Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode

Nicholas, Phil; Pittson, Robin; Hart, John P.

Abstract

© 2017 Elsevier Ltd This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies. Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 mM with a precision of 3.03% (n = 5). Following fortification (477 mM fructose) the mean recovery was found to be 97.12% with a coefficient of variation of 6.42% (n = 5); consequently, the method holds promise for the analysis of commercial fruit juices.

• Journal Article Type: Article
• Acceptance Date: Aug 22, 2017
• Online Publication Date: Aug 23, 2017
• Publication Date: Feb 15, 2018
• Deposit Date: Sep 20, 2017
• Publicly Available Date: Aug 23, 2018
• Journal: Food Chemistry
• Print ISSN: 0308-8146
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 241
• Pages: 122-126
• DOI: https://doi.org/10.1016/j.foodchem.2017.08.077
• Keywords: fructose, fructose dehydrogenase, chronoamperometric, screen-printed, graphite, nanoparticles
• Public URL: https://uwe-repository.worktribe.com/output/871459
• Publisher URL: http://dx.doi.org/10.1016/j.foodchem.2017.08.077
• Contract Date: Sep 20, 2017