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Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode

Nicholas, Phil; Pittson, Robin; Hart, John P.

Authors

Phil Nicholas

Robin Pittson



Abstract

© 2017 Elsevier Ltd This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies. Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 mM with a precision of 3.03% (n = 5). Following fortification (477 mM fructose) the mean recovery was found to be 97.12% with a coefficient of variation of 6.42% (n = 5); consequently, the method holds promise for the analysis of commercial fruit juices.

Citation

Nicholas, P., Pittson, R., & Hart, J. P. (2018). Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode. Food Chemistry, 241, 122-126. https://doi.org/10.1016/j.foodchem.2017.08.077

Journal Article Type Article
Acceptance Date Aug 22, 2017
Online Publication Date Aug 23, 2017
Publication Date Feb 15, 2018
Journal Food Chemistry
Print ISSN 0308-8146
Electronic ISSN 1873-7072
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 241
Pages 122-126
DOI https://doi.org/10.1016/j.foodchem.2017.08.077
Keywords fructose, fructose dehydrogenase, chronoamperometric, screen-printed, graphite, nanoparticles
Public URL https://uwe-repository.worktribe.com/output/871459
Publisher URL http://dx.doi.org/10.1016/j.foodchem.2017.08.077