Professor David McCalley David.Mccalley@uwe.ac.uk
Professor in Bio-Analytical Science
But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing
Mccalley, David; Stoll, Dwight R
Authors
Dwight R Stoll
Abstract
Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process, "perfect peaks" are not observed often outside of textbooks. Several physico-chemical phenomena can lead to asymmetric peak shapes, including analyte adsorption to different types of sites within the stationary phase, and overload tailing, which may involve a variety of factors. Understanding these phenomena can help identify whether the cause of asymmetry is most likely to have a physical or chemical origin, which in turn dictates which troubleshooting steps to start with when dealing with poor peak shapes.
Citation
Mccalley, D., & Stoll, D. R. (2021). But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing. LCGC North America, 39(11), 526–533, 539
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 15, 2021 |
Online Publication Date | Nov 1, 2021 |
Publication Date | Nov 1, 2021 |
Deposit Date | Nov 13, 2021 |
Publicly Available Date | Dec 2, 2021 |
Journal | LCGC NORTH AMERICA |
Print ISSN | 1527-5949 |
Peer Reviewed | Peer Reviewed |
Volume | 39 |
Issue | 11 |
Pages | 526–533, 539 |
Public URL | https://uwe-repository.worktribe.com/output/8073297 |
Publisher URL | https://www.chromatographyonline.com/view/but-my-peaks-are-not-gaussian-part-iii-physicochemical-causes-of-peak-tailing |
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But my peaks are not Gaussian! Part III – Physico-chemical causes of peak tailing
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Copyright Statement
This is the author's accepted manuscript. The final published version is available here: https://www.chromatographyonline.com/view/but-my-peaks-are-not-gaussian-part-iii-physicochemical-causes-of-peak-tailing
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