Relationship between the expression of hepatic but not testicular 3β-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue

McGivan, J. D.; Whittington, F. M.; Wood, J. D.; Nicolau-Solano, Silvia I; Nieuwhof, Gert J; Doran (nee Udovikova), Olena

Abstract

This study investigated the relationship between expression of hepatic and testicular 3/3-hydroxysteroid dehydrogenase (3/3-HSD) and accumulation of androstenone in adipose tissue because of its relation to boar taint. The experiments were performed on 13 Large White (50%) × Landrace (50%) and Meishan (25%) × Large White (25%) × Landrace (50%), pigs, which differed in the level of backfat androstenone. Our previous work showed that the major product of the hepatic androstenone metabolism is 3β-androstenol. In this study, the formation of 3β-androstenol was inhibited by the specific 3β-HSD inhibitor trilostane. These results are the first direct confirmation that 3β-HSD is the enzyme responsible for androstenone metabolism in the pig. The expression of the hepatic but not testicular 3β-HSD protein showed a negative relationship with the level of backfat androstenone (r2 = 0.64; P < 0.001) and was accompanied by a reduced rate of the hepatic androstenone clearance. Low expression of 3β-HSD protein in the liver of high androstenone pigs was also accompanied by a reduced level of 3β-HSD mRNA (P < 0.001), which suggests a defective regulation of the hepatic 3β-HSD expression at the level of transcription. In contrast, expression of the testicular 3β-HSD protein did not differ between animals with high and low androstenone levels (P > 0.05) and was lower compared with the hepatic 3β-HSD expression. Cloning and sequencing of the 3β-HSD coding regions established that the hepatic and testicular 3β-HSD cDNA have identical sequences, which were 98% similar to the human 3β-HSD isoform I. It is suggested that expression of a single 3β-HSD gene is regulated by different mechanisms in pig liver and testis. The liver-specific regulation of 3β-HSD expression contributes to the low rate of hepatic androstenone metabolism and therefore can be considered as one of the factors regulating deposition of androstenone in pig adipose tissue and subsequent development of boar taint. ©2006 American Society of Animal Science. All rights reserved.

• Journal Article Type: Article
• Publication Date: Oct 1, 2006
• Deposit Date: Jun 7, 2011
• Journal: Journal of Animal Science
• Print ISSN: 0021-8812
• Electronic ISSN: 1525-3163
• Publisher: Oxford University Press (OUP)
• Peer Reviewed: Peer Reviewed
• Volume: 84
• Issue: 10
• Pages: 2809-2817
• DOI: https://doi.org/10.2527/jas.2005-595
• Keywords: food qaulity, androstenone, boar taint, 3β-hydroxysteroid dehydrogenase, pig
• Public URL: https://uwe-repository.worktribe.com/output/1044845
• Publisher URL: http://dx.doi.org/10.2527/jas.2005-595
• Additional Information: Corporate Creators : University of Bristol, Meat and Livestock Commission
• Contract Date: Apr 26, 2016