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Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Drago, Guido A.; Sage, Ian C.; Pemberton, Roy; Cox, Timothy; Tuffin, Rachel; Griffiths, John; Pittson, Robin; Johnson, Graham; Xu, Jinsheng; Luxton, Richard W.; Davies, Rhodri; Jackson, Simon K.; Kenna, Gerry; Hart, John P.

Authors

Guido A. Drago

Ian C. Sage

Rachel Tuffin

John Griffiths

Robin Pittson

Graham Johnson

Jinsheng Xu jinsheng.xu@uwe.ac.uk

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor

Rhodri Davies

Simon K. Jackson simon.jackson@uwe.ac.uk

Gerry Kenna



Abstract

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.

Journal Article Type Article
Publication Date Oct 30, 2014
Journal Sensors (Switzerland)
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 14
Issue 11
Pages 20519-20532
APA6 Citation Luxton, R., Sage, I. C., Drago, G. A., Pemberton, R. M., Pemberton, R., Cox, T., …Hart, J. P. (2014). Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers. Sensors, 14(11), 20519-20532. https://doi.org/10.3390/s141120519
DOI https://doi.org/10.3390/s141120519
Keywords microbiosensor, screen-printing, MEMS, 96-well plate, metabolism, amperometry, cell monitoring, toxicity testing
Publisher URL http://dx.doi.org/10.3390/s141120519

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