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An improved conjugation method for Pseudomonas syringae

Neale, Helen C.; Hulin, Michelle T.; Harrison, Richard J.; Jackson, Robert W.; Mansfield, John W.; Arnold, Dawn L.

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Authors

Helen Neale Helen2.Neale@uwe.ac.uk
Occasional Associate Lecturer - HAS DAS

Michelle T. Hulin

Richard J. Harrison

Robert W. Jackson

John W. Mansfield

Dawn Arnold Dawn.Arnold@uwe.ac.uk
Professor in Molecular Plant Pathology



Abstract

In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

Citation

Neale, H. C., Hulin, M. T., Harrison, R. J., Jackson, R. W., Mansfield, J. W., & Arnold, D. L. (2020). An improved conjugation method for Pseudomonas syringae. Journal of Microbiological Methods, 177, https://doi.org/10.1016/j.mimet.2020.106025

Journal Article Type Article
Acceptance Date Aug 5, 2020
Online Publication Date Aug 11, 2020
Publication Date Oct 1, 2020
Deposit Date Aug 26, 2020
Publicly Available Date Aug 12, 2021
Journal Journal of Microbiological Methods
Print ISSN 0167-7012
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 177
Article Number 106025
DOI https://doi.org/10.1016/j.mimet.2020.106025
Keywords Pseudomonas; Conjugation; TnSeq
Public URL https://uwe-repository.worktribe.com/output/6508170
Publisher URL https://doi.org/10.1016/j.mimet.2020.106025
Additional Information Supporting data: http://researchdata.uwe.ac.uk/583/

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