Skip to main content

Research Repository

Advanced Search

All Outputs (61)

Genetic test for food quality (2009)
Presentation / Conference
Doran, O. (2009, January). Genetic test for food quality. Paper presented at Abstracts of Lectures and Posters of International Conference Repid Methods Europe 2009, Noordwijkerhout, The Netherlands

Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes (2008)
Journal Article
Panella-Riera, N., Moe, M., Grindflek, E., Oliver, M. A., Wood, J. D., & Doran, O. (2008). Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes. Livestock Science, 118(3), 223-230. https://doi.org/10.1016/j.livsci.2008.01.021

An excessive accumulation of androstenone (5α-androst-16-en-3-one) in pig adipose tissue is one of the two major contributors to the phenomenon of boar taint. High levels of adipose tissue androstenone have been related to a low rate of hepatic andro... Read More about Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes.

Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes (2008)
Journal Article
Nicolau-Solano, S. I., & Doran, O. (2008). Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes. Livestock Science, 114(2-3), 202-210. https://doi.org/10.1016/j.livsci.2007.05.001

Androstenone (5α-androst-16-en-3-one) is a steroid pheromone, excessive accumulation of which in pig adipose tissue contributes to the phenomenon of "boar taint". The incidence of boar taint increases with age and weight of entire male pigs. One of t... Read More about Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes.

Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes (2008)
Journal Article
Wood, J. D., Chen, G., Cue, R. A., Lundstrom, K., & Doran, O. (2008). Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes. Drug Metabolism and Disposition, 36(1), 56-60. https://doi.org/10.1124/dmd.107.017285

CYP2A6 is one of the enzymes involved in the hepatic metabolism of a naturally produced compound, skatole, in the pig. Low CYP2A6 activity has been linked to excessive accumulation of skatole in pig adipose tissue and development of the phenomenon "b... Read More about Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes.

Expression of 3β-hydroxysteroid dehydrogenase, cytochrome P450-c17, and sulfotransferase 2B1 proteins in liver and testis of pigs of two breeds: Relationship with adipose tissue androstenone concentration (2007)
Journal Article
Moe, M., Moe, E., Grindflek, E., & Doran (nee Udovikova), O. (2007). Expression of 3β-hydroxysteroid dehydrogenase, cytochrome P450-c17, and sulfotransferase 2B1 proteins in liver and testis of pigs of two breeds: Relationship with adipose tissue androstenone concentration. Journal of Animal Science, 85(11), 2924-2931. https://doi.org/10.2527/jas.2007-0283

An excessive accumulation of androstenone in pig adipose tissue is a major contributor to the phenomenon of boar taint. Androstenone deposition is dependent on the rate of androstenone biosynthesis in testis and androstenone degradation in liver. The... Read More about Expression of 3β-hydroxysteroid dehydrogenase, cytochrome P450-c17, and sulfotransferase 2B1 proteins in liver and testis of pigs of two breeds: Relationship with adipose tissue androstenone concentration.

Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3β-hydroxysteroid dehydrogenase in entire commercial pigs (2007)
Journal Article
Nicolau-Solano, S. I., Wittington, F. M., Wood, J. D., & Doran (nee Udovikova), O. (2007). Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3β-hydroxysteroid dehydrogenase in entire commercial pigs. animal, 1(7), 1053-1059. https://doi.org/10.1017/S1751731107000274

Boar taint is a major meat-quality defect in pigs and is due to excessive accumulation of skatole and androstenone in adipose tissue. The present work investigated the relationship between carcass weight, levels of skatole and androstenone in adipose... Read More about Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3β-hydroxysteroid dehydrogenase in entire commercial pigs.

Breed-associated variations in the sequence of the pig 3β- hydroxysteroid dehydrogenase gene (2007)
Journal Article
Cue, R. A., Nicolau-Solano, S., McGivan, J. D., Wood, J. D., & Doran, O. (2007). Breed-associated variations in the sequence of the pig 3β- hydroxysteroid dehydrogenase gene. Journal of Animal Science, 85(3), 571-576. https://doi.org/10.2527/jas.2006-366

The entire sequence of the pig 3β-hydroxysteroid dehydrogenase (3β-HSD) gene has recently become known. This gene is deemed to be important in androstenone metabolism in pig liver, and its defective expression has been shown to be related to androste... Read More about Breed-associated variations in the sequence of the pig 3β- hydroxysteroid dehydrogenase gene.

Relationship between the expression of hepatic but not testicular 3β-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue (2006)
Journal Article
Wood, J. D., Whittington, F. M., McGivan, J. D., Nicolau-Solano, S. I., Nieuwhof, G. J., & Doran (nee Udovikova), O. (2006). Relationship between the expression of hepatic but not testicular 3β-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue. Journal of Animal Science, 84(10), 2809-2817. https://doi.org/10.2527/jas.2005-595

This study investigated the relationship between expression of hepatic and testicular 3/3-hydroxysteroid dehydrogenase (3/3-HSD) and accumulation of androstenone in adipose tissue because of its relation to boar taint. The experiments were performed... Read More about Relationship between the expression of hepatic but not testicular 3β-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue.

A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: Relationship with intramuscular lipid formation (2006)
Journal Article
Doran (nee Udovikova), O., Moule, S. K., Teye, G. A., Whittington, F. M., Hallett, K. G., & Wood, J. D. (2006). A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: Relationship with intramuscular lipid formation. British Journal of Nutrition, 95(3), 609-617. https://doi.org/10.1079/BJN20051526

A reduced protein diet (RPD) is known to increase the level of intramuscular lipid in pig meat with a smaller effect on the amount of subcutaneous adipose tissue. This might be due to tissue-specific activation of the expression of lipogenic enzymes... Read More about A reduced protein diet induces stearoyl-CoA desaturase protein expression in pig muscle but not in subcutaneous adipose tissue: Relationship with intramuscular lipid formation.

Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig (2005)
Journal Article
McGivan, J. D., Skinner, T. M., Doran (nee Udovikova), O., Haley, C. S., & Archibald, A. L. (2005). Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig. Animal Genetics, 36(5), 417-422. https://doi.org/10.1111/j.1365-2052.2005.01342.x

The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In a... Read More about Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig.

The pig CYP2E1 promoter is activated by COUP-TF1 and HNF-1 and is inhibited by androstenone (2004)
Journal Article
Doran, E., Tambyrajah, W. S., Doran (nee Udovikova), O., Wood, J. D., & McGivan, J. D. (2004). The pig CYP2E1 promoter is activated by COUP-TF1 and HNF-1 and is inhibited by androstenone. Archives of Biochemistry and Biophysics, 431(2), 252-260. https://doi.org/10.1016/j.abb.2004.08.016

Functional analysis of the pig cytochrome P4502E1 (CYP2E1) promoter identified two major activating elements. One corresponded to the hepatic nuclear factor 1 (HNF-1) consensus binding sequence at nucleotides -128/-98 and the other was located in the... Read More about The pig CYP2E1 promoter is activated by COUP-TF1 and HNF-1 and is inhibited by androstenone.

Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes (2002)
Journal Article
Wood, J. D., Doran (nee Udovikova), O., Whittington, F., & McGivan, J. D. (2002). Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes. Chemico-Biological Interactions, 140(1), 81-92. https://doi.org/10.1016/S0009-2797%2802%2900015-7

Skatole, a derivative of tryptophan, is produced in the hind-gut of pigs and is metabolised via hepatic cytochrome P4502E1 (CYP2E1). Excessive accumulation of skatole together with androstenone, a metabolite of testosterone, in adipose tissue in some... Read More about Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes.

Diagnostic assay for boar taint (2002)
Patent
McGivan, J. D., Wood, J. D., Whittington, F. M., Doran (nee Udovikova), O., & Meat and Livestock Commission, N. (2002). Diagnostic assay for boar taint. WO/2002/024945

(EN) The present invention discloses a link between the level of cytochrome P450 isoform P4502E1 and the level of skatole. A polynucleotide encoding the sequence for P4502E1 and a polynucleotide encoding the sequence for P4502E1 and a polynucleotide... Read More about Diagnostic assay for boar taint.

The relationship between adipose tissue skatole levels, rates of hepatic microsomal skatole metabolism and hepatic cytochrome P450IIE1 expression in two breeds of pig (2002)
Journal Article
Doran (nee Udovikova), O., Whittington, F., Wood, J. D., & McGivan, J. D. (2002). The relationship between adipose tissue skatole levels, rates of hepatic microsomal skatole metabolism and hepatic cytochrome P450IIE1 expression in two breeds of pig. Animal Science, 74(3), 461-468

The relationships between the level of skatole in backfat, the rate of skatole metabolism in isolated liver microsomes, hepatic cytochrome P450IIE1 content and mRNA levels were investigated in Large White ✕ Landrace (LW) and the Meishan ✕ Landrace... Read More about The relationship between adipose tissue skatole levels, rates of hepatic microsomal skatole metabolism and hepatic cytochrome P450IIE1 expression in two breeds of pig.

Mitochondrial aldehyde reductase: Identification and characterization in rat liver and kidney cortex (1998)
Journal Article
Udovikova, E. A., Doran (nee Udovikova), O., & Wojtzcak, L. (1998). Mitochondrial aldehyde reductase: Identification and characterization in rat liver and kidney cortex. International Journal of Biochemistry and Cell Biology, 30(5), 597-608. https://doi.org/10.1016/S1357-2725%2897%2900143-X

Aldehyde reductase (EC 1.1.1.2) has been regarded so far as an exclusively cytosolic enzyme. The present investigation shows that mitochondria of rat liver, kidney cortex and, tentatively, heart also contain an enzyme catalyzing oxidation of NADPH by... Read More about Mitochondrial aldehyde reductase: Identification and characterization in rat liver and kidney cortex.

The effect of osmotic pressure on oligomycin-sensitive ATPase activity and the liberation of Mg2+ from liver mitochondria of rats of various ages (1992)
Journal Article
Lemeshko, V. V., & Doran (nee Udovikova), O. (1992). The effect of osmotic pressure on oligomycin-sensitive ATPase activity and the liberation of Mg2+ from liver mitochondria of rats of various ages. Ukrainian Biochemical Journal, 61(4), 83-88

The influence of osmotic pressure of the incubating medium (25-500 mM sucrose) on oligomycin--sensitive, 2,4-dinitrophenyl-stimulated ATP-ase-activity, Mg2+ release and swelling of the liver mitochondria in 1-, 3-, 12-, 24-months Wistar rats is, inve... Read More about The effect of osmotic pressure on oligomycin-sensitive ATPase activity and the liberation of Mg2+ from liver mitochondria of rats of various ages.