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MEF2A regulates mGluR-dependent AMPA receptor trafficking independently of Arc/Arg3.1

Carmichael, Ruth E; Wilkinson, Kevin A; Craig, Tim J.; Ashby, Michael C.; Henley, Jeremy M.

Authors

Ruth E Carmichael

Kevin A Wilkinson

Tim Craig Tim.Craig@uwe.ac.uk
AHoD in Biological Sciences

Michael C. Ashby

Jeremy M. Henley



Abstract

© 2018 The Author(s). Differential trafficking of AMPA receptors (AMPARs) to and from the postsynaptic membrane is a key determinant of the strength of excitatory neurotransmission, and is thought to underlie learning and memory. The transcription factor MEF2 is a negative regulator of memory in vivo, in part by regulating trafficking of the AMPAR subunit GluA2, but the molecular mechanisms behind this have not been established. Here we show, via knockdown of endogenous MEF2A in primary neuronal culture, that MEF2A is specifically required for Group I metabotropic glutamate receptor (mGluR)-mediated GluA2 internalisation, but does not regulate AMPAR expression or trafficking under basal conditions. Furthermore, this process occurs independently of changes in expression of Arc/Arg3.1, a previously characterised MEF2 transcriptional target and mediator of mGluR-dependent long-term depression. These data demonstrate a novel MEF2A-dependent mechanism for the regulation of activity-dependent AMPAR trafficking.

Citation

Carmichael, R. E., Wilkinson, K. A., Craig, T. J., Ashby, M. C., & Henley, J. M. (2018). MEF2A regulates mGluR-dependent AMPA receptor trafficking independently of Arc/Arg3.1. Scientific Reports, 8(1), 5263. https://doi.org/10.1038/s41598-018-23440-0

Journal Article Type Article
Acceptance Date Mar 13, 2018
Publication Date Dec 1, 2018
Journal Scientific Reports
Electronic ISSN 2045-2322
Publisher Nature Research (part of Springer Nature)
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Pages 5263
DOI https://doi.org/10.1038/s41598-018-23440-0
Keywords AMPA receptor
MEF2A
Synaptic transmission
Public URL https://uwe-repository.worktribe.com/output/870437
Publisher URL https://www.nature.com/articles/s41598-018-23440-0

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