Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig

McGivan, J. D.; Doran (nee Udovikova), Olena; Skinner, T. M.; Haley, C. S.; Archibald, A. L.

Abstract

The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross. © 2005 International Society for Animal Genetics.

Citation

McGivan, J. D., Doran, E., Skinner, T. M., Doran (nee Udovikova), O., McGivan, J. D., Haley, C. S., & Archibald, A. L. (2005). Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig. Animal Genetics, 36(5), 417-422. https://doi.org/10.1111/j.1365-2052.2005.01342.x

• Journal Article Type: Article
• Publication Date: Oct 1, 2005
• Journal: Animal Genetics
• Print ISSN: 0268-9146
• Electronic ISSN: 1365-2052
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 36
• Issue: 5
• Pages: 417-422
• DOI: https://doi.org/10.1111/j.1365-2052.2005.01342.x
• Keywords: CYP2E1 gene, skatole, cloning, polymoprhisms
• Public URL: https://uwe-repository.worktribe.com/output/1056180
• Publisher URL: http://dx.doi.org/10.1111/j.1365-2052.2005.01342.x
• Additional Information: Corporate Creators : University of Bristol, ROslin Insitute