Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma (2013)
Presentation / Conference
Robinson, G. M., Thorn, R., Hancock, C., & Reynolds, D. M. (2013, May). Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma. Paper presented at 3rd International Conference on Bio-Sensing Technology, Sitges, Spain

Introduction: Maintenance of a clean, uncontaminated healthcare environment is instrumental in the delivery of modern healthcare. Nonthermal plasma is a technology with potential applications for sterilisation and disinfection in biological and medi... Read More about Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma.

Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay (2013)
Journal Article
Reynolds, D. M., Thorn, R. M., Thorn, R., Reynolds, D. M., & Robinson, G. M. (2013). Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay. Antimicrobial Agents and Chemotherapy, 57(5), 2216-2225. https://doi.org/10.1128/AAC.02589-12

The main aim of this study was to develop a standardized experimental assay to enable differential antimicrobial comparisons of test biocidal aerosols. This study represents the first chlorine-matched comparative assessment of the antimicrobial activ... Read More about Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay.

Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor (2013)
Presentation / Conference
Robinson, G. M., Anderson, E., Salisbury, V., Mehta, P., Sandvold, M., & Reynolds, D. M. (2013, April). Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor. Poster presented at American Association for Cancer Research Annual Meeting, Washington D.C., USA

Cytarabine (ara-C) is an analogue of deoxycytidine with an established role in treating AML. Cytarabine depends on the human equilibrative nucleoside transporter-1 (hENT-1) for cellular entry (1). Loss of hENT-1 function in vitro has been shown to re... Read More about Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor.

The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions (2013)
Journal Article
Robinson, G., Thorn, R., & Reynolds, D. (2013). The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions. International Journal of Molecular Sciences, 14(1), 457-469. https://doi.org/10.3390/ijms14010457

Electrochemically activated solutions (ECAS) are generated by electrolysis of NaCl solutions, and demonstrate broad spectrum antimicrobial activity and high environmental compatibility. The biocidal efficacy of ECAS at the point of production is wide... Read More about The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions.

Characterization of the Salmonella bacteriophage vB_SenS-Ent1 (2012)
Journal Article
Reynolds, D., Nelson, S., Turner, D., Hezwani, M., Nelson, S., Salisbury, V., & Reynolds, D. M. (2012). Characterization of the Salmonella bacteriophage vB_SenS-Ent1. Journal of General Virology, 93(PART 9), 2046-2056. https://doi.org/10.1099/vir.0.043331-0

The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in d... Read More about Characterization of the Salmonella bacteriophage vB_SenS-Ent1.

Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments (2012)
Journal Article
Lee, S. W., Thorn, R., Robinson, G., Greenman, J., & Reynolds, D. M. (2012). Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments. European Journal of Clinical Microbiology and Infectious Diseases, 31(5), 641-653. https://doi.org/10.1007/s10096-011-1369-9

Due to the limitations associated with the use of existing biocidal agents, there is a need to explore new methods of disinfection to help maintain effective bioburden control, especially within the healthcare environment. The transformation of low m... Read More about Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments.

Constructing a coherent STEM strategy with schools (2012)
Journal Article
Hooper, A. P., Reynolds, D. M., & Lavelle, M. (2012). Constructing a coherent STEM strategy with schools

Most universities run STEM enrichment activities with local schools. At UWE we have run activities with respect to mathematics (Maths Challenge, Maths Event Day, FUNMaths Roadshows), Engineering (Engineering activity day, Bloodhound), Science (Scienc... Read More about Constructing a coherent STEM strategy with schools.

Application of bacterial bioluminescence to assess the efficacy of fast-acting biocides (2011)
Journal Article
Robinson, G., Tonks, K. M., Thorn, R., & Reynolds, D. M. (2011). Application of bacterial bioluminescence to assess the efficacy of fast-acting biocides. Antimicrobial Agents and Chemotherapy, 55(11), 5214-5219. https://doi.org/10.1128/AAC.00489-11

Traditional microbiological techniques are used to provide reliable data on the rate and extent of kill for a range of biocides. However, such techniques provide very limited data regarding the initial rate of kill of fast-acting biocides over very s... Read More about Application of bacterial bioluminescence to assess the efficacy of fast-acting biocides.

The use of bioluminescent bacterial spores as a rapid reporter of successful decontamination (2011)
Presentation / Conference
Robinson, G. M., & Reynolds, D. M. (2011, October). The use of bioluminescent bacterial spores as a rapid reporter of successful decontamination. Poster presented at 2nd International Conference on Bio-Sensing Technology, Amsterdam, Netherlands

The efficacy of aerosolised electrochemically activated solutions for rapid decontamination (2011)
Presentation / Conference
Robinson, G. M., Thorn, R., & Reynolds, D. M. (2011, September). The efficacy of aerosolised electrochemically activated solutions for rapid decontamination. Poster presented at Health Protection Agency Conference, Warwick, UK

Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro (2011)
Journal Article
Thorn, R., Reynolds, D. M., & Greenman, J. (2011). Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro. Journal of Microbiological Methods, 84(2), 258-264. https://doi.org/10.1016/j.mimet.2010.12.001

Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro... Read More about Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro.

Bacterial bioluminescence as a tool for monitoring the real-time activity of antimicrobial agents (2010)
Presentation / Conference
Robinson, G. M., Lee, S., Thorn, R., & Reynolds, D. M. (2010, November). Bacterial bioluminescence as a tool for monitoring the real-time activity of antimicrobial agents. Presented at International Conference on Antimicrobial Research, Valladolid, Spain

Continuous fluorescence excitation-emission matrix monitoring of river organic matter (2010)
Journal Article
Carstea, E. M., Baker, A., Bieroza, M., & Reynolds, D. M. (2010). Continuous fluorescence excitation-emission matrix monitoring of river organic matter. Water Research, 44(18), 5356-5366. https://doi.org/10.1016/j.watres.2010.06.036

Real-time fluorescence monitoring has been mostly performed in marine systems, with little progress being made in the application of fluorescence excitation-emission matrix (EEM) spectroscopy, especially for freshwater monitoring. This paper presents... Read More about Continuous fluorescence excitation-emission matrix monitoring of river organic matter.

Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores (2010)
Journal Article
Salisbury, V. C., Robinson, G. M., Lee, S. W., Greenman, J., & Reynolds, D. M. (2010). Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores. Letters in Applied Microbiology, 50(3), 289-294. https://doi.org/10.1111/j.1472-765X.2009.02790.x

Aims: Electrochemically activated solutions (ECAS) are generated from halide salt solutions via specially designed electrolytic cells. The active solutions are known to possess high biocidal activity against a wide range of target microbial species,... Read More about Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores.

Real-time monitoring of river water quality using in-line continuous acquisition of fluorescence excitation and emission matrices (2009)
Presentation / Conference
Reynolds, D. M. (2009, December). Real-time monitoring of river water quality using in-line continuous acquisition of fluorescence excitation and emission matrices. Poster presented at American Geophysical Union Fall Meeting, San Francisco, USA

Birmingham, UK. River water was diverted to a portable laboratory via a continuous flow pump and filter system. Fluorescence excitation-emission matrices data was recorded every 3 minutes using a flow cell (1cm pathlength) coupled to a fiber optic pr... Read More about Real-time monitoring of river water quality using in-line continuous acquisition of fluorescence excitation and emission matrices.

The effect of temperature on the fluorescence of DOM (2009)
Presentation / Conference
Reynolds, D. M., Baker, A., & Carstea, E. (2009, December). The effect of temperature on the fluorescence of DOM. Paper presented at American Geophysical Union Fall Meeting, San Francisco, USA

It is understood that all fluorescence analyses essentially observes the fluorescence of a given sample in a 'quenched state'. Even so, detailed literature concerning the effects of lower temperatures (below 4 degrees Celsius) is surprisingly sparse.... Read More about The effect of temperature on the fluorescence of DOM.

Monitoring the real-time in situ decontamination of surfaces and objects using bacterial bioluminescence (2009)
Presentation / Conference
Robinson, G. M., Lee, S., Greenman, J., Salisbury, V., & Reynolds, D. M. (2009, November). Monitoring the real-time in situ decontamination of surfaces and objects using bacterial bioluminescence. Poster presented at 1st International Conference on Bio-Sensing Technology, Bristol, UK

Bacterial bioluminescence as a real-time bio reporter of the efficacy of fast-acting disinfectants (2009)
Presentation / Conference
Robinson, G. M., Tonks, K., Lee, S., Greenman, J., Salisbury, V., & Reynolds, D. M. (2009, November). Bacterial bioluminescence as a real-time bio reporter of the efficacy of fast-acting disinfectants. Poster presented at 1st International Conference on Bio-Sensing Technology, Bristol, UK

Changes in freshwater organic matter fluorescence intensity with freezing/thawing and dehydration/rehydration (2009)
Journal Article
Hudson, N., Baker, A., Reynolds, D. M., Carliell-Marquet, C., & Ward, D. (2009). Changes in freshwater organic matter fluorescence intensity with freezing/thawing and dehydration/rehydration. Journal of Geophysical Research, 114(4), G00F08. https://doi.org/10.1029/2008JG000915

The effects of photodegradation and biodegradation upon aquatic organic matter lability have been extensively researched in all aquatic systems because of the impact of these. processes upon carbon cycling, with most studies undertaken on the dissolv... Read More about Changes in freshwater organic matter fluorescence intensity with freezing/thawing and dehydration/rehydration.

Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England (2008)
Journal Article
Hudson, N., Baker, A., Reynolds, D. M., Ward, D., Brunsdon, C., Carliell-Marquet, C., & Browning, S. (2008). Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England. Science of the Total Environment, 391(1), 149-158. https://doi.org/10.1016/j.scitotenv.2007.10.054

The fluorescence intensities of tryptophan-like, tyrosine-like and humic-like materials were determined using excitation-emission-matrices (EEMs) for a wide range of samples including natural surface waters, sewage and industrial effluents and waters... Read More about Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England.