Facile and inexpensive fabrication of zinc oxide based bio-surfaces for C-reactive protein detection

Lu, Cao; Janice, Kiely; Martina, Piano; Richard, Luxton

Abstract

© 2018, The Author(s). The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using a rapid and inexpensive fabrication method which utilises colloidal dispersion enhanced using sonication. This technique was used to prepare highly ordered and uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) using 0.5%, 1% and 5% concentrations of zinc oxide nano-crystal suspensions. Impedance spectroscopy was employed to interrogate the sensor surfaces and confirmed high reproducibility of the fabrication process. Changes in impedance values, at a frequency of 138 Hz, were used to establish dose dependent responses for C-reactive protein (CRP) antigen. A limit of detection of less than 1 ng/ml was demonstrated fornano-surfaces fabricated from concentrations of 1% ZnO.

• Journal Article Type: Article
• Acceptance Date: Aug 2, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Sep 6, 2018
• Publicly Available Date: Sep 6, 2018
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Research (part of Springer Nature)
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 1
• Pages: 122687
• DOI: https://doi.org/10.1038/s41598-018-30793-z
• Keywords: biosenor, zinc oxide, rapid, nanomaterials,
• Public URL: https://uwe-repository.worktribe.com/output/862678
• Publisher URL: http://dx.doi.org/10.1038/s41598-018-30793-z
• Contract Date: Sep 6, 2018