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The manufacturing and characterisation of eugenol-enclosed liposomes produced by microfluidic method

Ghodke, Jessica; Ekonomou, Sotirios I.; Weaver, Edward; Lamprou, Dimitrios; Doran, Olena; Stratakos, Alexandros Ch.

The manufacturing and characterisation of eugenol-enclosed liposomes produced by microfluidic method Thumbnail


Authors

Jessica Ghodke

Sotirios I. Ekonomou

Edward Weaver

Dimitrios Lamprou

Olena Doran Olena.Doran@uwe.ac.uk
College Dean of Research and Enterprise

Alexandros Ch. Stratakos



Abstract

In this study, liposomes enclosing eugenol were prepared using microfluidics. Two lipids—1,2-dimyristoyl-sn-glycero-3-phosphocholine, 18:0 (DSPC) and 2-dimyristoyl-sn-glycero-3-phosphocholine, 14:0 (DMPC)—and microfluidic chips with serpentine and Y-shaped micromixing designs were used for the liposomal formulation. Minimum bactericidal concentration (MBC) values indicated that eugenol was more effective against Gram-negative than Gram-positive bacteria. Four different flow-rate ratios (FRR 2:1, 3:1, 4:1, 5:1) were explored. All liposomes’ encapsulation efficiency (EE) was determined: 94.34% for DSPC 3:1 and 78.63% for DMPC 5:1. The highest eugenol release of 99.86% was observed at pH 4, DMPC 3:1 (Y-shaped chip). Liposomes were physically stable at 4, 20 and 37 °C for 60 days as determined by their size, polydispersity index (PDI) and zeta potential (ZP). The most stable liposomes were observed at FRR 5:1 for DSPC. EE, stability, and eugenol release studies proved that the liposomal formulations produced can be used as delivery vehicles to increase food safety.

Citation

Ghodke, J., Ekonomou, S. I., Weaver, E., Lamprou, D., Doran, O., & Stratakos, A. C. (2023). The manufacturing and characterisation of eugenol-enclosed liposomes produced by microfluidic method. Foods, 12(15), Article 2940. https://doi.org/10.3390/foods12152940

Journal Article Type Article
Acceptance Date Aug 1, 2023
Online Publication Date Aug 3, 2023
Publication Date Aug 3, 2023
Deposit Date Aug 30, 2023
Publicly Available Date Sep 5, 2023
Journal Foods
Electronic ISSN 2304-8158
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 12
Issue 15
Article Number 2940
DOI https://doi.org/10.3390/foods12152940
Keywords liposomes, emerging technologies, food safety, storage stability, eugenol, microfluidics
Public URL https://uwe-repository.worktribe.com/output/11067652

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