Skip to main content

Research Repository

Advanced Search

Understanding and manipulating the separation in hydrophilic interaction liquid chromatography

McCalley, David V.



© 2017 Elsevier B.V. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a valuable complimentary technique to reversed-phase (RP), being especially suited for the analysis of polar and ionised solutes, which are difficult to retain in RP. For solutes amenable to both separation mechanisms, HILIC provides a different selectivity to RP, and also offers possibilities as an orthogonal mechanism for 2-dimensional LC when combined with RP. HILIC has further advantages of lower column back pressures, and increased sensitivity with mobile phase evaporative detectors such as electrospray mass spectrometry. This review covers progress in our understanding of the HILIC technique, principally over the last ten years, including the classification of columns, the factors that control retention and selectivity, and attempts to model the separation process and its kinetics.


McCalley, D. V. (2017). Understanding and manipulating the separation in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1523, 49-71.

Journal Article Type Review
Acceptance Date Jun 11, 2017
Online Publication Date Jun 13, 2017
Publication Date Nov 10, 2017
Journal Journal of Chromatography A
Print ISSN 0021-9673
Electronic ISSN 1873-3778
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1523
Pages 49-71
Keywords hydrophilic interaction chromatography, HILIC, retention, selectivity, additives
Public URL
Publisher URL


You might also like

Downloadable Citations