Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids

Smart, A.; Crew, A.; Doran, O.; Hart, J.P.

Abstract

This paper describes, for the first time, the development of a novel trilinolein biosensor based on the immobilisation of lipase and lipoxygenase (LOX) onto a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) in conjunction with amperometry in stirred solution. The combination of enzymes, integrated into the novel biosensor, provide a selective response for polyunsaturated fatty acid (PUFA) triglycerides, in the presence of both monounsaturated (MUFA) and saturated fatty acid (SFA) triglycerides. The linear range of the trilinolein biosensor is 0.2–10 µM, and the detection limit is 45.5 nM. The biosensor was successfully applied to the measurement of trilinolein present in a pharmaceutical food supplement. The percentage recovery was 86% and the coefficient of variation of the biosensor response was 5.05%. This novel LOX-lipase biosensor is simple to manufacture for trilinolein analysis and has advantages of low cost, speed of analysis and ease of operation, thus making it commercially attractive for a range of applications including food and clinical samples.

• Journal Article Type: Article
• Acceptance Date: Jun 22, 2023
• Online Publication Date: Jun 23, 2023
• Publication Date: Sep 1, 2023
• Deposit Date: Jun 26, 2023
• Publicly Available Date: Jun 28, 2023
• Journal: Journal of Food Composition and Analysis
• Print ISSN: 0889-1575
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 122
• Article Number: 105488
• DOI: https://doi.org/10.1016/j.jfca.2023.105488
• Keywords: Food Science, Biosensing, Electrochemical, Fatty Acids, Biosensor, Amperometry, Screen printed carbon electrode, Fatty acid, Triglyceride, Trilinolein
• Public URL: https://uwe-repository.worktribe.com/output/10889325
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0889157523003629
• Additional Information: This article is maintained by: Elsevier; Article Title: Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids; Journal Title: Journal of Food Composition and Analysis; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jfca.2023.105488; Content Type: article; Copyright: © 2023 The Author(s). Published by Elsevier Inc.