All Outputs (74)

Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations (2014)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2014). Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations. https://doi.org/10.5640/insc.040101

Benzodiazepines are an important class of drugs commonly administered with a potential for abuse and environmental pollution. This review focuses on the liquid chromatographic electrochemical detection of the benzodiazepine class of drugs. These are... Read More about Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations.

Use of whole blood for analysis of disease-associated biomarkers (2013)
Journal Article
McLeod, J., Pemberton, R. M., May, J. E., Pemberton, R., Hart, J. P., McLeod, J. D., …Doran, O. (2013). Use of whole blood for analysis of disease-associated biomarkers. Analytical Biochemistry, 437(1), 59-61. https://doi.org/10.1016/j.ab.2013.02.024

Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of... Read More about Use of whole blood for analysis of disease-associated biomarkers.

Microfabricated glucose biosensor for culture welloperation (2013)
Journal Article
Hart, J. P., Luxton, R., Xu, J., Griffiths, J., Sage, I., Tuffin, R., …Hart, J. P. (2013). Microfabricated glucose biosensor for culture welloperation. Biosensors and Bioelectronics, 42(1), 668-677. https://doi.org/10.1016/j.bios.2012.11.032

A water-based carbon screen-printing ink formulation, containing the redox mediator cobalt phthalocyanine (CoPC) and the enzyme glucose oxidase (GOx), was investigated for its suitability to fabricate glucose microbiosensors in a 96-well microplate f... Read More about Microfabricated glucose biosensor for culture welloperation.

Development of an amperometric screen-printed galactose biosensor for serum analysis (2013)
Journal Article
Pemberton, R. M., Kanyong, P., Pemberton, R., Jackson, S. K., & Hart, J. P. (2013). Development of an amperometric screen-printed galactose biosensor for serum analysis. Analytical Biochemistry, 435(2), 114-119. https://doi.org/10.1016/j.ab.2013.01.006

The development of a disposable amperometric biosensor for the measurement of circulating galactose in serum is described. The biosensor comprises a screen-printed carbon electrode (SPCE), incorporating the electrocatalyst cobalt phthalocyanine (CoPC... Read More about Development of an amperometric screen-printed galactose biosensor for serum analysis.

The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay (2013)
Journal Article
Crew, A., Honeychurch, K. C., Crew, A. P., Northall, H., Radbourne, S., Davies, O., …Hart, J. P. (2013). The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay. Talanta, 116, 300-307. https://doi.org/10.1016/j.talanta.2013.05.017

In this study we investigated the possibility of applying disposable electrochemical screen-printed carbon sensors for the rapid identification and quantitative determination of diazepam in beverages. This was achieved utilising a previously unreport... Read More about The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay.

The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine (2012)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2012). The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine. Advances in Analytical Chemistry, 2(5), 46-52. https://doi.org/10.5923/j.aac.20120205.01

Screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) have been successfully used for the manufacture of microband electrodes for the determination of reduced glutathione (GSH). Initially investigati... Read More about The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine.

Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis (2012)
Journal Article
Kanyong, P., Pemberton, R., Jackson, S. K., & Hart, J. P. (2012). Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis. Analytical Biochemistry, 428(1), 39-43. https://doi.org/10.1016/j.ab.2012.05.027

A screen-printed carbon electrode (SPCE) incorporating the electrocatalyst cobalt phthalocyanine (CoPC), fabricated using a water-based ink formulation, has been investigated as the base transducer for a uric acid biosensor. A sandwich biosensor was... Read More about Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.

Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum (2012)
Journal Article
Honeychurch, K. C., Chong, A. T., Elamin, K., & Hart, J. P. (2012). Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum. Analytical Methods, 4(1), 132-140. https://doi.org/10.1039/c1ay05419h

Previously unreported voltammetric redox behaviour was identified for flunitrazepam, lorazepam and diazepam at a glassy carbon electrode. This was successfully exploited for their determination in serum by high performance liquid chromatography dual... Read More about Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum.

Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples (2011)
Journal Article
Jenkins, A. T., Gilbert, L., Browning, S., & Hart, J. P. (2011). Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples. Sensors and Actuators B: Chemical, 160(1), 1322-1327. https://doi.org/10.1016/j.snb.2011.09.069

An amperometric phosphate biosensor, based on a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) is described. The immobilisation of the enzyme pyruvate oxidase (PyOd) was investigated using pre-formed cellulose acetate/cellulose nit... Read More about Development of an amperometric, screen-printed, single-enzyme phosphate ion biosensor and its application to the analysis of biomedical and environmental samples.

Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection (2011)
Journal Article
Kumar, A., Hart, J. P., & McCalley, D. V. (2011). Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection. Journal of Chromatography A, 1218(25), 3854-3861. https://doi.org/10.1016/j.chroma.2011.04.034

The determination of catecholamines in urine was investigated using hydrophilic interaction chromatography (HILIC) as an alternative to the commonly used reversed-phase (RP) method. A number of different approaches were explored, including per-aqueou... Read More about Determination of catecholamines in urine using hydrophilic interaction chromatography with electrochemical detection.

The voltammetric behaviour of lead at a microband screen-printed carbon electrode and its determination in acetate leachates from glazed ceramic plates (2011)
Journal Article
Honeychurch, K. C., Al-Berezanchi, S., & Hart, J. P. (2011). The voltammetric behaviour of lead at a microband screen-printed carbon electrode and its determination in acetate leachates from glazed ceramic plates. Talanta, 84(3), 717-723. https://doi.org/10.1016/j.talanta.2011.01.073

Microband screen-printed carbon electrodes (μBSPCEs) without further modification have been investigated as disposable sensors for the measurement of lead in acetate leachates from ceramic glazed plates. Cyclic voltammetry was employed to elucidate t... Read More about The voltammetric behaviour of lead at a microband screen-printed carbon electrode and its determination in acetate leachates from glazed ceramic plates.

A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides (2011)
Journal Article
Crew, A. P., Lonsdale, D., Byrd, N., Pittson, R., & Hart, J. P. (2011). A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides. Biosensors and Bioelectronics, 26(6), 2847-2851. https://doi.org/10.1016/j.bios.2010.11.018

Organophosphate pesticides present serious risks to human and environmental health. A rapid reliable, economical and portable analytical system will be of great benefit in the detection and prevention of contamination. A biosensor array based on six... Read More about A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides.

A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture (2011)
Journal Article
Xu, J., Pemberton, R., Pittson, R., Drago, G. A., Griffiths, J., Jackson, S. K., & Hart, J. P. (2011). A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture. Biosensors and Bioelectronics, 26(5), 2448-2453. https://doi.org/10.1016/j.bios.2010.10.030

Microband biosensors, screen-printed from a water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, were used to monitor glucose levels continuously in buffer and culture medium. Five biosensors were o... Read More about A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture.

A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture (2011)
Journal Article
Pemberton, R., Xu, J., Pittson, R., Drago, G., Griffiths, J., Jackson, S. K., & Hart, J. P. (2011). A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture. Biosensors and Bioelectronics, 26(5), 2448-2453. https://doi.org/10.1016/j.bios.2010.10.030

#Microband biosensors, screen-printed from a water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, were used to monitor glucose levels continuously in buffer and culture medium. Five biosensors were... Read More about A screen-printed microband glucose biosensor system for real-time monitoring of toxicity in cell culture.

Voltammetric behavior of DNA and its derivatives using screen printed carbon electrodes and its possible application in genotoxicity screening (2010)
Journal Article
Rawson, F. J., Jackson, S. K., & Hart, J. P. (2010). Voltammetric behavior of DNA and its derivatives using screen printed carbon electrodes and its possible application in genotoxicity screening. Analytical Letters, 43(10-11), 1790-1800. https://doi.org/10.1080/00032711003653908

The aim of the current work was to characterize the electrochemical behavior of DNA and its derivatives using screen printed carbon electrodes (SPCEs). Differential pulse voltammetry (DPV) was carried out on guanine, adenine, 8-oxyguanine, 8-deoxygau... Read More about Voltammetric behavior of DNA and its derivatives using screen printed carbon electrodes and its possible application in genotoxicity screening.

Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+ (2010)
Journal Article
Piano, M., Serban, S., Pittson, R., Drago, G. A., & Hart, J. P. (2010). Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+. Talanta, 82(1), 34-37. https://doi.org/10.1016/j.talanta.2010.03.051

A biosensor for the measurement of lactate in serum has been developed, which is based on a screen-printed carbon electrode, modified with Meldola's Blue-Reinecke Salt (MBRS-SPCE), coated with the enzyme lactate dehydrogenase NAD+ dependent (from Por... Read More about Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+.

Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine- modified screen-printed carbon electrode and its application in pharmaceutical and food analysis (2010)
Journal Article
Honeychurch, K. C., Gilbert, L., & Hart, J. P. (2010). Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine- modified screen-printed carbon electrode and its application in pharmaceutical and food analysis. Analytical and Bioanalytical Chemistry, 396(8), 3103-3111. https://doi.org/10.1007/s00216-010-3534-x

Cobalt phthalocyanine-modified screen-printed carbon electrodes (CoPC-SPCEs) have been investigated as disposable sensors for the measurement of citric acid. The analyte was found to undergo an electrocatalytic oxidation process involving the Co2+/Co... Read More about Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine- modified screen-printed carbon electrode and its application in pharmaceutical and food analysis.

Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins (2010)
Journal Article
Pemberton, R., Rawson, F. J., Xu, J., Pittson, R., Drago, G. A., Griffiths, J., …Hart, J. P. (2010). Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins. Microchimica Acta, 170(3), 321-330. https://doi.org/10.1007/s00604-010-0326-0

Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferro... Read More about Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins.

Studies towards an amperometric phosphate ion biosensor for urine and water analysis (2010)
Journal Article
Jenkins, A. T., Gilbert, L., Browning, S., & Hart, J. P. (2010). Studies towards an amperometric phosphate ion biosensor for urine and water analysis. Microchimica Acta, 170(3), 331-336. https://doi.org/10.1007/s00604-010-0316-2

An amperometric biosensor for phosphate ion is described that is based on a cobalt phthalocyanine modified screen-printed carbon electrode (CoPC-SPCE). The biosensor operation is based on the enzyme pyruvate oxidase (PyOd) which catalyses the oxidati... Read More about Studies towards an amperometric phosphate ion biosensor for urine and water analysis.

A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose (2010)
Journal Article
Piano, M., Serban, S., Biddle, N., Pittson, R., Drago, G. A., & Hart, J. P. (2010). A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose. Analytical Biochemistry, 396(2), 269-274. https://doi.org/10.1016/j.ab.2009.09.028

A biosensor for the measurement of glucose in serum has been developed, based on a screen-printed carbon electrode modified with Meldola's Blue-Reinecke salt, coated with the enzyme glucose dehydrogenase (from Bacillus sp.), and nicotinamide adenine... Read More about A flow injection system, comprising a biosensor based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt coated with glucose dehydrogenase, for the measurement of glucose.