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Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour

Hughes, Gareth; Pemberton, Roy M; Nicholas, Phil; Hart, John P

Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour Thumbnail


Authors

Gareth Hughes

Phil Nicholas



Abstract

This paper describes a simple, convenient approach to the fabrication of microband electrodes and microband biosensors based on screen printing technology. These devices were printed in a three-electrode configuration on one strip; a silver/silver chloride electrode and carbon counter electrode served as reference and counter electrodes respectively. The working electrodes were fabricated by screen-printing a water-based carbon ink containing cobalt phthalocyanine for hydrogen peroxide detection. These were converted into a glucose microband biosensor by the addition of glucose oxidase into the carbon ink. In this paper, we discuss the fabrication and application of glucose microband electrodes for the determination of glucose in cell media. The dimensions (100 – 400 microns) of the microband electrodes result in radial diffusion, which results in steady state responses in the absence of stirring. The microband biosensors were investigated in cell media containing different concentrations of glucose using chronoamperometry. The device shows linearity for glucose determination in the range 0.5 mM to 2.5 mM in cell media. The screen-printed microband biosensor design holds promise as a generic platform for future applications in cell toxicity studies.

Citation

Hughes, G., Pemberton, R. . M., Nicholas, P., & Hart, J. P. (2018). Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour. Electroanalysis, 30(8), 1608-1612. https://doi.org/10.1002/elan.+201+800+104

Journal Article Type Article
Acceptance Date Mar 24, 2018
Online Publication Date Apr 25, 2018
Publication Date Aug 31, 2018
Deposit Date May 15, 2018
Publicly Available Date Mar 29, 2024
Journal Electroanalysis
Print ISSN 1040-0397
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 30
Issue 8
Pages 1608-1612
DOI https://doi.org/10.1002/elan.+201+800+104
Keywords water-based ink, glucose oxidase, microband biosensor, cyclic voltammetry, chronoamperometry, hydrogen peroxide
Public URL https://uwe-repository.worktribe.com/output/862069
Publisher URL https://doi.org/10.1002/elan.201800104
Additional Information Additional Information : This is the peer reviewed version of the following article: [Hughes, G., Pemberton, R. M., Nicholas, P. and Hart, J. P. (2018) Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour. Electroanalysis. ISSN 1040-0397], which has been published in final form at https://doi.org/10.1002/elan.201800104. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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