Xiao Long Yang
Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase
Yang, Xiao Long; Friedrich, Steffen; Yin, Sen; Piech, Oliver; Williams, Katherine; Simpson, Thomas J; Cox, Russell
Authors
Steffen Friedrich
Sen Yin
Oliver Piech
Katherine Williams
Thomas J Simpson
Russell Cox
Abstract
Exchange of 32 different sub-fragments of the C-methyltransferase (C-MeT), pseudo-ketoreductase (ΨKR) and ketoreductase (KR) catalytic domains of the tenellin iterative Type I polyketide synthase non ribosomal peptide synthetase (PKS-NRPS) TENS by homologous fragments from the desmethylbassianin (DMBS) and militarinone (MILS) PKS-NRPS led to the creation of chimeric synthetases in which programming fidelity was altered, resulting in the production of mixtures of products with different methylation patterns and chain lengths. Swap of KR domain subfragments with the homologous fragments from the KR of the heptaketide militarinone synthetase resulted in the synthesis of penta, hexa and heptaketides. The results of these and previous experiments are rationalised by considering the existence of competition for acyl-carrier protein (ACP) bound substrates between different catalytic domains of the PKS. In particular, competition between the C-MeT and ketoreductase domains (KR) can account for methylation programming, and competition between the KR and the off-loading NRPS accounts for chain-length selectivity.
Citation
Yang, X. L., Friedrich, S., Yin, S., Piech, O., Williams, K., Simpson, T. J., & Cox, R. (2019). Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase. Chemical Science, 10(36), 8478-8489. https://doi.org/10.1039/C9SC03173A
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 28, 2019 |
Online Publication Date | Jul 30, 2019 |
Publication Date | Jul 30, 2019 |
Deposit Date | Apr 10, 2024 |
Publicly Available Date | Apr 10, 2024 |
Journal | Chemical Science |
Print ISSN | 2041-6520 |
Electronic ISSN | 2041-6539 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 36 |
Pages | 8478-8489 |
DOI | https://doi.org/10.1039/C9SC03173A |
Public URL | https://uwe-repository.worktribe.com/output/11881281 |
Publisher URL | http://dx.doi.org/10.1039/C9SC03173A |
Files
Molecular basis of methylation and chain-length programming in a fungal iterative highly reducing polyketide synthase
(2.3 Mb)
PDF
Licence
http://creativecommons.org/licenses/by-nc/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by-nc/4.0/
You might also like
In silico analyses of maleidride biosynthetic gene clusters
(2022)
Journal Article
The use of yellow fluorescent hybrids to indicate mating in Trypanosoma brucei
(2008)
Journal Article
Downloadable Citations
About UWE Bristol Research Repository
Administrator e-mail: repository@uwe.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search