Radhika Desikan
Tools to investigate reaction oxygen species-sensitive signaling proteins
Desikan, Radhika; Neill, Steven; Slinn, Jenna; Hancock, John T.
Authors
Steven Neill Steven.Neill@uwe.ac.uk
Jenna Slinn
John Hancock John.Hancock@uwe.ac.uk
Professor in Cell Signalling
Contributors
John Hancock John.Hancock@uwe.ac.uk
Editor
Abstract
The thiols groups of cysteine residues on proteins are attractive oxidative targets for modification by reactive oxygen species, such as hydrogen peroxide (H2O2). Such modification can lead to important cellular signaling processes that ultimately result in modification of physiology of the organism. To identify such proteins that are amenable to oxidative modification, different methods can be used. Here, two such approaches are described: one being the use of fluorescent thiol derivatives, and the second being the use of genetic mutants that are mutated in thiol residues. Using the model plant Arabidopsis thaliana, cell cultures, and whole plants, we describe these tools to help the reader understand the function of such thiol modification on plant responses.
Citation
Desikan, R., Neill, S., Slinn, J., & Hancock, J. T. (2009). Tools to investigate reaction oxygen species-sensitive signaling proteins. In J. T. Hancock (Ed.), Redox-Mediated Signal Transduction (84-96). Humana Press. https://doi.org/10.1007/978-1-59745-129-1_7
Publication Date | Jan 1, 2009 |
---|---|
Journal | Tools to Investigate Reaction Oxygen |
Print ISSN | 1064-3745 |
Peer Reviewed | Peer Reviewed |
Volume | 476 |
Pages | 84-96 |
Series Title | Methods in Molecular Biology |
Book Title | Redox-Mediated Signal Transduction |
ISBN | 978-1-58829-842-3 |
DOI | https://doi.org/10.1007/978-1-59745-129-1_7 |
Keywords | glyceraldehyde 3-phosphate dehydrogenase, histidine kinase, hydrogen peroxide, 5′-iodoacetamide fluorescein, reactive oxygen species, stomatal guard cells, thiol labeling |
Public URL | https://uwe-repository.worktribe.com/output/1001925 |
Publisher URL | http://dx.doi.org/10.1007/978-1-59745-129-1_7 |
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