Managing sample introduction problems in hydrophilic interaction liquid chromatography

Taylor, Mark R.; Kawakami, Jane; McCalley, David V.

doi:10.1016/j.chroma.2023.464006

All Outputs (3)

Practical examination of flow rate effects and influence of the stationary phase water layer on peak shape and retention in hydrophilic interaction liquid chromatography (2023)
Journal Article

The effect of flow velocity on retention and peak shape of neutral, acidic and basic probe compounds was studied using seven different UHPLC hydrophilic interaction chromatography (HILIC) columns. Surprisingly on some columns, the retention factor k... Read More about Practical examination of flow rate effects and influence of the stationary phase water layer on peak shape and retention in hydrophilic interaction liquid chromatography.

Understanding and managing peak shape for basic solutes in reversed-phase high performance liquid chromatography (2023)
Journal Article

While other separation mechanisms can challenge the dominance of reversed phase (for example in the separation of native proteins), reversed phase liquid chromatography is the method of choice for the analysis of a wide variety of samples. However, b... Read More about Understanding and managing peak shape for basic solutes in reversed-phase high performance liquid chromatography.

Managing sample introduction problems in hydrophilic interaction liquid chromatography (2023)
Journal Article

Sample injection can cause serious problems in hydrophilic interaction liquid chromatography (HILIC) when the injection solvent has higher elution strength than the mobile phase (mp). It can lead to asymmetric peak shapes and poor efficiency. The pro... Read More about Managing sample introduction problems in hydrophilic interaction liquid chromatography.