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Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode

Nicholas, Phil; Pittson, Robin; Hart, John P.

Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode Thumbnail


Authors

Phil Nicholas

Robin Pittson



Abstract

© 2017 Elsevier Ltd This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies. Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 mM with a precision of 3.03% (n = 5). Following fortification (477 mM fructose) the mean recovery was found to be 97.12% with a coefficient of variation of 6.42% (n = 5); consequently, the method holds promise for the analysis of commercial fruit juices.

Journal Article Type Article
Acceptance Date Aug 22, 2017
Online Publication Date Aug 23, 2017
Publication Date Feb 15, 2018
Deposit Date Sep 20, 2017
Publicly Available Date Aug 23, 2018
Journal Food Chemistry
Print ISSN 0308-8146
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 241
Pages 122-126
DOI https://doi.org/10.1016/j.foodchem.2017.08.077
Keywords fructose, fructose dehydrogenase, chronoamperometric, screen-printed, graphite, nanoparticles
Public URL https://uwe-repository.worktribe.com/output/871459
Publisher URL http://dx.doi.org/10.1016/j.foodchem.2017.08.077
Contract Date Sep 20, 2017