Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method

Westmacott, K. L.; Crew, A. P.; Doran, O.; Hart, J. P.

Abstract

© 2019 Elsevier B.V. This is the first report on the fabrication, characterisation and application of an electrochemical (bio)sensor system for the simultaneous measurement of skatole and androstenone. A biosensor for androstenone was fabricated using a Meldola's Blue modified SPCE (MB-SPCE) by depositing NADH and the enzyme 3α-hydroxysteroid dehydrogenase onto the MB-SPCE surface; samples of adipose tissue were analysed using the biosensors in conjunction with chronoamperometry. Cyclic voltammetry was used to investigate the electrochemical behaviour of skatole at a screen-printed carbon electrode (SPCE vs. Ag/AgCl). An oxidation peak was observed around +0.55 V (vs. Ag/AgCl) and differential pulse voltammetry was applied for quantification of skatole in adipose tissue (in-situ). Quantitative analysis was achieved using calibration plots obtained from fortified meat samples. The concentrations obtained by the electrochemical and gas chromatographic (GC) methods demonstrated a good positive correlation. The (bio)sensor system completed both measurements within 60 s, as compared to several hours for GC, and at a considerably reduced cost and complexity. Consequently, the novel (bio)sensor system should have applications for analysis of carcasses on the abattoir processing line.

• Journal Article Type: Article
• Acceptance Date: Oct 31, 2019
• Online Publication Date: Nov 5, 2019
• Publication Date: Feb 15, 2020
• Deposit Date: Nov 8, 2019
• Publicly Available Date: Nov 6, 2020
• Journal: Biosensors and Bioelectronics
• Print ISSN: 0956-5663
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 150
• Article Number: 111837
• DOI: https://doi.org/10.1016/j.bios.2019.111837
• Keywords: Biotechnology; Biophysics; Electrochemistry; Biomedical Engineering; General Medicine; Boar taint; Screen-printing; Biosensor; Sensor; Differential pulse voltammetry; Chronoamperometry; GC-FID; GC-NPD
• Public URL: https://uwe-repository.worktribe.com/output/4439276
• Publisher URL: https://www.sciencedirect.com/journal/biosensors-and-bioelectronics