Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+

Piano, M.; Serban, S.; Pittson, R.; Drago, G. A.; Hart, John P.

Abstract

A biosensor for the measurement of lactate in serum has been developed, which is based on a screen-printed carbon electrode, modified with Meldola's Blue-Reinecke Salt (MBRS-SPCE), coated with the enzyme lactate dehydrogenase NAD+ dependent (from Porcine heart), and NAD+. A cellulose acetate layer was deposited on the top of the device to act as a permselective membrane. The biosensor was incorporated into a commercially available, thin-layer, amperometric flow cell operated at a potential of only +0.05 V vs. Ag/AgCl. The mobile phase consisted of 0.2 M phosphate buffer pH 10 containing 0.1 M potassium chloride solution; a flow rate of 0.8 ml min-1 was used throughout the investigation. The biosensor response was linear over the range 0.55-10 mM lactate; the former represents the detection limit. The precision of the system was determined by carrying out 10 repeat injections of 10 mM l(+)lactic acid standard; the calculated coefficient of variation was 4.28%. It was demonstrated that this biosensor system could be applied to the direct measurement of lactate in serum without pre-treatment; therefore, this would allow high throughput-analysis, at low cost, for this clinically important analyte. © 2010 Elsevier B.V. All rights reserved.

• Journal Article Type: Article
• Publication Date: Jun 30, 2010
• Journal: Talanta
• Print ISSN: 0039-9140
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 82
• Issue: 1
• Pages: 34-37
• DOI: https://doi.org/10.1016/j.talanta.2010.03.051
• Keywords: flow cell, screen-printed electrode, Meldola's Blue-Reinecke salt, amperometric biosensor, L(+) lactate, serum, FIA
• Public URL: https://uwe-repository.worktribe.com/output/978783
• Publisher URL: http://dx.doi.org/10.1016/j.talanta.2010.03.051