Effectiveness of the human oral microbe identification microarray in identifying periodontal pathogens: A Systematic review (2022)
Journal Article
Udoh, S., Adukwu, E., Varadi, A., & Saad, S. (2022). Effectiveness of the human oral microbe identification microarray in identifying periodontal pathogens: A Systematic review. Applied Microbiology, 2(3), 614-625. https://doi.org/10.3390/applmicrobiol2030047

The aim of this review was to investigate the effectiveness of the Human Oral Microbe Identification Microarray (HOMIM) in identifying and quantifying bacterial species of the oral microbiome in periodontal disease. The search for articles was conduc... Read More about Effectiveness of the human oral microbe identification microarray in identifying periodontal pathogens: A Systematic review.

Variants of the EAAT2 Glutamate Transporter Gene Promoter Are Associated with Cerebral Palsy in Preterm Infants (2017)
Journal Article
Rajatileka, S., Odd, D., Robinson, M. T., Spittle, A. C., Dwomoh, L., Williams, M., …Varadi, A. (2018). Variants of the EAAT2 Glutamate Transporter Gene Promoter Are Associated with Cerebral Palsy in Preterm Infants. Molecular Neurobiology, 55(3), 2013-2024. https://doi.org/10.1007/s12035-017-0462-1

© 2017, The Author(s). Preterm delivery is associated with neurodevelopmental impairment caused by environmental and genetic factors. Dysfunction of the excitatory amino acid transporter 2 (EAAT2) and the resultant impaired glutamate uptake can lead... Read More about Variants of the EAAT2 Glutamate Transporter Gene Promoter Are Associated with Cerebral Palsy in Preterm Infants.

Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR) (2016)
Journal Article
Turner, A., Sasse, J., & Varadi, A. (2016). Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR). BMC Medical Genetics, 17, Article 75. https://doi.org/10.1186/s12881-016-0334-y

© 2016 The Author(s). Objectives: Inherited disorders of haemoglobin are the world's most common genetic diseases, resulting in significant morbidity and mortality. The large number of mutations associated with the haemoglobin beta gene (HBB) makes g... Read More about Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR).

Association between neonatal resuscitation and a single nucleotide polymorphism rs1835740 (2016)
Journal Article
Odd, D., Varadi, A., Rajatileka, S., Molnár, E., & Luyt, K. (2016). Association between neonatal resuscitation and a single nucleotide polymorphism rs1835740. Acta Paediatrica, 105(7), e307-e312. https://doi.org/10.1111/apa.13421

© 2016 Foundation Acta Pædiatrica. Published by John Wiley & Sons Ltd. Aim The aim of this work was to test whether three single nucleotide polymorphisms (SNPs) implicated in glutamate homoeostasis or signalling and cellular survival are associated... Read More about Association between neonatal resuscitation and a single nucleotide polymorphism rs1835740.

Isolation of human genomic DNA for genetic analysis from premature neonates: A comparison between newborn dried blood spots, whole blood and umbilical cord tissue (2013)
Journal Article
Váradi, A., Molnár, E., Rajatileka, S., Luyt, K., El-Bokle, M., Williams, M., …Varadi, A. (2013). Isolation of human genomic DNA for genetic analysis from premature neonates: A comparison between newborn dried blood spots, whole blood and umbilical cord tissue. BMC Genetics, 14(105), https://doi.org/10.1186/1471-2156-14-105

Background: Genotyping requires biological sample collection that must be reliable, convenient and acceptable for patients and clinicians. Finding the most optimal procedure of sample collection for premature neonates who have a very limited blood vo... Read More about Isolation of human genomic DNA for genetic analysis from premature neonates: A comparison between newborn dried blood spots, whole blood and umbilical cord tissue.

MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway (2012)
Journal Article
Brozzi, F., Lajus, S., Diraison, F., Rajatileka, S., Hayward, K., Regazzi, R., …Varadi, A. (2012). MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway. Molecular Biology of the Cell, 23(22), 4444-4455. https://doi.org/10.1091/mbc.E12-05-0369

Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA)–anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin V... Read More about MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.

Molecular mechanism of Myosin Va recruitment to dense core secretory granules (2012)
Journal Article
Brozzi, F., Diraison, F., Lajus, S., Rajatileka, S., Philips, T., Regazzi, R., …Varadi, A. (2012). Molecular mechanism of Myosin Va recruitment to dense core secretory granules. Traffic, 13(1), 54-69. https://doi.org/10.1111/j.1600-0854.2011.01301.x

The brain-spliced isoform of Myosin Va (BR-MyoVa) plays an important role in the transport of dense core secretory granules (SGs) to the plasma membrane in hormone and neuropeptide-producing cells. The molecular composition of the protein complex tha... Read More about Molecular mechanism of Myosin Va recruitment to dense core secretory granules.

Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells (2011)
Journal Article
Avent, N. D., Bommer, U. A., Diraison, F., Hayward, K., Sanders, K. L., Brozzi, F., …Varadi, A. (2011). Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells. Diabetologia, 54(2), 368-379. https://doi.org/10.1007/s00125-010-1958-7

Aims/hypothesis: This study used proteomics and biochemical approaches to identify novel glucose-regulated proteins and to unveil their role in pancreatic beta cell function. Translationally controlled tumour protein (TCTP) was identified to be one s... Read More about Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells.

Myosin Va transports dense core secretory vesicles in pancreatic MIN6 β-cells (2005)
Journal Article
Varadi, A., Tsuboi, T., & Rutter, G. A. (2005). Myosin Va transports dense core secretory vesicles in pancreatic MIN6 β-cells. Molecular Biology of the Cell, 16(6), 2670-2680. https://doi.org/10.1091/mbc.E04-11-1001

The role of unconventional myosins in neuroendocrine cells is not fully understood, with involvement suggested in the movement of both secretory vesicles and mitochondria. Here, we demonstrate colocalization of myosin Va (MyoVa) with insulin in pancr... Read More about Myosin Va transports dense core secretory vesicles in pancreatic MIN6 β-cells.

Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1 (2004)
Journal Article
Varadi, A., Johnson-Cadwell, L. I., Cirulli, V., Yoon, Y., Allan, V. J., & Rutter, G. A. (2004). Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1. Journal of Cell Science, 117(19), 4389-4400. https://doi.org/10.1242/jcs.01299

While the subcellular organisation of mitochondria is likely to influence many aspects of cell physiology, its molecular control is poorly understood. Here, we have investigated the role of the retrograde motor protein complex, dynein-dynactin, in mi... Read More about Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1.

Involvement of conventional kinesin in glucose-stimulate secretory granule movements and exocytosis in clonal pancreatic β-cells (2002)
Journal Article
Varadi, A., Ainscow, E., Allan, V. J., & Rutter, G. A. (2002). Involvement of conventional kinesin in glucose-stimulate secretory granule movements and exocytosis in clonal pancreatic β-cells. Journal of Cell Science, 115(21), 4177-4189. https://doi.org/10.1242/jcs.00083

Recruitment of secretory vesicles to the cell surface is essential for the sustained secretion of insulin in response to glucose. At present, the molecular motors involved in this movement, and the mechanisms whereby they may be regulated, are undefi... Read More about Involvement of conventional kinesin in glucose-stimulate secretory granule movements and exocytosis in clonal pancreatic β-cells.

Dynamic imaging of endoplasmic reticulum Ca2+ concentration in insulin-secreting MIN6 cells using recombinant targeted cameleons: Roles of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)-2 and ryanodine receptors (2002)
Journal Article
Varadi, A., & Rutter, G. A. (2002). Dynamic imaging of endoplasmic reticulum Ca2+ concentration in insulin-secreting MIN6 cells using recombinant targeted cameleons: Roles of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)-2 and ryanodine receptors. Diabetes, 51(S1), 190-201. https://doi.org/10.2337/diabetes.51.2007.S190

The endoplasmic reticulum (ER) plays a pivotal role in the regulation of cytosolic Ca2+ concentrations ([Ca2+]cyt) and hence in insulin secretion from pancreatic β-cells. However, the molecular mechanisms involved in both the uptake and release of Ca... Read More about Dynamic imaging of endoplasmic reticulum Ca2+ concentration in insulin-secreting MIN6 cells using recombinant targeted cameleons: Roles of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)-2 and ryanodine receptors.