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Characterization of the Salmonella bacteriophage vB_SenS-Ent1

Reynolds, Darren; Nelson, Shona; Turner, Dann; Nelson, S.M.; Hezwani, Mohammed; Reynolds, Darren M.; Salisbury, Vyv

Authors

Darren Reynolds

Shona Nelson

Dann Turner

Mohammed Hezwani mohammed2.hezwani@uwe.ac.uk

Vyv Salisbury vyv.salisbury@uwe.ac.uk



Abstract

The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37°C is 6.73 × 10 -9 ml min -1 . Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37°C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the Salmonella phages SETP3, SE2 and KS7 (SS3e) and the Escherichia phages K1G, K1H, K1ind1 and K1ind3. © 2012 SGM.

Citation

Reynolds, D., Nelson, S., Turner, D., Hezwani, M., Nelson, S., Salisbury, V., & Reynolds, D. M. (2012). Characterization of the Salmonella bacteriophage vB_SenS-Ent1. Journal of General Virology, 93(PART 9), 2046-2056. https://doi.org/10.1099/vir.0.043331-0

Journal Article Type Article
Publication Date Sep 1, 2012
Journal Journal of General Virology
Print ISSN 0022-1317
Electronic ISSN 1465-2099
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 93
Issue PART 9
Pages 2046-2056
DOI https://doi.org/10.1099/vir.0.043331-0
Keywords salmonella bacteriophage
Public URL https://uwe-repository.worktribe.com/output/956103
Publisher URL http://dx.doi.org/10.1099/vir.0.043331-0