The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine

Honeychurch, Kevin C.; Hart, John P.

Abstract

Screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) have been successfully used for the manufacture of microband electrodes for the determination of reduced glutathione (GSH). Initially investigations were performed to explore the cyclic voltammetric behaviour of these electrodes with potassium ferrocyanide. It was shown that steady state behaviour occurred which is indicative of radial diffusion at a microelectrode. Current densities of 2173 μAcm-2 were obtained compared to 464.4 μAcm-2 for conventional sized (3 mm x 3 mm) screen-printed electrodes were obtained. At scan rates between 5 and 20 mV/s no significant increase in current response was observed. The application of these microband CoPC–SPCEs, to the measurement of GSH by both chronoamperometry and cyclic voltammetry was investigated. The chronoamperometric response was found to be linear between 50 µM and 8.0 mM with a detection limit of 7.3 µM using an applied potential of +0.25 V (vs. Ag/AgCl).

• Journal Article Type: Article
• Publication Date: Nov 5, 2012
• Deposit Date: Nov 21, 2012
• Publicly Available Date: Nov 15, 2016
• Journal: Advances in Analytical Chemistry
• Print ISSN: 2163-1557
• Publisher: Hans Publishers
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 5
• Pages: 46-52
• DOI: https://doi.org/10.5923/j.aac.20120205.01
• Keywords: glutathione, cobalt phthalocyanine screen-printed carbon electrodes, voltammetry, chronoamperometry, microband electrodes
• Public URL: https://uwe-repository.worktribe.com/output/942111
• Publisher URL: http://article.sapub.org/10.5923.j.aac.20120205.01.html
• Contract Date: Nov 15, 2016