Kelly Westmacott Kelly.Westmacott@uwe.ac.uk
Research Associate - Smart Dressing
Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method
Westmacott, K. L.; Crew, A. P.; Doran, O.; Hart, J. P.
Authors
Dr Adrian Crew Adrian.Crew@uwe.ac.uk
Senior Lecturer in Environmental Microbiology
Olena Doran Olena.Doran@uwe.ac.uk
College Dean of Research and Enterprise
John Hart John.Hart@uwe.ac.uk
Professor
Abstract
© 2019 Elsevier B.V. This is the first report on the fabrication, characterisation and application of an electrochemical (bio)sensor system for the simultaneous measurement of skatole and androstenone. A biosensor for androstenone was fabricated using a Meldola's Blue modified SPCE (MB-SPCE) by depositing NADH and the enzyme 3α-hydroxysteroid dehydrogenase onto the MB-SPCE surface; samples of adipose tissue were analysed using the biosensors in conjunction with chronoamperometry. Cyclic voltammetry was used to investigate the electrochemical behaviour of skatole at a screen-printed carbon electrode (SPCE vs. Ag/AgCl). An oxidation peak was observed around +0.55 V (vs. Ag/AgCl) and differential pulse voltammetry was applied for quantification of skatole in adipose tissue (in-situ). Quantitative analysis was achieved using calibration plots obtained from fortified meat samples. The concentrations obtained by the electrochemical and gas chromatographic (GC) methods demonstrated a good positive correlation. The (bio)sensor system completed both measurements within 60 s, as compared to several hours for GC, and at a considerably reduced cost and complexity. Consequently, the novel (bio)sensor system should have applications for analysis of carcasses on the abattoir processing line.
Citation
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2020). Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method. Biosensors and Bioelectronics, 150, Article 111837. https://doi.org/10.1016/j.bios.2019.111837
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 31, 2019 |
Online Publication Date | Nov 5, 2019 |
Publication Date | Feb 15, 2020 |
Deposit Date | Nov 8, 2019 |
Publicly Available Date | Nov 6, 2020 |
Journal | Biosensors and Bioelectronics |
Print ISSN | 0956-5663 |
Electronic ISSN | 1873-4235 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 150 |
Article Number | 111837 |
DOI | https://doi.org/10.1016/j.bios.2019.111837 |
Keywords | Biotechnology; Biophysics; Electrochemistry; Biomedical Engineering; General Medicine; Boar taint; Screen-printing; Biosensor; Sensor; Differential pulse voltammetry; Chronoamperometry; GC-FID; GC-NPD |
Public URL | https://uwe-repository.worktribe.com/output/4439276 |
Publisher URL | https://www.sciencedirect.com/journal/biosensors-and-bioelectronics |
Files
Novel, Rapid, Low-cost Screen-printed (bio)sensors For The Direct Analysis Of Boar Taint Compounds Androstenone And Skatole In Porcine Adipose Tissue: Comparison With A High-resolution Gas Chromatographic Method
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Licence
http://creativecommons.org/licenses/by-nc-nd/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/
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