Skip to main content

Research Repository

Advanced Search

Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids

Smart, A.; Crew, A.; Doran, O.; Hart, J.P.

Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids Thumbnail


Authors

A. Smart

Profile Image

Dr Adrian Crew Adrian.Crew@uwe.ac.uk
Senior Lecturer in Environmental Microbiology

Olena Doran Olena.Doran@uwe.ac.uk
College Dean of Research and Enterprise



Abstract

This paper describes, for the first time, the development of a novel trilinolein biosensor based on the immobilisation of lipase and lipoxygenase (LOX) onto a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) in conjunction with amperometry in stirred solution. The combination of enzymes, integrated into the novel biosensor, provide a selective response for polyunsaturated fatty acid (PUFA) triglycerides, in the presence of both monounsaturated (MUFA) and saturated fatty acid (SFA) triglycerides. The linear range of the trilinolein biosensor is 0.2–10 µM, and the detection limit is 45.5 nM. The biosensor was successfully applied to the measurement of trilinolein present in a pharmaceutical food supplement. The percentage recovery was 86% and the coefficient of variation of the biosensor response was 5.05%. This novel LOX-lipase biosensor is simple to manufacture for trilinolein analysis and has advantages of low cost, speed of analysis and ease of operation, thus making it commercially attractive for a range of applications including food and clinical samples.

Citation

Smart, A., Crew, A., Doran, O., & Hart, J. (2023). Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids. Journal of Food Composition and Analysis, 122, Article 105488. https://doi.org/10.1016/j.jfca.2023.105488

Journal Article Type Article
Acceptance Date Jun 22, 2023
Online Publication Date Jun 23, 2023
Publication Date Sep 1, 2023
Deposit Date Jun 26, 2023
Publicly Available Date Jun 28, 2023
Journal Journal of Food Composition and Analysis
Print ISSN 0889-1575
Electronic ISSN 1096-0481
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 122
Article Number 105488
DOI https://doi.org/10.1016/j.jfca.2023.105488
Keywords Food Science, Biosensing, Electrochemical, Fatty Acids, Biosensor, Amperometry, Screen printed carbon electrode, Fatty acid, Triglyceride, Trilinolein
Public URL https://uwe-repository.worktribe.com/output/10889325
Publisher URL https://www.sciencedirect.com/science/article/pii/S0889157523003629
Additional Information This article is maintained by: Elsevier; Article Title: Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids; Journal Title: Journal of Food Composition and Analysis; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jfca.2023.105488; Content Type: article; Copyright: © 2023 The Author(s). Published by Elsevier Inc.

Files









Related Outputs



You might also like



Downloadable Citations