Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications

Pemberton, Roy M.; Jackson, Simon K.; Kanyong, Prosper; Pemberton, Roy; Hughes, Gareth; Jackson, Simon; Hart, John P.

Abstract

© 2016, Copyright © Taylor & Francis Group, LLC. ABSTRACT: This paper reports the development and application of a biosensor for the amperometric determination of galactose in the presence of human hepatocellular carcinoma cells with and without a hepatotoxic agent. The biosensor was fabricated by drop-coating 1.5% cellulose acetate on a 3×3mm screen-printed carbon electrode followed by depositing 2 U of galactose oxidase. The electrodes dimensions were reduced to 3×0.5mm before measurements. Hepatocellular carcinoma cells were utilized for in vitro toxicity testing by evaluating the effect of paracetamol on galactose uptake. The amperometric responses to galactose indicated that the inhibition of uptake was directly proportional to the concentration of paracetamol following 24h of exposure to the hepatocellular carcinoma cells. These results demonstrate that the fabricated biosensor may be used for the real-time monitoring of cell metabolism and toxicity.

• Journal Article Type: Article
• Acceptance Date: Jul 2, 2015
• Publication Date: Jan 22, 2016
• Deposit Date: Jan 25, 2016
• Publicly Available Date: Aug 12, 2016
• Journal: Analytical Letters
• Print ISSN: 0003-2719
• Electronic ISSN: 1532-236X
• Publisher: Taylor & Francis
• Peer Reviewed: Peer Reviewed
• Volume: 49
• Issue: 2
• Pages: 236-244
• DOI: https://doi.org/10.1080/00032719.2015.1070166
• Keywords: amperometry, biosensor, screen-printed electrode
• Public URL: https://uwe-repository.worktribe.com/output/915754
• Publisher URL: http://dx.doi.org/10.1080/00032719.2015.1070166
• Additional Information: Additional Information : This is an Accepted Manuscript of an article published by Taylor & Francis in Analytical Letters in January 2016, available online: http://wwww.tandfonline.com/10.1080/00032719.2015.1070166
• Contract Date: May 7, 2016