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Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions

Heaton, James C.; Russell, Joseph J.; Underwood, Tim; Boughtflower, Robert; McCalley, David V.

Authors

James C. Heaton

Joseph J. Russell

Tim Underwood

Robert Boughtflower



Abstract

The retention and peak shape of neutral, basic and acidic solutes was studied on hydrophilic interaction chromatography (HILIC) stationary phases that showed both strong and weak ionic retention characteristics, using aqueous-acetonitrile mobile phases containing either formic acid (FA), ammonium formate (AF) or phosphoric acid (PA). The effect of organic solvent concentration on the results was also studied. Peak shape was good for neutrals under most mobile phase conditions. However, peak shapes for ionised solutes, particularly for basic compounds, were considerably worse in FA than AF. Even neutral compounds showed deterioration in performance with FA when the mobile phase water concentration was reduced. The poor performance in FA cannot be entirely attributed to the negative impact of ionic retention on ionised silanols on the underlying silica base materials, as results using PA at lower pH (where their ionisation is suppressed) were inferior to those in AF. Besides the moderating influence of the salt cation on ionic retention, it is likely that salt buffers improve peak shape due to the increased ionic strength of the mobile phase and its impact on the formation of the water layer on the column surface. © 2014 Elsevier B.V.

Journal Article Type Article
Publication Date Jun 20, 2014
Journal Journal of Chromatography A
Print ISSN 0021-9673
Electronic ISSN 1873-3778
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1347
Pages 39-48
APA6 Citation Heaton, J. C., Russell, J. J., Underwood, T., Boughtflower, R., & McCalley, D. V. (2014). Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions. Journal of Chromatography A, 1347, 39-48. https://doi.org/10.1016/j.chroma.2014.04.026
DOI https://doi.org/10.1016/j.chroma.2014.04.026
Keywords HILIC, buffers, column efficiency, hydride phases
Publisher URL http://dx.doi.org/10.1016/j.chroma.2014.04.026
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