But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing

Mccalley, David; Stoll, Dwight R

Abstract

Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process, "perfect peaks" are not observed often outside of textbooks. Several physico-chemical phenomena can lead to asymmetric peak shapes, including analyte adsorption to different types of sites within the stationary phase, and overload tailing, which may involve a variety of factors. Understanding these phenomena can help identify whether the cause of asymmetry is most likely to have a physical or chemical origin, which in turn dictates which troubleshooting steps to start with when dealing with poor peak shapes.

• Journal Article Type: Article
• Acceptance Date: Oct 15, 2021
• Online Publication Date: Nov 1, 2021
• Publication Date: Nov 1, 2021
• Deposit Date: Nov 13, 2021
• Publicly Available Date: Dec 2, 2021
• Journal: LCGC NORTH AMERICA
• Print ISSN: 1527-5949
• Electronic ISSN: 1939-1889
• Peer Reviewed: Peer Reviewed
• Volume: 39
• Issue: 11
• Pages: 526–533, 539
• Public URL: https://uwe-repository.worktribe.com/output/8073297
• Publisher URL: https://www.chromatographyonline.com/view/but-my-peaks-are-not-gaussian-part-iii-physicochemical-causes-of-peak-tailing