Skip to main content

Research Repository

Advanced Search

Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method

Westmacott, K. L.; Crew, A. P.; Doran, O.; Hart, J. P.

Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method Thumbnail


Authors

Profile Image

Dr Adrian Crew Adrian.Crew@uwe.ac.uk
Senior Lecturer in Environmental Microbiology



Abstract

© 2019 Elsevier B.V. This is the first report on the fabrication, characterisation and application of an electrochemical (bio)sensor system for the simultaneous measurement of skatole and androstenone. A biosensor for androstenone was fabricated using a Meldola's Blue modified SPCE (MB-SPCE) by depositing NADH and the enzyme 3α-hydroxysteroid dehydrogenase onto the MB-SPCE surface; samples of adipose tissue were analysed using the biosensors in conjunction with chronoamperometry. Cyclic voltammetry was used to investigate the electrochemical behaviour of skatole at a screen-printed carbon electrode (SPCE vs. Ag/AgCl). An oxidation peak was observed around +0.55 V (vs. Ag/AgCl) and differential pulse voltammetry was applied for quantification of skatole in adipose tissue (in-situ). Quantitative analysis was achieved using calibration plots obtained from fortified meat samples. The concentrations obtained by the electrochemical and gas chromatographic (GC) methods demonstrated a good positive correlation. The (bio)sensor system completed both measurements within 60 s, as compared to several hours for GC, and at a considerably reduced cost and complexity. Consequently, the novel (bio)sensor system should have applications for analysis of carcasses on the abattoir processing line.

Citation

Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2020). Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method. Biosensors and Bioelectronics, 150, https://doi.org/10.1016/j.bios.2019.111837

Journal Article Type Article
Acceptance Date Oct 31, 2019
Online Publication Date Nov 5, 2019
Publication Date Feb 15, 2020
Deposit Date Nov 8, 2019
Publicly Available Date Nov 6, 2020
Journal Biosensors and Bioelectronics
Print ISSN 0956-5663
Electronic ISSN 1873-4235
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 150
Article Number 111837
DOI https://doi.org/10.1016/j.bios.2019.111837
Keywords Biotechnology; Biophysics; Electrochemistry; Biomedical Engineering; General Medicine; Boar taint; Screen-printing; Biosensor; Sensor; Differential pulse voltammetry; Chronoamperometry; GC-FID; GC-NPD
Public URL https://uwe-repository.worktribe.com/output/4439276
Publisher URL https://www.sciencedirect.com/journal/biosensors-and-bioelectronics

Files







You might also like



Downloadable Citations