Evaluation of a novel disposable amperometric glycerol biosensor based on a meldolas blue-modified screen-printed carbon electrode for juice beverage analysis

Ekonomou, Dr Sotirios I.; Crew, Dr Adrian; Olena, Prof Doran; Hart, Prof John P.

Abstract

This study details the design and development of an electrochemical biosensor for measuring glycerol, an important compound in food and beverages, due to its role as a naturally occurring triose sugar and food additive (E 422). Monitoring glycerol concentration is essential for ensuring food quality and safety. The biosensor operates by enzymatically oxidizing glycerol using glycerol dehydrogenase (GLDH) in the presence of oxidized Nicotinamide adenine dinucleotide (NAD+), resulting in the production of its reduced form (NADH) and dihydroxyacetone. The NADH then undergoes electrocatalytic oxidation at a Meldolas Blue-modified Screen-printed Carbon Electrode (MB-SPCE), with the resulting current serving as the analytical response. This response is directly proportional to the glycerol concentration. Standard addition calibration studies using chronoamperometry were performed with glycerol concentrations between 1.0 to 3.0 mM, utilizing only 100 uL of diluted (0.1 M phosphate buffer) grape juice (GJ) directly on the biosensor surface. Calibration plots were constructed by taking current measurements at 100 s after application of the applied potential; this demonstrated that the glycerol biosensor produced a linear response across the concentration range studied. The amperometric biosensor was successfully applied to the measurement of glycerol in commercially available GJ, representing non-alcoholic beverages. These analyses have the ability to be conducted outside the laboratory using commercially available, portable potentiostats. Overall, this approach shows promise to form a platform for the development of novel rapid technology for point-of-test evaluation of glycerol in the production and quality control of non-alcoholic beverages.

• Presentation Conference Type: Conference Paper (published)
• Conference Name: 5th International Congress, Food Technology, Quality and Safety
• Start Date: Oct 16, 2024
• End Date: Oct 18, 2024
• Acceptance Date: Sep 30, 2024
• Online Publication Date: Oct 24, 2024
• Publication Date: Nov 24, 2024
• Deposit Date: Nov 11, 2024
• Publicly Available Date: Nov 25, 2024
• Peer Reviewed: Peer Reviewed
• Pages: 27-33
• Book Title: Proceedings in the 5th International Congress in Food Technology, Quality and Safety
• ISBN: 9788679940643
• Keywords: Food safety and quality, amperometric glycerol biosensor, chronoamperometry, screen-printed carbon electrode, Meldolas Blue, grape juice
• Public URL: https://uwe-repository.worktribe.com/output/13414308
• Publisher URL: https://foodtech.uns.ac.rs/wp-content/uploads/2018/07/e-PROCEEDINGS-FoodTech2024-1.pdf