Hydrophilic interaction liquid chromatography: An update

McCalley, David V.

Abstract

This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantages of HILIC are discussed, and also the actual and perceived disadvantages of the technique, and how the latter can be overcome. Some new applications of HILIC in the characterization of pharmaceuticals , where it can be applied even to the separation of intact proteins, and to applications in metabolomics, will be discussed.

• Journal Article Type: Article
• Acceptance Date: Feb 6, 2019
• Publication Date: Mar 1, 2019
• Deposit Date: Feb 21, 2019
• Publicly Available Date: Feb 22, 2019
• Journal: LC.GC Europe
• Print ISSN: 1471-6577
• Peer Reviewed: Peer Reviewed
• Volume: 32
• Issue: 3
• Pages: 114-125
• Keywords: high performance liquid chromatography, drugs, biopharmaceuticals, hydrophilic interactions
• Public URL: https://uwe-repository.worktribe.com/output/851199
• Publisher URL: http://www.chromatographyonline.com/hydrophilic-interaction-liquid-chromatography-update
• Additional Information: Additional Information : This is the author's accepted manuscript. The final published version is available here: http://www.chromatographyonline.com/hydrophilic-interaction-liquid-chromatography-update
• Contract Date: Feb 21, 2019