Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode
Nicholas, Phil; Pittson, Robin; Hart, John P.
John Hart John.Hart@uwe.ac.uk
© 2017 Elsevier Ltd This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies. Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 mM with a precision of 3.03% (n = 5). Following fortification (477 mM fructose) the mean recovery was found to be 97.12% with a coefficient of variation of 6.42% (n = 5); consequently, the method holds promise for the analysis of commercial fruit juices.
Nicholas, P., Pittson, R., & Hart, J. P. (2018). Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode. Food Chemistry, 241, 122-126. https://doi.org/10.1016/j.foodchem.2017.08.077
|Journal Article Type||Article|
|Acceptance Date||Aug 22, 2017|
|Online Publication Date||Aug 23, 2017|
|Publication Date||Feb 15, 2018|
|Peer Reviewed||Peer Reviewed|
|Keywords||fructose, fructose dehydrogenase, chronoamperometric, screen-printed, graphite, nanoparticles|