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A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors

Westmacott, Kelly L; Crew, Adrian P; Doran, Olena; Hart, John P

Authors

Kelly L Westmacott

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Dr Adrian Crew Adrian.Crew@uwe.ac.uk
Senior Lecturer in Environmental Microbiology



Abstract

© 2017 Elsevier B.V. This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B1 (thiamine), B2 (riboflavin) and B6 (pyridoxine) in phosphate buffer were established using cyclic voltammetry in conjunction with screen printed carbon electrodes (SPCEs). The optimum current response for all three vitamins was achieved in 0.1 M phosphate buffer pH 11 using an initial potential of −1.0 V. Using square wave voltammetry, the linear ranges for thiamine, riboflavin, and pyridoxine were found to be: 15–110 µg/ml, 0.1–20 µg/ml, and 2–80 µg/ml respectively. The application of the method to a commercial food product yielded a recovery of 95.78% for riboflavin, with a coefficient of variation (CV) of 3.38% (n = 5). The method was also applied to a multi-vitamin supplement for the simultaneous determination of thiamine, riboflavin and pyridoxine. In both cases only simple dilution with buffer followed by centrifugation was required prior to analysis. The resulting square wave voltammetric signals were completely resolved with Ep values of −0.7 V, +0.2 V, and +0.6 V respectively. The recoveries determined for the vitamin B complex in a commercial supplement product were found to be 110%, 114%, and 112% respectively (CV = 7.14%, 6.28%. 5.66% respectively, n = 5).

Citation

Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2018). A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors. Talanta, 181, 13-18. https://doi.org/10.1016/j.talanta.2017.12.074

Journal Article Type Article
Acceptance Date Dec 22, 2017
Online Publication Date Dec 29, 2017
Publication Date May 1, 2018
Journal Talanta
Print ISSN 0039-9140
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 181
Pages 13-18
DOI https://doi.org/10.1016/j.talanta.2017.12.074
Keywords cyclic, square-wave, voltammetry, thiamine, riboflavin, pyridoxine
Public URL https://uwe-repository.worktribe.com/output/876463
Publisher URL http://dx.doi.org/10.1016/j.talanta.2017.12.074
Related Public URLs https://www.sciencedirect.com/science/article/pii/S0039914017312766