All Outputs (36)

A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana (2002)
Journal Article
Desikan, R., Griffiths, R., Hancock, J. T., & Neill, S. (2002). A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 99(25), 16314-16318. https://doi.org/10.1073/pnas.252461999

The plant hormone abscisic acid (ABA), synthesized in response to water-deficit stress, induces stomatal closure via activation of complex signaling cascades. Recent work has established that nitric oxide (NO) is a key signaling molecule mediating AB... Read More about A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana.

Highly sensitive mixed oxide sensors for the detection of ethanol (2002)
Journal Article
de Lacy Costello, B., Ewen, R., Guernion, N., & Ratcliffe, N. M. (2002). Highly sensitive mixed oxide sensors for the detection of ethanol. Sensors and Actuators B: Chemical, 87(1), 207-210. https://doi.org/10.1016/S0925-4005%2802%2900220-4

Sensors consisting of mixtures of tin dioxide and zinc oxide powders in a range of proportions were constructed. Each mixture was applied to an electrode-bearing alumina substrate either as a paste, or by screen printing. The responses of these senso... Read More about Highly sensitive mixed oxide sensors for the detection of ethanol.

Multifunctional zinc finger proteins in development and disease (2002)
Journal Article
Ladomery, M., & Dellaire, G. (2002). Multifunctional zinc finger proteins in development and disease. Annals of Human Genetics, 66(5-6), 331-342. https://doi.org/10.1046/j.1469-1809.2002.00121.x

Post-transcriptional processes contribute significantly towards the generation of proteomic diversity. An increasing number of mutations have been described that affect genes encoding components of the post-transcriptional machinery. In particular, m... Read More about Multifunctional zinc finger proteins in development and disease.

Experimental logical gates in a reaction-diffusion medium: The XOR gate and beyond (2002)
Journal Article
Adamatzky, A., & de Lacy Costello, B. (2002). Experimental logical gates in a reaction-diffusion medium: The XOR gate and beyond. Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics, 66(4), 6. https://doi.org/10.1103/PhysRevE.66.046112

We exploit the particulars of diffusive wave front interactions in certain types of two-reactant reaction-diffusion medium to construct a laboratory prototype of an XOR gate. In the design, the values of the logic variables are represented by the pre... Read More about Experimental logical gates in a reaction-diffusion medium: The XOR gate and beyond.

Collision-free path planning in the Belousov-Zhabotinsky medium assisted by a cellular automaton (2002)
Journal Article
Adamatzky, A., & de Lacy Costello, B. (2002). Collision-free path planning in the Belousov-Zhabotinsky medium assisted by a cellular automaton. Naturwissenschaften, 89(10), 474-478. https://doi.org/10.1007/s00114-002-0363-6

We offer a new approach to computing a shortest collision-free path in a space containing obstacles, using an experimental chemical processor, based on the Belousov-Zhabotinsky (BZ) reaction. The chemical processor was then coupled via optical links... Read More about Collision-free path planning in the Belousov-Zhabotinsky medium assisted by a cellular automaton.

Antimicrobial properties of milk: Dependence on presence of xanthine oxidase and nitrite (2002)
Journal Article
Hancock, J. T., Salisbury, V., Ovejero-Boglione, M. C., Cherry, R., Hoare, C., Eisenthal, R., & Harrison, R. (2002). Antimicrobial properties of milk: Dependence on presence of xanthine oxidase and nitrite. Antimicrobial Agents and Chemotherapy, 46(10), 3308-3310. https://doi.org/10.1128/AAC.46.10.3308-3310.2002

Human and bovine milk inhibited the metabolic activity of Escherichia coli, as shown by luminescence monitoring of constructs expressing the luxCDABE genes. Inhibition was dependent on both xanthine oxidase (XO) activity and on the presence of nitrit... Read More about Antimicrobial properties of milk: Dependence on presence of xanthine oxidase and nitrite.

Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms (2002)
Journal Article
Yennawar, N. H., Conway, M. E., Yennawar, H. P., Farber, G. K., & Hutson, S. M. (2002). Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms. Biochemistry, 41(39), 11592-11601. https://doi.org/10.1021/bi020221c

The three-dimensional structures of the isoleucine ketimine and the pyridoxamine phosphate forms of human mitochondrial branched chain aminotransferase (hBCATm) have been determined crystallographically at 1.9 Å resolution. The hBCATm-catalyzed trans... Read More about Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms.

Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases (2002)
Journal Article
Buckenmaier, S. M., McCalley, D. V., & Euerby, M. R. (2002). Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases. Analytical Chemistry, 74(18), 4672-4681. https://doi.org/10.1021/ac0202381

The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass are notably different when mobile-phase p... Read More about Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases.

Evaluation of HER-2/neu immunohistochemical assay sensitivity and scoring on formalin-fixed and paraffin-processed cell lines and breast tumors: A comparative study involving results from laboratories in 21 countries (2002)
Journal Article
Rhodes, A., Jasani, B., Anderson, E., Dodson, A. R., & Balaton, A. J. (2002). Evaluation of HER-2/neu immunohistochemical assay sensitivity and scoring on formalin-fixed and paraffin-processed cell lines and breast tumors: A comparative study involving results from laboratories in 21 countries. American Journal of Clinical Pathology, 118(3), 408-417. https://doi.org/10.1309/97WN-W6UX-XJWT-02H2

Variation in assay sensitivity was studied in more than 90 laboratories that assayed 4formalin-fixed, paraffin-processed breast and ovarian carcinoma cell lines with graded levels of HER-2/neu protein overexpression and known levels of HER-2/neu gene... Read More about Evaluation of HER-2/neu immunohistochemical assay sensitivity and scoring on formalin-fixed and paraffin-processed cell lines and breast tumors: A comparative study involving results from laboratories in 21 countries.

Concentrations of Cd, Zn and Cu in sediments and brown shrimp (Crangon crangon L.) from the Severn Estuary and Bristol Channel, UK (2002)
Journal Article
Culshaw, C., Newton, L. C., Weir, I., & Bird, D. J. (2002). Concentrations of Cd, Zn and Cu in sediments and brown shrimp (Crangon crangon L.) from the Severn Estuary and Bristol Channel, UK. Marine Environmental Research, 54(3-5), 331-334. https://doi.org/10.1016/S0141-1136%2802%2900163-0

Cd, Zn and Cu levels were determined in sediments and Crangon crangon from 9 sites in the Severn Estuary/Bristol Channel during winter 1999. Metal levels in both shrimp and sediments varied significantly between sites and were related to proximity of... Read More about Concentrations of Cd, Zn and Cu in sediments and brown shrimp (Crangon crangon L.) from the Severn Estuary and Bristol Channel, UK.

A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases (2002)
Journal Article
Cooper, A. J. L., Conway, M., & Hutson, S. M. (2002). A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases. Analytical Biochemistry, 308(1), 100-105. https://doi.org/10.1016/S0003-2697%2802%2900243-9

A new, continuous 96-well plate spectrophotometric assay for the branched-chain amino acid aminotransferases is described. Transamination of L-leucine with α-ketoglutarate results in formation of α-ketoisocaproate, which is reductively aminated back... Read More about A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases.

The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy (2002)
Journal Article
Reynolds, D. M. (2002). The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy. Journal of Chemical Technology and Biotechnology, 77(8), 965-972. https://doi.org/10.1002/jctb.664

The chemical and biochemical oxygen demand values of a number of synthetic and wastewater samples were determined using fluorescence spectroscopy. Treated and untreated sewage samples were obtained from a local sewage treatment works while synthetic... Read More about The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy.

Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase (2002)
Journal Article
Conway, M. E., Yennawar, N., Wallin, R., Poole, L. B., & Hutson, S. M. (2002). Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase. Biochemistry, 41(29), 9070-9078. https://doi.org/10.1021/bi020200i

The human mitochondrial branched chain aminotransferase isoenzyme (hBCATm) must be stored in a reducing environment to remain active. Oxidation or labeling of hBCATm with sulfhydryl reagents results in enzyme inhibition. In this study, we investigate... Read More about Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase.

Phytoextractio of 137Cs: The effect of soil 137Cs concentration on 137Cs uptake by Beta vulgaris (2002)
Journal Article
Watt, N. R., Willey, N., Hall, S. C., & Cobb, A. (2002). Phytoextractio of 137Cs: The effect of soil 137Cs concentration on 137Cs uptake by Beta vulgaris. Acta Biotechnologica, 22(1-2), 183-188. https://doi.org/10.1002/1521-3846%28200205%2922%3A1/2%3C183%3A%3AAID-ABIO183%3E3.0.CO%3B2-K

The last phase for MAGNOX ELECTRIC plc's reactor decommissioning strategy is site clearance and delicencing. Phytoextraction of 137Cs may be able to assist in meeting this objective. Field trials using Beta vulgaris conducted at Bradwell Nuclear Powe... Read More about Phytoextractio of 137Cs: The effect of soil 137Cs concentration on 137Cs uptake by Beta vulgaris.

Cell signalling following plant/pathogen interactions involves the generation of reactive oxygen and reactive nitrogen species (2002)
Journal Article
Hancock, J. T., Desikan, R., Clarke, A., Hurst, R. D., & Neill, S. (2002). Cell signalling following plant/pathogen interactions involves the generation of reactive oxygen and reactive nitrogen species. Plant Physiology and Biochemistry, 40(6-8), 611-617. https://doi.org/10.1016/S0981-9428%2802%2901403-1

It is now clear that reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), and reactive nitrogen species, such as nitric oxide (NO), are produced by plant cells in response to a variety of stresses, including pathogen challenge. Such molec... Read More about Cell signalling following plant/pathogen interactions involves the generation of reactive oxygen and reactive nitrogen species.

Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode (2002)
Journal Article
Honeychurch, K. C., Hawkins, D. M., Hart, J. P., & Cowell, D. C. (2002). Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode. Talanta, 57(3), 565-574. https://doi.org/10.1016/S0039-9140%2802%2900060-7

Screen-printed carbon electrodes (SPCEs), without chemical modification, have been investigated as disposable sensors for the measurement of trace levels of Cu2+. Cyclic voltammetry was employed to elucidate the electrochemical behaviour of Cu2+ at t... Read More about Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode.

Experimental reaction-diffusion pre-processor for shape recognition (2002)
Journal Article
Adamatzky, A., de Lacy Costello, B., & Ratcliffe, N. M. (2002). Experimental reaction-diffusion pre-processor for shape recognition. Physics Letters A, 297(5-6), 344-352. https://doi.org/10.1016/S0375-9601%2802%2900289-X

We have produced an experimental implementation of a massively-parallel reaction-diffusion processor which performs one of the most essential parts of shape recognition - construction of a skeleton. A skeleton is a unique, stable and invariant repres... Read More about Experimental reaction-diffusion pre-processor for shape recognition.

Hydrogen peroxide and nitric oxide as signalling molecules in plants (2002)
Journal Article
Neill, S., Desikan, R., Clarke, A., Hurst, R. D., & Hancock, J. T. (2002). Hydrogen peroxide and nitric oxide as signalling molecules in plants. Journal of Experimental Botany, 53(372), 1237-1247

It is now clear that hydrogen peroxide (H2O2) and nitric oxide (NO) function as signalling molecules in plants. A wide range of abiotic and biotic stresses results in H2O2 generation, from a variety of sources. H2O2 is removed from cells via a number... Read More about Hydrogen peroxide and nitric oxide as signalling molecules in plants.

The synthesis of 3-octadecyl- and 3-docosylpyrrole, their polymerisation and incorporation into novel composite gas sensitive resistors (2002)
Journal Article
Ratcliffe, N. M., Guernion, N., & de Lacy Costello, B. (2002). The synthesis of 3-octadecyl- and 3-docosylpyrrole, their polymerisation and incorporation into novel composite gas sensitive resistors. Synthetic Metals, 128(2), 139-147. https://doi.org/10.1016/S0379-6779%2801%2900551-3

3-Octadecylpyrrole and the novel 3-docosylpyrrole were synthesised in high yields. These monomers were polymerised with various oxidising agents to produce a range of 3-octadecylpolypyrroles and 3-docosylpolypyrroles with different counter-ions. Thes... Read More about The synthesis of 3-octadecyl- and 3-docosylpyrrole, their polymerisation and incorporation into novel composite gas sensitive resistors.

Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes (2002)
Journal Article
Wood, J. D., Doran (nee Udovikova), O., Whittington, F., & McGivan, J. D. (2002). Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes. Chemico-Biological Interactions, 140(1), 81-92. https://doi.org/10.1016/S0009-2797%2802%2900015-7

Skatole, a derivative of tryptophan, is produced in the hind-gut of pigs and is metabolised via hepatic cytochrome P4502E1 (CYP2E1). Excessive accumulation of skatole together with androstenone, a metabolite of testosterone, in adipose tissue in some... Read More about Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes.