Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Atanassov, Plamen; Bestetti, Massimiliano; Cristiani, Pierangela; Trasatti, Stefano Pm; Guerrini, Edoardo; Babanova, Sofia; Grattieri, Matteo; Santoro, Carlo

Abstract

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

Citation

Atanassov, P., Bestetti, M., Cristiani, P., Trasatti, S. P., Guerrini, E., Babanova, S., …Santoro, C. (2015). Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. Electroanalysis, 27(2), 327-335. https://doi.org/10.1002/elan.201400543

• Journal Article Type: Article
• Acceptance Date: Jan 22, 2015
• Publication Date: Jan 1, 2015
• Deposit Date: May 1, 2018
• Journal: Electroanalysis
• Print ISSN: 1040-0397
• Electronic ISSN: 1521-4109
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 2
• Pages: 327-335
• DOI: https://doi.org/10.1002/elan.201400543
• Keywords: biosensor, bilirubin oxidase, oxygen measurement, biofilm, biocathode, MFC
• Public URL: https://uwe-repository.worktribe.com/output/839329
• Publisher URL: http://dx.doi.org/10.1002/elan.201400543