An improved conjugation method for Pseudomonas syringae

Neale, Helen C.; Hulin, Michelle T.; Harrison, Richard J.; Jackson, Robert W.; Mansfield, John W.; Arnold, Dawn L.

Abstract

In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.

• Journal Article Type: Article
• Acceptance Date: Aug 5, 2020
• Online Publication Date: Aug 11, 2020
• Publication Date: Oct 1, 2020
• Deposit Date: Aug 26, 2020
• Publicly Available Date: Aug 12, 2021
• Journal: Journal of Microbiological Methods
• Print ISSN: 0167-7012
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 177
• Article Number: 106025
• DOI: https://doi.org/10.1016/j.mimet.2020.106025
• Keywords: Pseudomonas; Conjugation; TnSeq
• Public URL: https://uwe-repository.worktribe.com/output/6508170
• Publisher URL: https://doi.org/10.1016/j.mimet.2020.106025
• Additional Information: Supporting data: http://researchdata.uwe.ac.uk/583/