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Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Sage, Ian C.; Drago, Guido A.; Pemberton, Roy; Cox, Timothy; Tuffin, Rachel; Griffiths, John; Pittson, Robin; Johnson, Graham; Xu, Jinsheng; Davies, Rhodri; Jackson, Simon K.; Kenna, Gerry; Luxton, Richard W.; Hart, John P.


Ian C. Sage

Guido A. Drago

Rachel Tuffin

John Griffiths

Robin Pittson

Graham Johnson

Jinsheng Xu

Rhodri Davies

Simon K. Jackson

Gerry Kenna

Richard Luxton
Research Centre Director-IBST/Professor


© 2014 by the authors; licensee MDPI, Basel, Switzerland. This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.


Sage, I. C., Drago, G. A., Pemberton, R., Cox, T., Tuffin, R., Griffiths, J., …Hart, J. P. (2014). Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers. Sensors, 14(11), 20519-20532.

Journal Article Type Article
Acceptance Date Oct 21, 2014
Online Publication Date Oct 30, 2014
Publication Date Nov 1, 2014
Deposit Date Dec 15, 2014
Journal Sensors (Switzerland)
Electronic ISSN 1424-8220
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 14
Issue 11
Pages 20519-20532
Keywords microbiosensor, screen-printing, MEMS, 96-well plate, metabolism, amperometry, cell monitoring, toxicity testing
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