Skip to main content

Research Repository

See what's under the surface

Advanced Search

A study of the analysis of acidic solutes by hydrophilic interaction chromatography

McCalley, David V.

Authors



Abstract

© 2017 Elsevier B.V. The analysis of acidic solutes was compared on a cross-linked bonded amino phase and a neutral hybrid inorganic-organic amide phase, previously shown to give reasonable retention of acidic solutes. The amino column gave strong selective retention of acids, which was governed by ionic interactions that mostly increased as the solute became more negatively charged at higher pH. While the relative selectivity of the amide column towards acids, bases and neutrals was completely different to that of the amino column, the selectivity of both columns towards acidic solutes alone was surprisingly similar. It is possible that solute charge also controls retention on the amide column, through increased solute hydrophilicity and increased hydrogen bonding between the ionised form of the acid and neutral polar column groups. On both these silica-based columns there appeared to be a competitive effect between repulsion of acidic solutes from silanols, which become increasingly ionised as the pH is raised. This effect was absent when using a polymer-based amino phase which has no silanols.

Journal Article Type Article
Publication Date Jan 26, 2018
Journal Journal of Chromatography A
Print ISSN 0021-9673
Electronic ISSN 1873-3778
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1534
Pages 64-74
APA6 Citation McCalley, D. V. (2018). A study of the analysis of acidic solutes by hydrophilic interaction chromatography. Journal of Chromatography A, 1534, 64-74. https://doi.org/10.1016/j.chroma.2017.12.045
DOI https://doi.org/10.1016/j.chroma.2017.12.045
Keywords hydrophilic interaction chromatography, retention, selectivity, acids
Publisher URL http://dx.doi.org/10.1016/j.chroma.2017.12.045

Files








You might also like



Downloadable Citations

;