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An electrochemical screen-printed sensor based on gold-nanoparticle-decorated reduced graphene oxide–carbon nanotubes composites for the determination of 17-β estradiol

Musa, Auwal; Kiely, Janice; Luxton, Richard; Honeychurch, Kevin C.

An electrochemical screen-printed sensor based on gold-nanoparticle-decorated reduced graphene oxide–carbon nanotubes composites for the determination of 17-β estradiol Thumbnail


Authors

Auwal Musa

Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor



Abstract

In this study, a screen-printed electrode (SPE) modified with gold-nanoparticle-decorated reduced graphene oxide–carbon nanotubes (rGO-AuNPs/CNT/SPE) was used for the determination of estradiol (E2). The AuNPs were produced through an eco-friendly method utilising plant extract, eliminating the need for severe chemicals, and remove the requirements of sophisticated fabrication methods and tedious procedures. In addition, rGO-AuNP serves as a dispersant for the CNT to improve the dispersion stability of CNTs. The composite material, rGO-AuNPs/CNT, underwent characterisation through scanning electron microscopy (SEM), ultraviolet–visible absorption spectroscopy (UV–vis), Fourier-transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM). The electrochemical performance of the modified SPE for estradiol oxidation was characterised using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The rGO-AuNPs/CNT/SPE exhibited a notable improvement compared to bare/SPE and GO-CNT/SPE, as evidenced by the relative peak currents. Additionally, we employed a baseline correction algorithm to accurately adjust the sensor response while eliminating extraneous background components that are typically present in voltammetric experiments. The optimised estradiol sensor offers linear sensitivity from 0.05–1.00 µM, with a detection limit of 3 nM based on three times the standard deviation (3δ). Notably, this sensing approach yields stable, repeatable, and reproducible outcomes. Assessment of drinking water samples indicated an average recovery rate of 97.5% for samples enriched with E2 at concentrations as low as 0.5 µM%, accompanied by only a modest coefficient of variation (%CV) value of 2.7%.

Citation

Musa, A., Kiely, J., Luxton, R., & Honeychurch, K. C. (2023). An electrochemical screen-printed sensor based on gold-nanoparticle-decorated reduced graphene oxide–carbon nanotubes composites for the determination of 17-β estradiol. Biosensors, 13(4), Article 491. https://doi.org/10.3390/bios13040491

Journal Article Type Article
Acceptance Date Apr 10, 2023
Online Publication Date Apr 19, 2023
Publication Date Apr 19, 2023
Deposit Date Apr 19, 2023
Publicly Available Date Apr 20, 2023
Journal Biosensors
Electronic ISSN 2079-6374
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 13
Issue 4
Article Number 491
Series Title This article belongs to the Special Issue Applications of Advanced Electrochemical (Bio)sensors in Environment, Food, and Medicine
DOI https://doi.org/10.3390/bios13040491
Keywords Estradiol; gold nanoparticles; graphene oxide; carbon nanotubes; disposable screen-printed electrodes
Public URL https://uwe-repository.worktribe.com/output/10635348
Publisher URL https://www.mdpi.com/2079-6374/13/4/491

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