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Use of whole blood for analysis of disease-associated biomarkers

Pemberton, Roy M.; McLeod, Julie; Pemberton, Roy; May, Jennifer E.; McLeod, Julie D.; Hart, John P.; Wilcock, Gordon; Doran, Olena


Roy M. Pemberton

Julie McLeod

Jennifer May
Senior Lecturer in Biomedical Science

Julie D. McLeod

Gordon Wilcock


Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of plasma or serum. We present a feasibility study demonstrating potential use of fresh or frozen whole blood to detect soluble biomarkers using an enzyme-linked immunosorbent assay (ELISA)-based method. Good correlation between levels of soluble CD25 in plasma and whole blood of healthy individuals or Alzheimer's patients was established. These results provide a basis for development of a novel biosensor approach for disease-associated biomarker detection in whole blood. © 2013 Elsevier Inc. All rights reserved.


McLeod, J., Pemberton, R. M., May, J. E., Pemberton, R., Hart, J. P., McLeod, J. D., …Doran, O. (2013). Use of whole blood for analysis of disease-associated biomarkers. Analytical Biochemistry, 437(1), 59-61.

Journal Article Type Article
Publication Date Jun 1, 2013
Journal Analytical Biochemistry
Print ISSN 0003-2697
Electronic ISSN 1096-0309
Publisher Elsevier Masson
Peer Reviewed Peer Reviewed
Volume 437
Issue 1
Pages 59-61
Keywords biomarkers, whole blood, plasma, ELISA, CD25
Public URL
Publisher URL
Additional Information Additional Information : published online ahead of print


Analytical Biochem Accepted manuscript (inc figures).docx (262 Kb)

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