Both nitrazepam and flunitrazepam have been determined by high-performance liquid chromatography dual electrode detection (LC-DED) in the reductive-reductive mode, using a carbon fibre veil electrode (CFVE) in conjugation with a glassy carbon electro... Read More about Determination of flunitrazepam and nitrazepam in beverage samples by liquid chromatography with dual electrode detection using a carbon fibre veil electrode.

Monitoring indoles and diamines: A screen-printed sensors and biosensors approach (2008)
Presentation / Conference
McGuire, N. D., Greenman, J., Bradshaw, D., & Hart, J. P. (2008, September). Monitoring indoles and diamines: A screen-printed sensors and biosensors approach. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool, UK

Real-time monitoring of glucose and lactate concentrations using screen-printed microband biosensors (2008)
Presentation / Conference
Pemberton, R., Xu, J., Pittson, R., Biddle, N., Drago, G., Griffiths, J., …Hart, J. P. (2008, September). Real-time monitoring of glucose and lactate concentrations using screen-printed microband biosensors. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool

Development of an anodic stripping voltammetric assay, using a disposable mercury-free screen-printed carbon electrode, for the determination of zinc in human sweat (2008)
Journal Article
Cowell, D. C., Crew, A. P., & Hart, J. P. (2008). Development of an anodic stripping voltammetric assay, using a disposable mercury-free screen-printed carbon electrode, for the determination of zinc in human sweat. Talanta, 75(5), 1221-1226. https://doi.org/10.1016/j.talanta.2008.01.043

This paper reports on the development of a novel electrochemical assay for Zn2+ in human sweat, which involves the use of disposable screen-printed carbon electrodes (SPCEs). Initially, SPCEs were used in conjunction with cyclic voltammetry to study... Read More about Development of an anodic stripping voltammetric assay, using a disposable mercury-free screen-printed carbon electrode, for the determination of zinc in human sweat.

Chapter 23 Screen-printed electrochemical (bio)sensors in biomedical, environmental and industrial applications (2007)
Journal Article
Hart, J. P., Crew, A., Crouch, E., Honeychurch, K. C., & Pemberton, R. (2007). Chapter 23 Screen-printed electrochemical (bio)sensors in biomedical, environmental and industrial applications. https://doi.org/10.1016/S0166-526X%2806%2949023-1

This chapter describes a number of approaches for the fabrication of prototype screen-printed electrochemical (bio) sensors together with selected applications in the areas of biomedical, environmental and industrial analyses. Novel methods of immobi... Read More about Chapter 23 Screen-printed electrochemical (bio)sensors in biomedical, environmental and industrial applications.

Voltammetric behaviour of p-nitrophenol and its trace determination in human urine by liquid chromatography with a dual reductive mode electrochemical detection system (2007)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2007). Voltammetric behaviour of p-nitrophenol and its trace determination in human urine by liquid chromatography with a dual reductive mode electrochemical detection system. Electroanalysis, 19(21), 2176-2184. https://doi.org/10.1002/elan.200703989

The determination of trace p-nitrophenol (PNP) concentrations in human urine has been successfully achieved by high performance liquid chromatography dual electrode detection (LC-DED) in the reduction-reduction mode. Initial cyclic voltammetric studi... Read More about Voltammetric behaviour of p-nitrophenol and its trace determination in human urine by liquid chromatography with a dual reductive mode electrochemical detection system.

Voltammetric behavior of p-nitrophenol and its trace determination in human urine by liquid chromatography with a dual reductive mode electrochemical detection system (2007)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2007). Voltammetric behavior of p-nitrophenol and its trace determination in human urine by liquid chromatography with a dual reductive mode electrochemical detection system. Electroanalysis, 19(21), 2176-2184. https://doi.org/10.1002/elan.200703989

The determination of trace p-nitrophenol (PNP) concentrations in human urine has been successfully achieved by high performance liquid chromatography dual electrode detection (LC-DED) in the reduction-reduction mode. Initial cyclic voltammetric studi... Read More about Voltammetric behavior of p-nitrophenol and its trace determination in human urine by liquid chromatography with a dual reductive mode electrochemical detection system.

Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat (2007)
Journal Article
Cowell, D. C., Crew, A. P., Alford, C., & Hart, J. P. (2007). Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat. Electrochimica Acta, 52(16), 5232-5237. https://doi.org/10.1016/j.electacta.2007.02.035

This paper reports on the steps involved in the development of a novel electrochemical immunoassay for secretory immunoglobulin A (sIgA) determinations in human sweat. This novel immunoassay involves an initial step whereby sweat sIgA is adsorbed ont... Read More about Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat.

Voltammetric behaviour of DNA bases at a screen-printed carbon electrode and its application to a simple and rapid voltammetric method for the determination of oxidative damage in double stranded DNA (2007)
Journal Article
O'Donovan, M. R., Honeychurch, K. C., & Hart, J. P. (2007). Voltammetric behaviour of DNA bases at a screen-printed carbon electrode and its application to a simple and rapid voltammetric method for the determination of oxidative damage in double stranded DNA. Biosensors and Bioelectronics, 22(9-10), 2057-2064. https://doi.org/10.1016/j.bios.2006.09.019

Screen-printed carbon electrodes (SPCEs) have been investigated as possible sensors to identify γ-irradiation induced oxidative damage in double stranded (ds) DNA. Studies were undertaken to explore the possibility of using both cyclic voltammetry an... Read More about Voltammetric behaviour of DNA bases at a screen-printed carbon electrode and its application to a simple and rapid voltammetric method for the determination of oxidative damage in double stranded DNA.

Selective and rapid biosensor integrated into a commercial hand-held instrument for the measurement of ammonium ion in sewage effluent (2006)
Journal Article
Hart, J. P., Serban, S., Jones, L. J., Biddle, N., Pittson, R., & Drago, G. A. (2006). Selective and rapid biosensor integrated into a commercial hand-held instrument for the measurement of ammonium ion in sewage effluent. Analytical Letters, 39(8), 1657-1667. https://doi.org/10.1080/00032710600713545

A disposable biosensor for ammonium ions in sewage effluent was developed and based on a screen-printed carbon electrode coated with the enzyme glutamate dehydrogenase, 2-oxoglutarate, and NADH. This rapid and selective amperometric biosensor is capa... Read More about Selective and rapid biosensor integrated into a commercial hand-held instrument for the measurement of ammonium ion in sewage effluent.

Studies towards the development of a screen-printed carbon electrochemical immunosensor array for mycotoxins: A sensor for aflatoxin B1 (2006)
Journal Article
Pemberton, R., Pittson, R., Biddle, N., Drago, G. A., & Hart, J. P. (2006). Studies towards the development of a screen-printed carbon electrochemical immunosensor array for mycotoxins: A sensor for aflatoxin B1. Analytical Letters, 39(8), 1573-1586. https://doi.org/10.1080/00032710600713289

Screen-printed carbon electrodes (SPCEs) bearing a surface-adsorbed antibody against aflatoxin B1 (AFB1) were used in a competitive immunoassay, based on competition of free analyte with a biotinylated aflatoxin B1 conjugate. Subsequent addition of s... Read More about Studies towards the development of a screen-printed carbon electrochemical immunosensor array for mycotoxins: A sensor for aflatoxin B1.

Voltammetric behavior of nitrazepam and its determination in serum using liquid chromatography with redox mode dual-electrode detection (2006)
Journal Article
Honeychurch, K. C., Smith, G. C., & Hart, J. P. (2006). Voltammetric behavior of nitrazepam and its determination in serum using liquid chromatography with redox mode dual-electrode detection. Analytical Chemistry, 78(2), 416-423. https://doi.org/10.1021/ac058035a

A method involving high-performance liquid chromatography with dual-electrode electrochemical detection in the redox mode (LC-DED) has been successfully developed for the determination of the benzodiazepine tranquilizer, nitrazepam, in serum. To eluc... Read More about Voltammetric behavior of nitrazepam and its determination in serum using liquid chromatography with redox mode dual-electrode detection.

Amperometric, screen-printed, glucose biosensor for analysis of human plasma samples using a biocomposite water-based carbon ink incorporating glucose oxidase (2005)
Journal Article
Crouch, E., Cowell, D. C., Hoskins, S., Pittson, R. W., & Hart, J. P. (2005). Amperometric, screen-printed, glucose biosensor for analysis of human plasma samples using a biocomposite water-based carbon ink incorporating glucose oxidase. Analytical Biochemistry, 347(1), 17-23. https://doi.org/10.1016/j.ab.2005.08.011

This paper describes the optimisation of a screen-printing water-based carbon ink containing cobalt phthalocyanine (CoPC) and glucose oxidase (GOD) for the fabrication of a glucose biosensor. To optimise the performance of the biosensor, the loadings... Read More about Amperometric, screen-printed, glucose biosensor for analysis of human plasma samples using a biocomposite water-based carbon ink incorporating glucose oxidase.

A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink (2005)
Journal Article
Pittson, R. W., Cowell, D. C., Crouch, E., Hoskins, S., & Hart, J. P. (2005). A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink. Biosensors and Bioelectronics, 21(5), 712-718. https://doi.org/10.1016/j.bios.2005.01.003

Screen-printed amperometric glucose biosensors have been fabricated using a water-based carbon ink. The enzyme glucose oxidase (GOD) and the electrocatalyst cobalt phthalocyanine were mixed with the carbon ink prior to the screen-printing process; th... Read More about A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink.

Amino acid determination using screen-printed electrochemical sensors (2005)
Journal Article
Merkoçi, A., Vasjari, M., Hart, J. P., & Alegret, S. (2005). Amino acid determination using screen-printed electrochemical sensors. Microchimica Acta, 150(3-4), 233-238. https://doi.org/10.1007/s00604-005-0361-4

The use of a screen-printed electrochemical sensor (SPES) strip, consisting of a two-electrode system (a carbon working electrode and Ag/AgCl reference electrode), for amino acid determination is reported. The behaviour of the SPES toward cysteine an... Read More about Amino acid determination using screen-printed electrochemical sensors.

Voltammetric determination of urinary 1-hydroxypyrene using molecularly imprinted polymer-modified screen-printed carbon electrodes (2005)
Journal Article
Kirsch, N., Honeychurch, K. C., Hart, J. P., & Whitcombe, M. J. (2005). Voltammetric determination of urinary 1-hydroxypyrene using molecularly imprinted polymer-modified screen-printed carbon electrodes. Electroanalysis, 17(7), 571-578. https://doi.org/10.1002/elan.200403131

A two step non-competitive affinity method for the trace determination of 1-hydroxypyrene (1-OHP) using a disposable molecularly imprinted polymer (MIP) modified screen-printed carbon electrode (MIP-SPCE) has been developed. The MIP was synthesized a... Read More about Voltammetric determination of urinary 1-hydroxypyrene using molecularly imprinted polymer-modified screen-printed carbon electrodes.

A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster (2004)
Journal Article
Hart, J. P., Crew, A. P., Hart, J. P., Wedge, R., Marty, J. L., & Fournier, D. (2004). A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster. Analytical Letters, 37(8), 1601-1610. https://doi.org/10.1081/AL-120037590

Screen-printed carbon electrodes (SPCEs) modified with cobalt phthalocyanine (CoPC) have been used as base transducers in the construction of amperometric pesticide biosensors. Six individual biosensors were fabricated by depositing wildtype (WT) ace... Read More about A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster.

Voltammetric determination of 8-oxo-guanine in the presence of guanine and adenine at a screen-printed carbon electrode, and its application for the determination of oxidative DNA damage (2004)
Presentation / Conference
Honeychurch, K. C., Hart, J. P., & O'Donovan, M. (2004, May). Voltammetric determination of 8-oxo-guanine in the presence of guanine and adenine at a screen-printed carbon electrode, and its application for the determination of oxidative DNA damage. Poster presented at Biosensors 2004, Granada, Spain

Some Recent Designs and Developments of Screen-Printed Carbon Electrochemical Sensors/Biosensors for Biomedical, Environmental, and Industrial Analyses (2004)
Journal Article
Hart, J. P., Crew, A. P., Crouch, E., Honeychurch, K. C., & Pemberton, R. (2004). Some Recent Designs and Developments of Screen-Printed Carbon Electrochemical Sensors/Biosensors for Biomedical, Environmental, and Industrial Analyses. Analytical Letters, 37(5), 789-830. https://doi.org/10.1081/AL-120030682

This review describes the design and fabrication of electrochemical sensors/biosensors based on screen-printing technology and their applications in pharmaceutical, biomedical, environmental, and industrial analyses. Specific emphasis is placed on na... Read More about Some Recent Designs and Developments of Screen-Printed Carbon Electrochemical Sensors/Biosensors for Biomedical, Environmental, and Industrial Analyses.

Voltammetric, chromatographic and mass spectral elucidation of the redox reactions of 1-hydroxypyrene occurring at a screen-printed carbon electrode (2004)
Journal Article
Honeychurch, K. C., Hart, J. P., & Kirsch, N. (2004). Voltammetric, chromatographic and mass spectral elucidation of the redox reactions of 1-hydroxypyrene occurring at a screen-printed carbon electrode. Electrochimica Acta, 49(7), 1141-1149. https://doi.org/10.1016/j.electacta.2003.10.025

The electrochemical oxidation of 1-hydroxypyrene (1-OHP) has been studied at a screen-printed carbon electrode (SPCE), by cyclic voltammetry and bulk electrolysis. Cyclic voltammograms exhibited two quasi-reversible pairs of peaks (designated 1a′/1c... Read More about Voltammetric, chromatographic and mass spectral elucidation of the redox reactions of 1-hydroxypyrene occurring at a screen-printed carbon electrode.

Screen-printed electrochemical sensors for monitoring metal pollutants (2003)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2003). Screen-printed electrochemical sensors for monitoring metal pollutants. Trends in Analytical Chemistry, 22(7), 456-469. https://doi.org/10.1016/S0165-9936%2803%2900703-9

This review describes current developments in the design and fabrication of disposable screen-printed sensors for the electrochemical determination of trace metals in biological, environmental and industrial samples. Specific emphasis is placed on se... Read More about Screen-printed electrochemical sensors for monitoring metal pollutants.

Sensors/biosensors, based on screen-printing technology for biomedical applications (2003)
Journal Article
Ryan, S. L., Hart, J. P., Abass, A., Honeychurch, K. C., Pemberton, R., & Wedge, R. (2003). Sensors/biosensors, based on screen-printing technology for biomedical applications. Indian Journal of Chemistry.section A.inorganic, Physical, Theoretical && Analytical, 42A(4), 709-718

This article describes three recent developments from our laboratory, in which screen-printed carbon electrodes have been modified in order to develop sensors/biosensors for analytes of biomedical importance. The analytical applications described are... Read More about Sensors/biosensors, based on screen-printing technology for biomedical applications.

Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode (2002)
Journal Article
Honeychurch, K. C., Hawkins, D. M., Hart, J. P., & Cowell, D. C. (2002). Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode. Talanta, 57(3), 565-574. https://doi.org/10.1016/S0039-9140%2802%2900060-7

Screen-printed carbon electrodes (SPCEs), without chemical modification, have been investigated as disposable sensors for the measurement of trace levels of Cu2+. Cyclic voltammetry was employed to elucidate the electrochemical behaviour of Cu2+ at t... Read More about Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode.

Voltammetric behavior and trace determination of cadmium at a calixarene modified screen-printed carbon electrode (2002)
Journal Article
Arrigan, D. W., Cowell, D. C., Hart, J. P., Honeychurch, K. C., Honeychurch, K. C., Hawkins, D. M., …Cowell, D. C. (2002). Voltammetric behavior and trace determination of cadmium at a calixarene modified screen-printed carbon electrode. Electroanalysis, 14(3), 177-185. https://doi.org/10.1002/1521-4109%28200202%2914%3A3%3C177%3A%3AAID-ELAN177%3E3.0.CO%3B2-V

Screen-printed carbon electrodes (SPCEs) have been evaluated for the anodic stripping voltammetric determination of cadmium using open circuit accumulation facilitated by two different calixarenes: a novel thiolated calixarene, calixarene (I), (5,11,... Read More about Voltammetric behavior and trace determination of cadmium at a calixarene modified screen-printed carbon electrode.

Voltammetric behavior and trace determination of cadmium at a calixarene modified screen-printed carbon electrode (2002)
Journal Article
Honeychurch, K. C., Hart, J. P., Cowell, D. C., & Arrigan, D. W. (2002). Voltammetric behavior and trace determination of cadmium at a calixarene modified screen-printed carbon electrode. Electroanalysis, 14(3), 177-185. https://doi.org/10.1002/1521-4109%28200202%2914%3A3%3C177%3A%3AAID-ELAN177%3E3.0.CO%3B2-V

Screen-printed carbon electrodes (SPCEs) have been evaluated for the anodic stripping voltammetric determination of cadmium using open circuit accumulation facilitated by two different calixarenes: a novel thiolated calixarene, calixarene (I), (5,11,... Read More about Voltammetric behavior and trace determination of cadmium at a calixarene modified screen-printed carbon electrode.

Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs (2001)
Journal Article
McCalley, D. V., Luxton, R. W., Bird, D. J., Hart, J. P., Kirsch, N., Kirsch, N., …McCalley, D. V. (2001). Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs. Analyst, 126(11), 1936-1941. https://doi.org/10.1039/b108008n

This paper describes the fabrication of a sensor for 1-hydroxypyrene (1-OHP) based on a screen-printed carbon electrode (SPCE) modified with a molecularly imprinted polymer (MIP); 1-OHP was chosen as a model metabolite of polyaromatic hydrocarbons (P... Read More about Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs.

Voltammetric studies of lead at calixarene modified screen-printed carbon electrodes and its trace determination in water by stripping voltammetry (2001)
Journal Article
Arrigan, D. W., Cowell, D. C., Hart, J. P., Honeychurch, K. C., Honeychurch, K. C., Hart, J. P., …Arrigan, D. W. (2001). Voltammetric studies of lead at calixarene modified screen-printed carbon electrodes and its trace determination in water by stripping voltammetry. Sensors and Actuators B: Chemical, 77(3), 642-652. https://doi.org/10.1016/S0925-4005%2801%2900778-X

Screen-printed carbon electrodes (SPCEs) have been modified with two different calixarenes; a novel thiolated calixarene (5,11,17,23,tetra-tert-butyl-25,26,27,28-tetrakis(2-mercaptoethoxy)- calix[4]arene), and the commercially available 25,26,27,28-t... Read More about Voltammetric studies of lead at calixarene modified screen-printed carbon electrodes and its trace determination in water by stripping voltammetry.

Voltammetric studies of lead at a 1-(2-pyridylazo)-2-naphthol modified screen-printed carbon electrode and its trace determination in water by stripping voltammetry (2001)
Journal Article
Honeychurch, K. C., Hart, J. P., & Cowell, D. C. (2001). Voltammetric studies of lead at a 1-(2-pyridylazo)-2-naphthol modified screen-printed carbon electrode and its trace determination in water by stripping voltammetry. Analytica Chimica Acta, 431(1), 89-99. https://doi.org/10.1016/S0003-2670%2800%2901294-0

A method for the trace determination of Pb2+, using a disposable 1-(2-pyridylazo)-2-naphthol (PAN) drop-coated modified screen-printed carbon electrode (PAN-SPCE), has been developed. Lead ions (Pb2+) are accumulated from ammonia buffer, on the PAN-S... Read More about Voltammetric studies of lead at a 1-(2-pyridylazo)-2-naphthol modified screen-printed carbon electrode and its trace determination in water by stripping voltammetry.

Voltammetric studies of lead at a 1-(2-pyridylazo)-2-naphthol modified screen-printed carbon electrode and its trace determination in water by stripping voltammetry (2001)
Journal Article
Honeychurch, K. C., Hart, J. P., & Cowell, D. C. (2001). Voltammetric studies of lead at a 1-(2-pyridylazo)-2-naphthol modified screen-printed carbon electrode and its trace determination in water by stripping voltammetry. Analytica Chimica Acta, 431(1), 89-99. https://doi.org/10.1016/S0003-2670%2800%2901294-0

A method for the trace determination of Pb2+, using a disposable 1-(2-pyridylazo)-2-naphthol (PAN) drop-coated modified screen-printed carbon electrode (PAN-SPCE), has been developed. Lead ions (Pb2+) are accumulated from ammonia buffer, on the PAN-S... Read More about Voltammetric studies of lead at a 1-(2-pyridylazo)-2-naphthol modified screen-printed carbon electrode and its trace determination in water by stripping voltammetry.

Voltammetric behavior and trace determination of lead at a mercury-free screen-printed carbon electrode (2000)
Journal Article
Honeychurch, K. C., Hart, J. P., & Cowell, D. C. (2000). Voltammetric behavior and trace determination of lead at a mercury-free screen-printed carbon electrode. Electroanalysis, 12(3), 171-177. https://doi.org/10.1002/%28SICI%291521-4109%28200002%2912%3A3%3C171%3A%3AAID-ELAN171%3E3.0.CO%3B2-Q

Abstract Screen-printed carbon electrodes (SPCEs), without chemical modification, have been investigated as disposable sensors for the measurement of trace levels of Pb. Cyclic voltammetry was employed to elucidate the electrochemical behavior of Pb... Read More about Voltammetric behavior and trace determination of lead at a mercury-free screen-printed carbon electrode.

A pilot-plant study of an automated quality control electronic nose for the monitoring of dry cured hams (1999)
Book Chapter
Abbass, A., de Lacy Costello, B., Cooper, L., Evans, P., Ewen, R., Hart, J. P., & Ratcliffe, N. M. (1999). A pilot-plant study of an automated quality control electronic nose for the monitoring of dry cured hams. In W. Hurst (Ed.), Electronic Noses & Sensor Array Based Systems - Design & Applications, Fifth International Symposium Proceedings (211-224). Pennsylvania, USA: CRC Press

A reagentless, screen-printed amperometric biosensor for the determination of glutamate in food and clinical applications
Book Chapter
Hughes, G., Pemberton, R., Fielden, P., & Hart, J. (2017). A reagentless, screen-printed amperometric biosensor for the determination of glutamate in food and clinical applications. In A. Rasooly, & B. Prickril (Eds.), Biosensors and Biodetection. Springer

A reagentless biosensor has been successfully developed to measure glutamate in food and clinical samples. The enzyme, glutamate dehydrogenase (GLDH) and the cofactor, nicotinamide adenine dinucleotide (NAD+) are fully integrated onto the surface of... Read More about A reagentless, screen-printed amperometric biosensor for the determination of glutamate in food and clinical applications.

Novel automated electrochemical system, based on a screen-printed amperometric biosensor array, for the simultaneous determination of organophosphate pesticides in environmental samples
Presentation / Conference
Crew, A. P., Pittson, K., Lonsdale, D., & Hart, J. P. Novel automated electrochemical system, based on a screen-printed amperometric biosensor array, for the simultaneous determination of organophosphate pesticides in environmental samples. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool

Studies towards the development of a prototype, disposable, screen-printed, electrochemical biosensor for phosphate ions in the acqueous environment
Presentation / Conference
Gilbert, L., Browning, S., Jenkins, T., & Hart, J. P. Studies towards the development of a prototype, disposable, screen-printed, electrochemical biosensor for phosphate ions in the acqueous environment. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool

Voltammetric and amperometric behaviour of reduced glutathione at a mircoband, cobalt phthalocyanine modified, screen-printed carbon electrode
Presentation / Conference
Honeychurch, K. C., & Hart, J. P. Voltammetric and amperometric behaviour of reduced glutathione at a mircoband, cobalt phthalocyanine modified, screen-printed carbon electrode. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool

Development of vapour phase, screen printed amperometric sensors for the measurement of indole and skatole: application to their determination in oral malodour
Presentation / Conference
McGuire, N. D., Greenman, J., Bradshaw, D., & Hart, J. P. Development of vapour phase, screen printed amperometric sensors for the measurement of indole and skatole: application to their determination in oral malodour. Paper presented at Great Western Electrochemistry Group (6th Annual Meeting), University of Bath

Fabrication, characterization and application of a glucose biosenser based on a screen-printed carbon electrode incorporating insoluble meldola's blue and glucose dehydrogenase
Presentation / Conference
Piano, M., Pittson, R., Drago, G., Serban, S., & Hart, J. P. Fabrication, characterization and application of a glucose biosenser based on a screen-printed carbon electrode incorporating insoluble meldola's blue and glucose dehydrogenase. Paper presented at Electrochemistry Group, Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Livepool

The characterization of screen-printed microband electrodes for NADH detection released from human cells
Presentation / Conference
Rawson, F., Purcell, W., Xu, J., Cowell, D., Fieldon, P., Biddle, N., & Hart, J. P. The characterization of screen-printed microband electrodes for NADH detection released from human cells. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool