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Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola

O'Leary, Brendan M.; Neale, Helen C.; Geilfus, Christoph-Martin; Jackson, Robert W.; Arnold, Dawn L.; Preston, Gail M.

Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola Thumbnail


Authors

Brendan M. O'Leary

Helen Neale Helen2.Neale@uwe.ac.uk
Occasional Associate Lecturer - CHSS - DAS

Christoph-Martin Geilfus

Robert W. Jackson

Dawn Arnold Dawn.Arnold@uwe.ac.uk
Professor in Molecular Plant Pathology

Gail M. Preston



Abstract

© 2016 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. The apoplast is the arena in which endophytic pathogens such as Pseudomonas syringae grow and interact with plant cells. Using metabolomic and ion analysis techniques, this study shows how the composition of Phaseolus vulgaris leaf apoplastic fluid changes during the first six hours of compatible and incompatible interactions with two strains of P. syringae pv. phaseolicola (Pph) that differ in the presence of the genomic island PPHGI-1. Leaf inoculation with the avirulent island-carrying strain Pph 1302A elicited effector-triggered immunity (ETI) and resulted in specific changes in apoplast composition, including increases in conductivity, pH, citrate, γ-aminobutyrate (GABA) and K+, that are linked to the onset of plant defence responses. Other apoplastic changes, including increases in Ca2+, Fe2/3+ Mg2+, sucrose, β-cyanoalanine and several amino acids, occurred to a relatively similar extent in interactions with both Pph 1302A and the virulent, island-less strain Pph RJ3. Metabolic footprinting experiments established that Pph preferentially metabolizes malate, glucose and glutamate, but excludes certain other abundant apoplastic metabolites, including citrate and GABA, until preferred metabolites are depleted. These results demonstrate that Pph is well-adapted to the leaf apoplast metabolic environment and that loss of PPHGI-1 enables Pph to avoid changes in apoplast composition linked to plant defences.

Citation

O'Leary, B. M., Neale, H. C., Geilfus, C., Jackson, R. W., Arnold, D. L., & Preston, G. M. (2016). Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola. Plant, Cell and Environment, 39(10), 2172-2184. https://doi.org/10.1111/pce.12770

Journal Article Type Article
Acceptance Date May 23, 2016
Online Publication Date Jul 25, 2016
Publication Date Oct 1, 2016
Deposit Date Jun 9, 2016
Publicly Available Date Mar 29, 2024
Journal Plant Cell and Environment
Print ISSN 0140-7791
Electronic ISSN 1365-3040
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 39
Issue 10
Pages 2172-2184
DOI https://doi.org/10.1111/pce.12770
Keywords Pseudomonas syringae, leaf apoplast, apoplastic washing fluid, citrate, GABA, Phaseolus vulgaris, 17 metabolomics, metabolic footprinting, plant-microbe interactions, plant defence response
Public URL https://uwe-repository.worktribe.com/output/907651
Publisher URL http://dx.doi.org/10.1111/pce.12770
Additional Information Additional Information : This is the peer reviewed version of the following article: O'Leary, B., Neale, H., Geilfus, C.-M., Jackson, R. W., Arnold, D. L. and Preston, G. (2016) Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae pv. phaseolicola. Plant, Cell & Environment. ISSN 0140-7791, which has been published in final form at http://dx.doi.org/10.1111/pce.12770. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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