Silvia I Nicolau-Solano
Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes
Nicolau-Solano, Silvia I; Doran, Olena
Abstract
Androstenone (5α-androst-16-en-3-one) is a steroid pheromone, excessive accumulation of which in pig adipose tissue contributes to the phenomenon of "boar taint". The incidence of boar taint increases with age and weight of entire male pigs. One of the reasons for the excessive accumulation of androstenone in adipose tissue of some pigs is a low rate of androstenone degradation in pig liver, which is related to defective expression of the androstenone-metabolising enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD). The mechanism regulating 3β-HSD expression in pig liver remains unclear. The present study investigated the effects of the sex steroids testosterone, estrone sulphate and androstenone on the expression of 3β-HSD protein using primary cultured hepatocytes as a model system. The study was performed on hepatocytes from pigs of two weight/age groups: "lighter weight" (average carcass weight 70kg, age 21weeks) and "heavier weight" (average carcass weight 92kg, age 26weeks). Testosterone at the range of concentrations from 5 to 100nM induced 3β-HSD expression in "lighter weight" pigs but not in "heavier weight" animals. In contrast, estrone sulphate (5 to 100nM) and androstenone (5 to 500nM) induced 3β-HSD expression only in "heavier weight" but not "lighter weight" pigs. Induction of 3β-HSD expression by the sex steroids was protein-synthesis-dependent. Overall this study demonstrates for the first time that sex steroids are involved in regulation of 3β-HSD protein expression in primary cultured pig hepatocytes and that the effect of sex steroids depends on age and weight of animals. The results of this work might contribute to understanding the mechanism controlling androstenone deposition in pigs. © 2007 Elsevier B.V. All rights reserved.
Citation
Nicolau-Solano, S. I., & Doran, O. (2008). Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes. Livestock Science, 114(2-3), 202-210. https://doi.org/10.1016/j.livsci.2007.05.001
Journal Article Type | Article |
---|---|
Publication Date | Apr 1, 2008 |
Journal | Livestock Science |
Print ISSN | 1871-1413 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 114 |
Issue | 2-3 |
Pages | 202-210 |
DOI | https://doi.org/10.1016/j.livsci.2007.05.001 |
Keywords | steroids, protein expression, primary cultured hepatocytes, 3-beta hydroxysteroid dehydrogenase |
Public URL | https://uwe-repository.worktribe.com/output/1020678 |
Publisher URL | http://dx.doi.org/10.1016/j.livsci.2007.05.001 |
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