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DNA barcoding the native flowering plants and conifers of wales (2012)
Journal Article
Rich, T. C., Steinke, D., de Vere, N., Ford, C. R., Trinder, S. A., Long, C., …Wilkinson, M. J. (2012). DNA barcoding the native flowering plants and conifers of wales. PLoS ONE, 7(6), e37945. https://doi.org/10.1371/journal.pone.0037945

We present the first national DNA barcode resource that covers the native flowering plants and conifers for the nation of Wales (1143 species). Using the plant DNA barcode markers rbcL and matK, we have assembled 97.7% coverage for rbcL, 90.2% for ma... Read More about DNA barcoding the native flowering plants and conifers of wales.

Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate (2012)
Journal Article
Allainguillaume, J., Ellison, A., Allainguillaume, J., Girdwood, S., Pachebat, J., Peat, K. M., …Consuegra, S. (2012). Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate. Proceedings of the Royal Society B: Biological Sciences, 279(1749), 5004-5013. https://doi.org/10.1098/rspb.2012.1929

Major histocompatibility complex (MHC) genes encode proteins that present pathogen-derived antigens to T-cells, initiating the adaptive immune response in vertebrates. Although populations with low MHC diversity tend to be more susceptible to pathoge... Read More about Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate.

Exploiting the brachypodium tool box in cereal and grass research (2011)
Journal Article
Mur, L. A., Allainguillaume, J., Catalán, P., Hasterok, R., Jenkins, G., Lesniewska, K., …Vogel, J. (2011). Exploiting the brachypodium tool box in cereal and grass research. New Phytologist, 191(2), 334-347. https://doi.org/10.1111/j.1469-8137.2011.03748.x

It is now a decade since Brachypodium distachyon (Brachypodium) was suggested as a model species for temperate grasses and cereals. Since then transformation protocols, large expressed sequence tag (EST) databases, tools for forward and reverse genet... Read More about Exploiting the brachypodium tool box in cereal and grass research.

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010 (2011)
Journal Article
Smulders, M. J., Reusch, T. B., Pinheiro, J. B., Nguyen, T. T., Messmer, A. M., Goodisman, M. A., …Zucchi, M. I. (2011). Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010. Molecular Ecology Resources, 11(1), 219-222. https://doi.org/10.1111/j.1755-0998.2010.02944.x

This article documents the addition of 229 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Acacia auriculiformis-×-Acacia mangium hybrid, Alabama argillacea, Anoplopoma fimbria, A... Read More about Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010.

Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops (2009)
Journal Article
Elliott, L. M., Mason, D. C., Allainguillaume, J., & Wilkinson, M. J. (2009). Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops. Journal of Applied Remote Sensing, 3(1), 033562. https://doi.org/10.1117/1.3269615

High resolution descriptions of plant distribution have utility for many ecological applications but are especially useful for predictive modeling of gene flow from transgenic crops. Difficulty lies in the extrapolation errors that occur when limited... Read More about Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops.

Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment (2009)
Journal Article
Allainguillaume, J., Harwood, T., Ford, C. S., Cuccato, G., Norris, C., Allender, C. J., …Wilkinson, M. J. (2009). Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment. New Phytologist, 183(4), 1201-1211. https://doi.org/10.1111/j.1469-8137.2009.02877.x

Biocontainment methods for genetically modified crops closest to commercial reality (chloroplast transformation, male sterility) would be compromised (in absolute terms) by seed-mediated gene flow leading to chloroplast capture. Even in these circums... Read More about Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment.

Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment (2009)
Journal Article
Haider, N., Allainguillaume, J., & Wilkinson, M. J. (2009). Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment. Current Genetics, 55(2), 139-150. https://doi.org/10.1007/s00294-009-0230-5

Environmental concerns over the cultivation of Genetically Modified (GM) crops largely centre on the ecological consequences following gene flow to wild relatives. One attractive solution is to deploy biocontainment measures that prevent hybridizatio... Read More about Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment.

Transmission of cocoa swollen shoot virus by seeds (2008)
Journal Article
Quainoo, A. K., Wetten, A. C., & Allainguillaume, J. (2008). Transmission of cocoa swollen shoot virus by seeds. Journal of Virological Methods, 150(1-2), 45-49. https://doi.org/10.1016/j.jviromet.2008.03.009

A study was undertaken to determine whether cocoa swollen shoot virus is transmitted by seeds, to improve the robustness of quarantine procedures for international exchange and long term conservation of cocoa germplasm. PCR/capillary electrophoresis,... Read More about Transmission of cocoa swollen shoot virus by seeds.

The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees (2008)
Journal Article
Quainoo, A. K., Wetten, A. C., & Allainguillaume, J. (2008). The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, 149(1), 91-96. https://doi.org/10.1016/j.jviromet.2008.01.007

Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSS... Read More about The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees.

Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives (2007)
Journal Article
Allender, C. J., Allainguillaume, J., Lynn, J., & King, G. J. (2007). Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives. TAG Theoretical and Applied Genetics, 114(4), 609-618. https://doi.org/10.1007/s00122-006-0461-5

Diversity in the chloroplast genome of 171 accessions representing the Brassica 'C' (n = 9) genome, including domesticated and wild B. oleracea and nine inter-fertile related wild species, was investigated using six chloroplast SSR (microsatellite) m... Read More about Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives.

Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea (2006)
Journal Article
Wilkinson, M. J., Allender, C. J., Ford, C. S., Allainguillaume, J., Grilli-Chantler, P., & Cuccato, G. (2006). Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea. Proceedings of the Royal Society B: Biological Sciences, 273(1605), 3111-3115. https://doi.org/10.1098/rspb.2006.3686

Research on the environmental risks of gene flow from genetically modified (GM) crops to wild relatives has traditionally emphasized recipients yielding most hybrids. For GM rapeseed (Brassica napus), interest has centred on the 'frequently hybridizi... Read More about Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea.

Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats (2006)
Journal Article
Allainguillaume, J., Alexander, M., Bullock, J. M., Saunders, M., Allender, C. J., King, G., …Wilkinson, M. J. (2006). Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats. Molecular Ecology, 15(4), 1175-1184. https://doi.org/10.1111/j.1365-294X.2006.02856.x

Fitness of hybrids between genetically modified (GM) crops and wild relatives influences the likelihood of ecological harm. We measured fitness components in spontaneous (non-GM) rapeseed x Brassica rapa hybrids in natural populations. The F1 hybrids... Read More about Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats.

Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA (2005)
Journal Article
Renau-Morata, B., Nebauer, S. G., Sales, E., Allainguillaume, J., Caligari, P., & Segura, J. (2005). Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA. American Journal of Botany, 92(5), 875-884. https://doi.org/10.3732/ajb.92.5.875

Cedrus atlantica (Pinaceae) is a large and exceptionally long-lived conifer native to the Rif and Atlas Mountains of North Africa. To assess levels and patterns of genetic diversity of this species, samples were obtained throughout the natural range... Read More about Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA.

The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model (2004)
Journal Article
Elliott, L. J., Mason, D. C., Wilkinson, M. J., Allainguillaume, J., Norris, C., Alexander, M., & Welters, R. (2004). The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model. Journal of Applied Ecology, 41(6), 1174-1184. https://doi.org/10.1111/j.0021-8901.2004.00963.x

1. There is concern over the possibility of unwanted environmental change following transgene movement from genetically modified (GM) rapeseed Brassica napus to its wild and weedy relatives. 2. The aim of this research was to develop a remote sensing... Read More about The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model.

Hybridization Between Brassica napus and B. rapa on a National Scale in the United Kingdom (2003)
Journal Article
Shaw, M. W., Wilkinson, M. J., Elliott, L. J., Allainguillaume, J., Norris, C., Welters, R., …Mason, D. C. (2003). Hybridization Between Brassica napus and B. rapa on a National Scale in the United Kingdom. Science, 302(5644), 457-459. https://doi.org/10.1126/science.1088200

Measures blocking hybridization would prevent or reduce biotic or environmental change caused by gene flow from genetically modified (GM) crops to wild relatives. The efficacy of any such measure depends on hybrid numbers within the legislative regio... Read More about Hybridization Between Brassica napus and B. rapa on a National Scale in the United Kingdom.

A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors (2000)
Journal Article
Wilkinson, M. J., Davenport, I. J., Charters, Y. M., Jones, A. E., Allainguillaume, J., Butler, H. T., …Raybould, A. F. (2000). A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors. Molecular Ecology, 9(7), 983-991. https://doi.org/10.1046/j.1365-294X.2000.00986.x

One of the major environmental concerns over genetically modified (GM) crops relates to transgene movement into wild relatives. The pattern of hybridization ultimately affects the scale and rapidity of ecological change and the feasibility of contain... Read More about A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors.

Quantifying gene movement from oilseed rape to its wild relatives using remote sensing (2000)
Journal Article
Davenport, I. J., Wilkinson, M. J., Mason, D. C., Charters, Y. M., Jones, A. E., Allainguillaume, J., …Raybould, A. F. (2000). Quantifying gene movement from oilseed rape to its wild relatives using remote sensing. International Journal of Remote Sensing, 21(18), 3567-3573. https://doi.org/10.1080/014311600750037598

The potential environmental risks due to gene flow from genetically modified (GM) crops into wild plant populations are currently of great concern. Of particular interest is gene flow from GM oilseed rape (Brassica napus) into its wild parental speci... Read More about Quantifying gene movement from oilseed rape to its wild relatives using remote sensing.

Genetic diversity and pedigree for Sophora toromiro (Leguminosae): A tree extinct in the wild (1999)
Journal Article
Maunder, M., Culham, A., Bordeu, A., Allainguillaume, J., & Wilkinson, M. J. (1999). Genetic diversity and pedigree for Sophora toromiro (Leguminosae): A tree extinct in the wild. Molecular Ecology, 8(5), 725-738

Sophora toromiro was endemic to the Pacific island of Papa Nui (Easter Island) and is extinct in the wild. The species has survived in private and botanic gardens. The species is the subject of an international study to support its conservation ex si... Read More about Genetic diversity and pedigree for Sophora toromiro (Leguminosae): A tree extinct in the wild.

Evidence that genes from the male parent may influence the morphology of potato dihaploids (1997)
Journal Article
Barr, S. N., Allainguillaume, J., Wilkinson, M. J., Clulow, S. A., & Barr, S. N. R. (1997). Evidence that genes from the male parent may influence the morphology of potato dihaploids. TAG Theoretical and Applied Genetics, 94(2), 241-248. https://doi.org/10.1007/s001220050406

A number of recent studies have provided evidence that potato dihaploids (S. tuberosum) contain and express DNA front the male (dihaploid inducer) parent, S. phureja. The importance of this for breeding programmes that use dihaploid potatoes is to so... Read More about Evidence that genes from the male parent may influence the morphology of potato dihaploids.

Evidence for somatic translocation during potato dihaploid induction (1995)
Journal Article
Wilkinson, M. J., Bennett, S. T., Clulow, S. A., Allainguillaume, J., Harding, K., & Bennett, M. D. (1995). Evidence for somatic translocation during potato dihaploid induction. Heredity, 74(2), 146-151. https://doi.org/10.1038/hdy.1995.21

Potato dihaploid PDH55 (Solatium tuberosum) is exclusively euploid (2n = 24) but apparently contains and expresses DNA from dihaploid inducer IVP48 (S. phureja). Genomic in situ hybridization (GISH) suggested IVP48 DNA incorporated stably into PDH55... Read More about Evidence for somatic translocation during potato dihaploid induction.