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An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements

Smart, Amy; Westmacott, Kelly L.; Crew, Adrian; Doran, Olena; Hart, John P.

Authors

Amy Smart

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Dr Adrian Crew Adrian.Crew@uwe.ac.uk
Senior Lecturer in Environmental Microbiology



Abstract

An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its electrochemically active thiolate anion species. It was shown that an electrocatalytic oxidation reaction occurred with the screen-printed carbon electrode containing the mediator cobalt phthalocyanine (CoPC-SPCE). This had the advantage of producing an analytical response current at an operating potential of 0 V vs. Ag/AgCl compared to +0.34 V obtained with plain SPCEs. This resulted in improved selectivity and limit of detection. Detailed studies on the underlying mechanism occurring with the sensor are reported in this paper. A linear response was obtained between 0.1 and 20 µg mL −1 , which was suitable for the quantification of the vitamin in two commercial products containing vitamin B1. The mean recovery for a multivitamin tablet with a declared content of 5 mg was 101% (coefficient of variation (CV) of 9.6%). A multivitamin drink, which had a much lower concentration of vitamin B1 (0.22 mg/100 mL), gave a mean recovery of 93.3% (CV 7.2%). These results indicate that our sensor holds promise for quality control of food supplements and other food types.

Citation

Smart, A., Westmacott, K. L., Crew, A., Doran, O., & Hart, J. P. (2019). An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements. Biosensors, 9(3), https://doi.org/10.3390/bios9030098

Journal Article Type Article
Acceptance Date Aug 2, 2019
Online Publication Date Aug 6, 2019
Publication Date Sep 1, 2019
Deposit Date Aug 20, 2019
Publicly Available Date Aug 21, 2019
Journal Biosensors
Electronic ISSN 2079-6374
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 9
Issue 3
Article Number 98
DOI https://doi.org/10.3390/bios9030098
Keywords cobalt phthalocyanine; screen-printed carbon electrode; cyclic voltammetry; amperometry; thiamine; food supplements
Public URL https://uwe-repository.worktribe.com/output/2314795

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