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David McCalley

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David McCalley

Professor in Bio-Analytical Science


Managing the column equilibration time in hydrophilic interaction chromatography (2019)
Journal Article
McCalley, D. V. (2020). Managing the column equilibration time in hydrophilic interaction chromatography. Journal of Chromatography A, 1612, https://doi.org/10.1016/j.chroma.2019.460655

For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial equilibration was demonstrated in gradient elution after purging with as little as 12 column volumes of mobile phase. Relative standard deviations of... Read More about Managing the column equilibration time in hydrophilic interaction chromatography.

Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phase (2019)
Journal Article
McCalley, D. V., & Guillarme, D. (2020). Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phase. Journal of Chromatography A, 1610, https://doi.org/10.1016/j.chroma.2019.460562

© 2019 Elsevier B.V. A wide pore (1000 Å) diphenyl stationary phase was evaluated for the analysis of monoclonal antibodies (mAbs), comparing a conventional mobile phase of acetonitrile-water containing overall 0.1% trifluoracetic acid (TFA) with a s... Read More about Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phase.

Hydrophilic interaction liquid chromatography: An update (2019)
Journal Article
McCalley, D. V. (2019). Hydrophilic interaction liquid chromatography: An update. Lc Gc Europe, 32(3), 114-125

This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantage... Read More about Hydrophilic interaction liquid chromatography: An update.

Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration (2018)
Journal Article
Heaton, J. C., Smith, N. W., & McCalley, D. V. (2019). Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration. Analytica Chimica Acta, 1045, 141-151. https://doi.org/10.1016/j.aca.2018.08.051

© 2018 Elsevier B.V. The separation of some zwitterionic, basic and neutral antibiotic and antiretroviral compounds was studied using hydrophilic interaction chromatography (HILIC) on bare silica, bonded amide and urea superficially porous phases. T... Read More about Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration.

A study of column equilibration time in hydrophilic interaction chromatography (2018)
Journal Article
McCalley, D. V. (2018). A study of column equilibration time in hydrophilic interaction chromatography. Journal of Chromatography A, 1554, 61-70. https://doi.org/10.1016/j.chroma.2018.04.016

© 2018 Elsevier B.V. The time taken to achieve full column equilibration for isocratic analysis of acidic, basic, and neutral solutes in hydrophilic interaction liquid chromatography (HILIC) was compared using the mobile phase disturbance method, col... Read More about A study of column equilibration time in hydrophilic interaction chromatography.

A study of the analysis of acidic solutes by hydrophilic interaction chromatography (2017)
Journal Article
McCalley, D. V. (2018). A study of the analysis of acidic solutes by hydrophilic interaction chromatography. Journal of Chromatography A, 1534, 64-74. https://doi.org/10.1016/j.chroma.2017.12.045

© 2017 Elsevier B.V. The analysis of acidic solutes was compared on a cross-linked bonded amino phase and a neutral hybrid inorganic-organic amide phase, previously shown to give reasonable retention of acidic solutes. The amino column gave strong se... Read More about A study of the analysis of acidic solutes by hydrophilic interaction chromatography.

Understanding and manipulating the separation in hydrophilic interaction liquid chromatography (2017)
Journal Article
McCalley, D. V. (2017). Understanding and manipulating the separation in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1523, 49-71. https://doi.org/10.1016/j.chroma.2017.06.026

© 2017 Elsevier B.V. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a valuable complimentary technique to reversed-phase (RP), being especially suited for the analysis of polar and ionised solutes, which are difficult to retain... Read More about Understanding and manipulating the separation in hydrophilic interaction liquid chromatography.

Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography (2016)
Journal Article
McCalley, D. V. (2017). Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1483, 71-79. https://doi.org/10.1016/j.chroma.2016.12.035

© 2016 Elsevier B.V. Trifluoracetic acid (TFA) added to the aqueous acetonitrile mobile phase induces some unexpected changes in the ionic component of retention in hydrophilic interaction separations when using Type B silica and amide-bonded silica... Read More about Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography.

Recent developments in support materials for use in high performance liquid chromatography (2016)
Journal Article
McCalley, D. V. (2016). Recent developments in support materials for use in high performance liquid chromatography

A number of advances have taken place in the last decade in the area of support materials for high performance liquid chromatography (HPLC). These developments have included the use of very small porous particles (< 2.0 micron diameter), new types of... Read More about Recent developments in support materials for use in high performance liquid chromatography.

Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure (2016)
Journal Article
Yui, Y., Miyazaki, S., Ma, Y., Ohira, M., Fiehn, O., Ikegami, T., …Tanaka, N. (2016). Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure. Journal of Chromatography A, 1450, 45-52. https://doi.org/10.1016/j.chroma.2016.04.076

© 2016 Elsevier B.V. Separation of diastereomers of DL-α-tocopherol was studied by reversed-phase liquid chromatography using three types of stationary phases, polymeric ODS, polymeric C30, and monomeric ODS. Polymeric ODS stationary phase (Inertsil... Read More about Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure.

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