Effects of chlorhexidine on a tongue-flora microcosm and VSC production using an in vitro biofilm perfusion model

Greenman, John; McKenzie, C.; Saad, S.; Wiegand, B.; Zguris, J. C.

doi:10.1088/1752-7155/2/4/046005

Urine utilisation by microbial fuel cells; Energy fuel for the future (2012)
Journal Article
Ieropoulos, I., Greenman, J., & Melhuish, C. (2012). Urine utilisation by microbial fuel cells; Energy fuel for the future. Physical Chemistry Chemical Physics, 14(1), 94-98. https://doi.org/10.1039/c1cp23213d

This communication reports for the first time the direct utilisation of urine in MFCs for the production of electricity. Different conversion efficiencies were recorded, depending on the amount treated. Elements such as N, P, K can be locked into new... Read More about Urine utilisation by microbial fuel cells; Energy fuel for the future.

Microbial fuel cells for robotics: Energy autonomy through artificial symbiosis (2012)
Journal Article
Ieropoulos, I., Greenman, J., Melhuish, C., & Horsfield, I. (2012). Microbial fuel cells for robotics: Energy autonomy through artificial symbiosis. ChemSusChem, 5(6), 1020-1026. https://doi.org/10.1002/cssc.201200283

The development of the microbial fuel cell (MFC) technology has seen an enormous growth over the last hundred years since its inception by Potter in 1911. The technology has reached a level of maturity that it is now considered to be a field in its o... Read More about Microbial fuel cells for robotics: Energy autonomy through artificial symbiosis.

Investigating the effects of fluidic connection between microbial fuel cells (2011)
Journal Article
Winfield, J., Ieropoulos, I., Greenman, J., & Dennis, J. (2011). Investigating the effects of fluidic connection between microbial fuel cells. Bioprocess and Biosystems Engineering, 34(4), 477-484. https://doi.org/10.1007/s00449-010-0491-x

Microbial fuel cells (MFCs) can 'treat' wastewater but individually are thermodynamically restricted. Scale-up might, therefore, require a plurality of units operating in a stack which could introduce losses simply through fluidic connections. Experi... Read More about Investigating the effects of fluidic connection between microbial fuel cells.

The overshoot phenomenon as a function of internal resistance in microbial fuel cells (2011)
Journal Article
Winfield, J., Ieropoulos, I., Greenman, J., & Dennis, J. (2011). The overshoot phenomenon as a function of internal resistance in microbial fuel cells. Bioelectrochemistry, 81(1), 22-27. https://doi.org/10.1016/j.bioelechem.2011.01.001

A method for assessing the performance of microbial fuel cells (MFCs) is the polarisation sweep where different external resistances are applied at set intervals (sample rates). The resulting power curves often exhibit an overshoot where both power a... Read More about The overshoot phenomenon as a function of internal resistance in microbial fuel cells.

Comparative effects of various commercially available mouthrinse formulations on oral malodour (2011)
Journal Article
Saad, S., Greenman, J., & Shaw, H. (2011). Comparative effects of various commercially available mouthrinse formulations on oral malodour. Oral Diseases, 17(2), 180-186. https://doi.org/10.1111/j.1601-0825.2010.01714.x

Objectives: The primary aim of this study was to compare a new mouthwash (SB12®) containing 0.025% chlorhexidine and 0.3% zinc for oral malodour reduction against four commercially available mouthwashes and negative control. A secondary aim was to co... Read More about Comparative effects of various commercially available mouthrinse formulations on oral malodour.

Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro (2011)
Journal Article
Thorn, R., Reynolds, D. M., & Greenman, J. (2011). Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro. Journal of Microbiological Methods, 84(2), 258-264. https://doi.org/10.1016/j.mimet.2010.12.001

Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro... Read More about Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro.

Small scale microbial fuel cells and different ways of reporting output (2010)
Journal Article
Ieropoulos, I., Winfield, J., Greenman, J., & Melhuish, C. (2010). Small scale microbial fuel cells and different ways of reporting output. ECS Transactions, 28(9), 1-9. https://doi.org/10.1149/1.3492221

The present study, reports on the findings of connecting 2 stacks of 48 MFCs and the importance of maturity and acclimation for the anodic biofilms. Furthermore, an attempt is made to emphasize the importance of a universal unit for quantifying power... Read More about Small scale microbial fuel cells and different ways of reporting output.

MFCs and algae (2010)
Journal Article
Ieropoulos, I. A., Greenman, J., & Sauer, M. (2010). MFCs and algae. ECS Transactions, 28(9), 23-30. https://doi.org/10.1149/1.3492223

Algae and photosynthetic bacteria may be integrated or associated with Microbial Fuel Cells MFCs in a number of different ways including: (1) use of whole (intact) or lipid-extracted lyzed cells as the primary carbon-energy source for anodic microbia... Read More about MFCs and algae.

The overshoot phenomenon as a function of internal resistance in microbial fuel cells (2010)
Journal Article
Winfield, J., Ieropoulos, I., & Greenman, J. (2010). The overshoot phenomenon as a function of internal resistance in microbial fuel cells. Journal of Biotechnology, 150(Supp), 23. https://doi.org/10.1016/j.jbiotec.2010.08.072

Effects of flow-rate, inoculum and time on the internal resistance of microbial fuel cells (2010)
Journal Article
Ieropoulos, I., Winfield, J., & Greenman, J. (2010). Effects of flow-rate, inoculum and time on the internal resistance of microbial fuel cells. Bioresource Technology, 101(10), 3520-3525. https://doi.org/10.1016/j.biortech.2009.12.108

To process large volumes of wastewater, microbial fuel cells (MFCs) would require anodophilic bacteria preferably operating at high flow-rates. The effect of flow-rate on different microbial consortia was examined during anodic biofilm development, u... Read More about Effects of flow-rate, inoculum and time on the internal resistance of microbial fuel cells.

Modelling the effects of pH on tongue biofilm using a sorbarod biofilm perfusion system (2010)
Journal Article
Taylor, B., & Greenman, J. (2010). Modelling the effects of pH on tongue biofilm using a sorbarod biofilm perfusion system. Journal of Breath Research, 4(1), 017107. https://doi.org/10.1088/1752-7155/4/1/017107

The pH of the tongue biofilm is likely to influence microbial composition and ecology with consequent effects on the metabolic activities and generation of volatile sulfur compounds (VSC) and other malodour gasses. The aim of this study was to identi... Read More about Modelling the effects of pH on tongue biofilm using a sorbarod biofilm perfusion system.

Improved energy output levels from small-scale Microbial Fuel Cells (2010)
Journal Article
Ieropoulos, I., Greenman, J., & Melhuish, C. (2010). Improved energy output levels from small-scale Microbial Fuel Cells. Bioelectrochemistry, 78(1), 44-50. https://doi.org/10.1016/j.bioelechem.2009.05.009

This study reports on the findings from the investigation into small-scale (6.25mL) MFCs, connected together as a network of multiple units. The MFCs contained unmodified (no catalyst) carbon fibre electrodes and for initial and later experiments, a... Read More about Improved energy output levels from small-scale Microbial Fuel Cells.

Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores (2010)
Journal Article
Salisbury, V. C., Robinson, G. M., Lee, S. W., Greenman, J., & Reynolds, D. M. (2010). Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores. Letters in Applied Microbiology, 50(3), 289-294. https://doi.org/10.1111/j.1472-765X.2009.02790.x

Aims: Electrochemically activated solutions (ECAS) are generated from halide salt solutions via specially designed electrolytic cells. The active solutions are known to possess high biocidal activity against a wide range of target microbial species,... Read More about Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores.

Comparative analysis of different polymer materials for the construction of microbial fuel cell stacks (2010)
Journal Article
Ledezma, P., Ieropoulos, I., & Greenman, J. (2010). Comparative analysis of different polymer materials for the construction of microbial fuel cell stacks. Journal of Biotechnology, 150, 143-143. https://doi.org/10.1016/j.jbiotec.2010.08.373

A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments (2009)
Journal Article
Thorn, R., & Greenman, J. (2009). A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments. Journal of Applied Microbiology, 107(6), 2070-2079. https://doi.org/10.1111/j.1365-2672.2009.04398.x

Aims: To develop an in vitro flat-bed perfusion biofilm model that could be used to determine the antimicrobial efficacy of topically applied treatments. Methods and Results: Pseudomonas aeruginosa and Staphylococcus aureus biofilms were grown within... Read More about A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments.

Landfill leachate treatment with microbial fuel cells; scale-up through plurality (2009)
Journal Article
Gálvez, A., Greenman, J., & Ieropoulos, I. (2009). Landfill leachate treatment with microbial fuel cells; scale-up through plurality. Bioresource Technology, 100(21), 5085-5091. https://doi.org/10.1016/j.biortech.2009.05.061

Three Microbial Fuel Cells (MFCs) were fluidically connected in series, with a single feed-line going into the 1st column through the 2nd column and finally as a single outflow coming from the 3rd column. Provision was also made for re-circulation in... Read More about Landfill leachate treatment with microbial fuel cells; scale-up through plurality.

Electricity from landfill leachate using microbial fuel cells: Comparison with a biological aerated filter (2009)
Journal Article
Gálvez, A., Greenman, J., Giusti, L., & Ieropoulos, I. (2009). Electricity from landfill leachate using microbial fuel cells: Comparison with a biological aerated filter. Enzyme and Microbial Technology, 44(2), 112-119. https://doi.org/10.1016/j.enzmictec.2008.09.012

Four experimental columns were employed in this study to investigate their performance under wastewater treatment conditions. One column was set-up as a biological aerated filter and the remaining three were set-up as microbial fuel cells (MFCs), two... Read More about Electricity from landfill leachate using microbial fuel cells: Comparison with a biological aerated filter.

A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments (2009)
Journal Article
Thorn, R., & Greenman, J. (2009). A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments. Journal of Applied Microbiology, 107(6), 2070-2079. https://doi.org/10.1111/j.1365-2672.2009.04398.x

To develop an in vitro flat-bed perfusion biofilm model that could be used to determine the antimicrobial efficacy of topically applied treatments. METHODS AND RESULTS: Pseudomonas aeruginosa and Staphylococcus aureus biofilms were grown within co... Read More about A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments.

Effects of chlorhexidine on a tongue-flora microcosm and VSC production using an in vitro biofilm perfusion model (2008)
Journal Article
Greenman, J., McKenzie, C., Saad, S., Wiegand, B., & Zguris, J. C. (2008). Effects of chlorhexidine on a tongue-flora microcosm and VSC production using an in vitro biofilm perfusion model. Journal of Breath Research, 2(4), 046005. https://doi.org/10.1088/1752-7155/2/4/046005

An in vitro perfusion biofilm model, derived from tongue-scrape microflora removed from one individual, was employed to study sulfide biogenesis and the effects of repeated exposure to chlorhexidine (CHX). Volatile sulfur compounds (VSC) were measure... Read More about Effects of chlorhexidine on a tongue-flora microcosm and VSC production using an in vitro biofilm perfusion model.

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