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Hydrophilic interaction liquid chromatography: An update

McCalley, David V.

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This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantages of HILIC are discussed, and also the actual and perceived disadvantages of the technique, and how the latter can be overcome. Some new applications of HILIC in the characterization of pharmaceuticals , where it can be applied even to the separation of intact proteins, and to applications in metabolomics, will be discussed.


McCalley, D. V. (2019). Hydrophilic interaction liquid chromatography: An update. Lc Gc Europe, 32(3), 114-125

Journal Article Type Article
Acceptance Date Feb 6, 2019
Publication Date Mar 1, 2019
Deposit Date Feb 21, 2019
Publicly Available Date Feb 22, 2019
Journal LC.GC Europe
Print ISSN 1471-6577
Peer Reviewed Peer Reviewed
Volume 32
Issue 3
Pages 114-125
Keywords high performance liquid chromatography, drugs, biopharmaceuticals, hydrophilic interactions
Public URL
Publisher URL
Additional Information Additional Information : This is the author's accepted manuscript. The final published version is available here:


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