Body image pressures for heterosexual women are well established. However, lesbian body image is less well understood, while bisexual women have largely been overlooked with the psychological literature. Further, women's investment in ‘traditional’ appearance practices associated with femininity are underexplored. The current study explored differences between 472 heterosexual, lesbian, and bisexual women on measures of body satisfaction, body hair practices, and cosmetics use. While there were no significant differences between body satisfaction scores, lesbian and bisexual women had more positive attitudes to body hair, and were less likely to remove hair from particular parts of their bodies, than heterosexual women. Cosmetics use was highest among heterosexual women, significantly lower among bisexual women, and lowest among lesbians. We argue that these results highlight the importance of exploring the distinctiveness of bisexual, lesbian and heterosexual women's appearance concerns and appearance practices.